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Spodoptera frugiperda Hücre Kültüründe Geliştirilen Autographa californica nükleer polihedrosis virüsünün (AcNPV) Replikasyonu Üzerine Türkiye Propolisinin Antiviral Etkisi

Year 2022, , 69 - 81, 30.06.2022
https://doi.org/10.35206/jan.1053371

Abstract

Bu çalışmada, Spodoptera frugiperda kültüründe geliştirilen Autographa californica nükleer polihedrozis virüsünün (AcNPV) replikasyonu üzerine Türkiye'den elde edilen propolis ekstraktlarının etkisi, sitopatik etkiler, hidroksil radikal aracılı DNA hasarı ve progeni virüs konsantrasyonundaki değişiklikler incelenerek araştırılmıştır. Propolis özü kullanarak sitopatik değişiklikler ve DNA hasarı üzerinde engelleyici etki bulduk. Propolis ekstraktlarının sitopatik değişiklikler ve hidroksil radikal aracılı DNA hasarı üzerinde kontrole benzer olduğu bulundu. Sf9 hücrelerinde emilim bölgesi üretimi açısından, propolis konsantrasyonu 3.125 ve 1.5625 µg/mL olduğunda kontamine kontrol hücrelerinde kontamine hücrelerin ortalama yüzdesi arasında önemli bir fark bulunmadı (sırasıyla 4.018 (plak oluşturan birim) (PFU) pfu/mL ve 5.145 pfu/mL) (p<0.05) ve kontamine kontrol hücrelerindeki ortalama emilim bölgesi sayısı ile propolis konsantrasyonundaki kontamine hücreler arasında anlamlı bir fark bulunmadı. 100, 50 μg/mL propolis ekstraktlarının konsantrasyonu 1 h pi dahil edildiğinde propolis ekstraktları ile virüs titresinde sırasıyla %98.70 ve %98.67 azalma bulundu. (p<0.05). Bununla birlikte, farklı propolis özütü konsantrasyonları, virüslerin sayısını önemli ölçüde azaltmıştır.

