The Antiviral Effect of the Propolis from Turkey on the Replication of Autographa californica nuclear polyhedrosis virus (AcNPV) Developed in Spodoptera frugiperda Cell Culture
Abstract
In this study, the influence of propolis extracts gathered from Turkey on the replication of Autographa californica nuclear polyhedrosis virus (AcNPV) developed in Spodoptera frugiperda culture was researched through examining changes in cytopathic influences, hydroxyl radical mediated DNA damage and progeny virus concentration. By making use of propolis extract, we found inhibiting effect on cytopathic changes and DNA damage. It was found that propolis extracts on the cytopathic changes and hydroxyl radical-mediated DNA damage were similar to the control. The results showed that propolis has a protective effect on DNA exposed with H2O2. In terms of the production of occlusion bodies in Sf9 cells, no important difference was found between the average percentage of contaminated cells in contaminated control cells when the concentration of propolis is 3.125, and 1.5625 μg/mL (4.018 (Plaque forming units (PFU) pfu/mL and 5.145 pfu/mL respectively) (p<0.05) and no significant difference was found between the average number of occlusion bodies in the contaminated control cells and the contaminated cells in the concentration of propolis. Respectively 98.70% and 98.67% reduction were found in virus titter with propolis extracts when the concentration of propolis extracts 100, 50 μg/mL were included 1 h p.i. (p<0.05). However, different concentrations of propolis extract reduced the number of progeny viruses significantly.
Keywords
Baculovirus , Propolis , Antiviral activity , Virus replication
Kaynakça
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