Anadolu Mandası Dışkılarından İzole Edilen Termofilik Campylobacter Türlerinin Moleküler Karakterizasyonu

Yıl 2020, Cilt: 5 Sayı: 1, 86 - 92, 31.03.2020

Timur Gülhan , Merve Gizem Sezener , Serhan Akgöz , Volkan Ergüden , Arzu Findik , Alper Çiftci

https://doi.org/10.35229/jaes.688263

Öz

Mandaların diğer hayvanlarda olduğu gibi bazı hastalıkların duyarlı hayvan popülasyonlarına ve insanlara bulaştırılmasında rol oynadıkları ortaya konulmuştur. Bu çalışmada Amasya ili ve ilçelerinde yetiştiriciliği yapılmakta olan Anadolu Mandalarından toplanan 140 dışkı örneği termofilik campylobacter türleri açısından incelendi. Dışkı örneklerinden termofilik campylobacter türlerinin izolasyonu amacıyla standart selektif zenginleştirme tekniği kullanıldı. Campylobacter şüpheli izolatların cins ve tür düzeyinde identifikasyonları PCR ile yapıldı. 140 dışkı örneğinden 13 (%9.3) termofilik campylobacter cins düzeyinde identifiye edildi. İdentifiye edilen 13 izolatın 2 (%15.4)’si C. jejuni, 1 (%7.7)’i C. coli olarak isimlendirilirken, geriye kalan 10 izolat Campylobacter spp. olarak tanımlandı. C. jejuni izolatlarında ctx (cytolethal distenting toxin) genlerinin (cdtA, cdtB ve cdtC) tespiti mPCR ile gerçekleştirildi. İzolatların hiç birinde cdt geni saptanamadı. Bu araştırma ile bölgemizde ilk kez Anadolu Mandalarından sağlanan dışkı örnekleri termofilik campylobacter türleri yönünden incelendi. Araştırmadan elde edilen verilerin, yöremizde yapılacak benzer çalışmalara kaynak teşkil edebileceği kanısına varıldı.

Anahtar Kelimeler

Anadolu Mandası, Dışkı, PCR

Destekleyen Kurum

Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

Proje Numarası

PYO.VET. 1904.18.019

Teşekkür

Bu çalışmaya PYO.VET. 1904.18.019 nolu proje kapsamında maddi destek sağlayan Ondokuz Mayıs Üniversitesi Bilimsel Araştırma Projeleri Başkanlığınca teşekkür ederiz.

Molecular Characterization of Thermophilic Campylobacter Species Isolated From Anatolian Buffalo Feces

Öz

Buffaloes as in other animals have been demonstrated to play a role in certain diseases transmitted to susceptible animals and human populations. In this study, 140 fecal samples collected from Anatolian Buffaloes in breeding Amasya and around were examined for thermophilic campylobacter species. Standard selective enrichment methods were used for isolation of thermofhilic campylobacter species from fecal samples. Identification of campylobacter suspected isolates at the genus and species levels was performed by PCR. Of the 140 stool samples, 13 (9.3%) thermophilic campylobacter were identified at the genus level. While 2 (15.4%) of the 13 isolated isolates were named C. jejuni and 1 (7.7%) as C. coli, the remaining 10 isolates were defined as Campylobacter spp. Detection of ctx (cytolethal distenting toxin) genes (cdtA, cdtB and cdtC) in C. jejuni isolate was performed by mPCR. The ctx genes were not detected in any of the isolates. Fecal samples obtained from Anatolian Buffaloes were examined first time in our region respect to thermophilic campylobacter species. We concluded that the data obtained of research can constitute a resource to similar studies in our region.

Anahtar Kelimeler

Anatolian Buffaloes, Feces, PCR

Proje Numarası

PYO.VET. 1904.18.019

Kaynakça

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