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References

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  • Asmafiliz, N., Kılıç, Z., Öztürk, A., Süzen, Y., Hökelek, T., & Açık, L. (2015). Phosphorus-Nitrogen Compounds: Part 25. Syntheses, Spectroscopic, Structural and Electrochemical Investigations, Antimicrobial Activities, and DNA Interactions of Ferrocenyl di amino cyclotri phosphazenes. Phosphorus Sulfur Silicon Relat Elemtary, 188, 1723-1742.
  • Banskota A.H., Nagaoka, T., Sumioka, L.Y., Tezuka, Y., Awale, S., Midorikawa, K., Matsushige, K., & Kadota, S. (2002). Antiproliferative activity of the Netherlands propolis and its active principles in cancer cell lines. Journal of Ethnopharmacology, 80(1), 67-73.
  • Blissard, G., Black, B., Crook, N., Keddie, B.A., Possee, R., & Rohrmann, G. (2000). Virus Taxonomy, Classification and Nomenclature of Viruses. Seventh report of the International Committee for the Taxonomy of Viruses. in M.H.V van Regenmortel et al. (eds.), Academic Press, San Diego, Califrnia, pp, 195–202.
  • Bischoff, D. S., & Slavicek, J. M. (1996). Characterization of the Lymantria dispar nucleopolyhedrovirus 25K FP gene. Journal of General Virology, 77, 1913–1923.
  • Boeru. V., & Derevici, A. (1978). Some chemical and physical data on Romania propolis. Apimondia "propolis" Bucharest. Evidence-Based Complementary and Alternative Medicine, 1978, 19-26.
  • Castrillo, J. L., Vanden, B. D., & Carrasco, L. (1986). 3-Methyl quercetin is a potent and selective inhibitor of poliovirus RNA synthesis. Virology, 152, 219-227.
  • Custódio, L., Escapa, A. L., Fajardo, A., Aligue, R., Neng, N., & Florecirc, J. M. (2011). In vitro cytotoxic effects and apoptosis induction by a methanol leaf extract of carob tree (Ceratonia siliqua L.). Journal of Medicinal Plants Research, 5, 1987-1996.
  • Castaldo S., & Capasso F. (2002). Propolis, an old remedy used in modern medicine. Fitoterapia, 73(1), S1-S6.
  • Duman, M., & Ozpolat, E. (2015). Effects of water extract of propolis on fresh shibuta (Barbus grypus) fillets during chilled storage. Food Chemistry, 189, 80-85.
  • Engelhard, E. K., Kam-Morgan, L. N. W., Washburn, J. O., & Volkman, L. E. (1994). The insect tracheal system: A conduit for the sytemic spread of Autographa californica M nuclear polyhedrosis virus. Proceedings of the National Academy of Sciences, 91, 3224-3227.
  • Erturk, O., Demirbag, Z., & Beldus, A. O. (2000). Antiviral activity of some plant extracts on the replication of Autographa californica nuclear polyhedrosis virus. Turkish Journal of Biology, 24, 833-844.
  • Ertürk, Ö., Çil, E., Yoloğlu, N., & Yavuz, C. (2016). An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. Mellifera, 16, 4-18. Gad, S. C. (1999). Multiple Chemical Sensitivity: A Moderator's Viewpoint. International Journal of Toxicology, 18, 379-381.
  • Granados, R. R., & Lawler, K. A. (1981). In vivo pathway of Autographa californica baculovirus invasion and infection. Virology, 108, 297–308.
  • Horton, H. M., & Burand, J. P. (1993). Saturable attachment sites for polyhedron-derived baculovirus on insect cells and evidence for entry via direct membrane fusion. Journal of Virology, 67, 1860-1868.
  • Kati, H., Ertürk, Ö., Demirbag, Z., & Belduz, A. O. (2001). Antiviral activity of Primula longipes extracts against baculovirus. Biologia Bratislava, 56, 633-636.
  • Kaul, T. N., Middleton, E., & Ogra, P. L. (1985). Antiviral effect of flavonoids on human viruses. Journal of Medicinal Virology, 15, 71-79.
  • Kumazawa S., Hamasaka T., & Nakayama, T. (2004). Antioxidant activity of propolis of various geographic origins. Food Chemistry, 84(3), 329-339.
  • Marcucci, M. C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26, 83-99.
  • Matsuda, S. (1994). Propolis health care food. Foods and Food Ingredients. Journal of Japan, 160, 64-73.
  • Mosmann, T. (1983) Rapid colorometric assay for cellular growth and survival: Aplication to proliferation and cytotoxicity assay. Journal of Immunology, 65,55-63. Motulsky, H. (2007). GraphPad Prism®Version 5.0 Statistics Guide. San Diego CA, GraphPad Software.
  • Oktar, N. (2009). K562 hücre dizisinde fosfin bileşiklerinin sitotoksik etkisinin MTT (3-(4,5 dimethylthiazole-2 yl)-2,5- diphenyltetrazolium bromide; thiazolyl blue) ile araştırılması. Çukurova Üniversitesi Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi, Adana. s. 47.
  • Petcharawan, O., Paitoon, N., Sripaiboon, P., & Saelee, S. (2012). Antiviral activity of crude hexane extracts from Allamanda cathartica on the replication of Autographa californica multiple nucleopolyhedrovirus. Current Applied Science and Technology, 12, 21-29.
  • Reed, L. J., & Muench, H. A. (1938). Simple method of estimating fifty per cent endpoints. American Journal of Epidemiology, 27, 493-497.
  • Saoo, K., Miki, H., & Ohmoi, M. (1996). Antiviral activity of Aloe extracts against Cytomegalovirus. Phytotheraphy Research, 10, 348-350.
  • Sökmen, A. (2001). Antiviral and cytotoxic activities of extracts from the cell culture and respective parts of some Turkish medicinal plants. Turkish Journal of Biology, 25, 343-350.
  • Vanhaelen, M., & Vanhaelen-Fastre, R. (1979). Propolis-origine, micrographie, composition chimique et activité thérapeutique. Journal de Pharmacie Belgique, 34, 253-259.
  • Wang, P., Hammer, D. A., & Granados, R. R. (1994). Interaction of Trichoplusia ni granulosis virus-encoded enhancin with the midgut epithelium and peritrophic membrane of four lepidopteran insects. Journal of General Virology, 75, 1961–1967.

The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture

Year 2022, , 69 - 81, 30.06.2022
https://doi.org/10.35206/jan.1053371

Abstract

In this study, the influence of propolis extracts gathered from Turkey on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) developed in Spodoptera frugiperda culture was researched through examining changes in cytopathic influences, hydroxyl radical mediated DNA damage and progeny virus concentration. By making use of propolis extract, we found inhibiting effect on cytopathic changes and DNA damage. It was found that propolis extracts on the cytopathic changes and hydroxyl radical-mediated DNA damage were similar to the control. The results showed that propolis has a protective effect on DNA exposed with H2O2. In terms of the production of occlusion bodies in Sf9 cells, no important difference was found between the average percentage of contaminated cells in contaminated control cells when the concentration of propolis is 3.125, and 1.5625 μg/mL (4.018 (Plaque forming units (PFU) pfu/mL and 5.145 pfu/mL respectively) (p<0.05) and no significant difference was found between the average number of occlusion bodies in the contaminated control cells and the contaminated cells in the concentration of propolis. Respectively 98.70% and 98.67% reduction were found in virus titter with propolis extracts when the concentration of propolis extracts 100, 50 μg/mL were included 1 h p.i. (p<0.05). However, different concentrations of propolis extract reduced the number of progeny viruses significantly.

References

  • Akbaş, H., Okumuş, A., Kılıç, Z., Hökelek, T., Süzen, Y., & Koç, Y. (2013). Phosphorusenitrogen compounds part 27. Syntheses, structural characterizations, antimicrobial and cytotoxic activities, and DNA interactions of new phosphazenes bearing secondary amino and pendant (4-fluorobenzyl) spiro groups. European Journal of Medicinal Chemistry, 70, 294-307.
  • Asmafiliz, N., Kılıç, Z., Öztürk, A., Süzen, Y., Hökelek, T., & Açık, L. (2015). Phosphorus-Nitrogen Compounds: Part 25. Syntheses, Spectroscopic, Structural and Electrochemical Investigations, Antimicrobial Activities, and DNA Interactions of Ferrocenyl di amino cyclotri phosphazenes. Phosphorus Sulfur Silicon Relat Elemtary, 188, 1723-1742.
  • Banskota A.H., Nagaoka, T., Sumioka, L.Y., Tezuka, Y., Awale, S., Midorikawa, K., Matsushige, K., & Kadota, S. (2002). Antiproliferative activity of the Netherlands propolis and its active principles in cancer cell lines. Journal of Ethnopharmacology, 80(1), 67-73.
  • Blissard, G., Black, B., Crook, N., Keddie, B.A., Possee, R., & Rohrmann, G. (2000). Virus Taxonomy, Classification and Nomenclature of Viruses. Seventh report of the International Committee for the Taxonomy of Viruses. in M.H.V van Regenmortel et al. (eds.), Academic Press, San Diego, Califrnia, pp, 195–202.
  • Bischoff, D. S., & Slavicek, J. M. (1996). Characterization of the Lymantria dispar nucleopolyhedrovirus 25K FP gene. Journal of General Virology, 77, 1913–1923.
  • Boeru. V., & Derevici, A. (1978). Some chemical and physical data on Romania propolis. Apimondia "propolis" Bucharest. Evidence-Based Complementary and Alternative Medicine, 1978, 19-26.
  • Castrillo, J. L., Vanden, B. D., & Carrasco, L. (1986). 3-Methyl quercetin is a potent and selective inhibitor of poliovirus RNA synthesis. Virology, 152, 219-227.
  • Custódio, L., Escapa, A. L., Fajardo, A., Aligue, R., Neng, N., & Florecirc, J. M. (2011). In vitro cytotoxic effects and apoptosis induction by a methanol leaf extract of carob tree (Ceratonia siliqua L.). Journal of Medicinal Plants Research, 5, 1987-1996.
  • Castaldo S., & Capasso F. (2002). Propolis, an old remedy used in modern medicine. Fitoterapia, 73(1), S1-S6.
  • Duman, M., & Ozpolat, E. (2015). Effects of water extract of propolis on fresh shibuta (Barbus grypus) fillets during chilled storage. Food Chemistry, 189, 80-85.
  • Engelhard, E. K., Kam-Morgan, L. N. W., Washburn, J. O., & Volkman, L. E. (1994). The insect tracheal system: A conduit for the sytemic spread of Autographa californica M nuclear polyhedrosis virus. Proceedings of the National Academy of Sciences, 91, 3224-3227.
  • Erturk, O., Demirbag, Z., & Beldus, A. O. (2000). Antiviral activity of some plant extracts on the replication of Autographa californica nuclear polyhedrosis virus. Turkish Journal of Biology, 24, 833-844.
  • Ertürk, Ö., Çil, E., Yoloğlu, N., & Yavuz, C. (2016). An In vitro Study on Antimicrobial and Antioxidant Activity of Propolis from Rize Province of Turkey. Mellifera, 16, 4-18. Gad, S. C. (1999). Multiple Chemical Sensitivity: A Moderator's Viewpoint. International Journal of Toxicology, 18, 379-381.
  • Granados, R. R., & Lawler, K. A. (1981). In vivo pathway of Autographa californica baculovirus invasion and infection. Virology, 108, 297–308.
  • Horton, H. M., & Burand, J. P. (1993). Saturable attachment sites for polyhedron-derived baculovirus on insect cells and evidence for entry via direct membrane fusion. Journal of Virology, 67, 1860-1868.
  • Kati, H., Ertürk, Ö., Demirbag, Z., & Belduz, A. O. (2001). Antiviral activity of Primula longipes extracts against baculovirus. Biologia Bratislava, 56, 633-636.
  • Kaul, T. N., Middleton, E., & Ogra, P. L. (1985). Antiviral effect of flavonoids on human viruses. Journal of Medicinal Virology, 15, 71-79.
  • Kumazawa S., Hamasaka T., & Nakayama, T. (2004). Antioxidant activity of propolis of various geographic origins. Food Chemistry, 84(3), 329-339.
  • Marcucci, M. C. (1995). Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26, 83-99.
  • Matsuda, S. (1994). Propolis health care food. Foods and Food Ingredients. Journal of Japan, 160, 64-73.
  • Mosmann, T. (1983) Rapid colorometric assay for cellular growth and survival: Aplication to proliferation and cytotoxicity assay. Journal of Immunology, 65,55-63. Motulsky, H. (2007). GraphPad Prism®Version 5.0 Statistics Guide. San Diego CA, GraphPad Software.
  • Oktar, N. (2009). K562 hücre dizisinde fosfin bileşiklerinin sitotoksik etkisinin MTT (3-(4,5 dimethylthiazole-2 yl)-2,5- diphenyltetrazolium bromide; thiazolyl blue) ile araştırılması. Çukurova Üniversitesi Sağlık Bilimleri Enstitüsü, Yüksek Lisans Tezi, Adana. s. 47.
  • Petcharawan, O., Paitoon, N., Sripaiboon, P., & Saelee, S. (2012). Antiviral activity of crude hexane extracts from Allamanda cathartica on the replication of Autographa californica multiple nucleopolyhedrovirus. Current Applied Science and Technology, 12, 21-29.
  • Reed, L. J., & Muench, H. A. (1938). Simple method of estimating fifty per cent endpoints. American Journal of Epidemiology, 27, 493-497.
  • Saoo, K., Miki, H., & Ohmoi, M. (1996). Antiviral activity of Aloe extracts against Cytomegalovirus. Phytotheraphy Research, 10, 348-350.
  • Sökmen, A. (2001). Antiviral and cytotoxic activities of extracts from the cell culture and respective parts of some Turkish medicinal plants. Turkish Journal of Biology, 25, 343-350.
  • Vanhaelen, M., & Vanhaelen-Fastre, R. (1979). Propolis-origine, micrographie, composition chimique et activité thérapeutique. Journal de Pharmacie Belgique, 34, 253-259.
  • Wang, P., Hammer, D. A., & Granados, R. R. (1994). Interaction of Trichoplusia ni granulosis virus-encoded enhancin with the midgut epithelium and peritrophic membrane of four lepidopteran insects. Journal of General Virology, 75, 1961–1967.
There are 28 citations in total.

Details

Primary Language English
Journal Section Research Articles
Authors

Ömer Ertürk 0000-0001-5837-6893

Ceren Başkan 0000-0001-7849-4459

Publication Date June 30, 2022
Published in Issue Year 2022

Cite

APA Ertürk, Ö., & Başkan, C. (2022). The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture. Journal of Apitherapy and Nature, 5(1), 69-81. https://doi.org/10.35206/jan.1053371
AMA Ertürk Ö, Başkan C. The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture. J.Apit.Nat. June 2022;5(1):69-81. doi:10.35206/jan.1053371
Chicago Ertürk, Ömer, and Ceren Başkan. “The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa Californica Nuclear Polyhedrosis Virus (AcNPV) Developed in Spodoptera Frugiperda Cell Culture”. Journal of Apitherapy and Nature 5, no. 1 (June 2022): 69-81. https://doi.org/10.35206/jan.1053371.
EndNote Ertürk Ö, Başkan C (June 1, 2022) The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture. Journal of Apitherapy and Nature 5 1 69–81.
IEEE Ö. Ertürk and C. Başkan, “The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture”, J.Apit.Nat., vol. 5, no. 1, pp. 69–81, 2022, doi: 10.35206/jan.1053371.
ISNAD Ertürk, Ömer - Başkan, Ceren. “The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa Californica Nuclear Polyhedrosis Virus (AcNPV) Developed in Spodoptera Frugiperda Cell Culture”. Journal of Apitherapy and Nature 5/1 (June 2022), 69-81. https://doi.org/10.35206/jan.1053371.
JAMA Ertürk Ö, Başkan C. The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture. J.Apit.Nat. 2022;5:69–81.
MLA Ertürk, Ömer and Ceren Başkan. “The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa Californica Nuclear Polyhedrosis Virus (AcNPV) Developed in Spodoptera Frugiperda Cell Culture”. Journal of Apitherapy and Nature, vol. 5, no. 1, 2022, pp. 69-81, doi:10.35206/jan.1053371.
Vancouver Ertürk Ö, Başkan C. The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture. J.Apit.Nat. 2022;5(1):69-81.
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