Lamium galeobdolon (L.) L. ve Lamium purpureum L. Etanolik Ekstraktlarının Antimikrobiyal, Anti-quorum sensing ve Antibiyofilm Potansiyelleri

Year 2024, , 1 - 13, 30.06.2024

Ayşegül Akkoyunlu , Gorkem Dulger

https://doi.org/10.35206/jan.1457624

Abstract

Bakteriler, patojeniteyi kontrol etmek için quorum sensing (QS) adı verilen bir iletişim mekanizması yoluyla hareket eder. Biyofilm oluşumu QS mekanizması tarafından desteklenen bir süreçtir ve antibiyotik direncinde rol oynadığı bilinmektedir. Çalışmada Lamium galeobdolon ve Lamium purpureum bitkilerinden elde edilen etanol ekstraktlarının antimikrobiyal, anti-QS ve antibiyofilm kapasiteleri belirlendi. Bitki ekstraktlarının antimikrobiyal aktivitesi çeşitli hastane izolatlarına karşı kuyu difüzyon deneyi ile araştırılmıştır. Ekstraktlar, tüm bakteri ve mayalara karşı antimikrobiyal etki göstermiştir. L. purpureum'un 100 mg/mL dozunda Candida guilliermondii mayasına karşı 27.5 ± 0.71 mm'lik inhibitör etkisi dikkat çekicidir. Ekstraktların anti-QS potansiyeli, viyolasin pigment inhibisyonuna dayanan kuyu difüzyon deneyi ile değerlendirildi. L. purpureum ekstraktı, Chromobacterium violaceum ATCC 12472 biyosensör suşuna karşı daha yüksek seviyede anti-QS etkisi gösterdi. Biyofilm oluşturan Escherichia coli izolatına karşı antibiyofilm kapasitesi kristal viyole boyama yöntemiyle belirlendi. L. purpureum ekstraktı en yüksek dozda E. colibiyofilmine karşı %89.66 oranında inhibitör etki göstermiştir. Her iki bitkinin de anti-patojenik özelliklere sahip olduğu kanıtlanmıştır. Ancak L. purpureum bitkisinin, L. galeobdolon bitkisine göre anti-patojenik özelliği daha yüksek bir bitki olduğu söylenebilir.

Keywords

Antibiyofilm, Antimikrobiyal, Chromobacterium violaceum ATCC 12472, Lamium galeobdolon, Lamium purpureum

Antimicrobial, Anti-quorum sensing and Antibiofilm Potentials of Lamium galeobdolon (L.) L. and Lamium purpureum L. Ethanolic Extracts

Abstract

Bacteria act through a communication mechanism called quorum sensing (QS) to control pathogenicity. Biofilm formation is a process supported by the QS mechanism and is known to act a part in antibiotic resistance. In this study, antimicrobial, anti-QS and antibiofilm capacities of ethanol extracts obtained from Lamium galeobdolon (L.) L. and Lamium purpureum L. plants were determined. The antimicrobial activity of plant extracts was evaluated by well diffusion assay against various hospital isolates. The extracts have antimicrobial effects against all bacteria and yeasts. The 27.5 ± 0.71 mm inhibitory effect of L. purpureum against Candida guilliermondii yeast at a dose of 100 mg/mL is remarkable. The anti-QS potential of the extracts was evaluated by a well diffusion assay based upon violacein pigment inhibition. L. purpureum extract showed a higher level of anti-QS effect against Chromobacterium violaceum ATCC 12472 biosensor strain. Antibiofilm capacity against biofilm-forming Escherichia coli isolate was determined by the crystal violet staining method. L. purpureum extract showed an inhibitory effect of 89.66% against E.coli biofilm at the highest dose. Both plants have been shown to have anti-pathogenic properties. However, it can be said that the L.purpureum plant is a more highly anti-pathogenic plant compared to L. galeobdolon.

Keywords

Antibiofilm, Antimicrobial, Chromobacterium violaceum ATCC 12472, Lamium galeobdolon, Lamium purpureum

References

• Akkol, E. K., Yalçın, F. N., Kaya, D., Çalış, İ., Yesilada, E., & Ersöz, T. (2008). In vivo anti-inflammatory and antinociceptive actions of some Lamium species. Journal of Ethnopharmacology, 118(1), 166-172.
• Akkoyunlu, A., & Dulger, G. (2019). Chemical composition of Lamium purpureum L. and determination of anticancer activity of its essential oil on melanoma. Duzce University Journal of Science and Technology, 7(3), 1755-1763.
• Akkoyunlu, A., & Dulger, G. (2022). Chemical Composition and In Vitro Antimicrobial, Antioxidant, and Antiproliferative Studies of the Lamium galeobdolon L.(L.) Essential Oil. Russian Journal of Bioorganic Chemistry, 48(6), 1240-1246.
• Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants, 4(196), 2167-0412.
• Bali, E. B., Türkmen, K. E., Erdönmez, D., & Sağlam, N. (2019). Comparative study of inhibitory potential of dietary phytochemicals against quorum sensing activity of and biofilm formation by Chromobacterium violaceum 12472, and swimming and swarming behaviour of Pseudomonas aeruginosa PAO1. Food Technology and Biotechnology, 57(2), 212.
• Bubueanu, C., Iuksel, R., & Panteli, M. (2019). Haemostatic activity of butanolic extracts of Lamium album and Lamium purpureum aerial parts. Acta Pharmaceutica, 69(3), 443-449.
• D’Aquila, P., Sena, G., Crudo, M., Passarino, G., & Bellizzi, D. (2023). Effect of essential oils of Apiaceae, Lamiaceae, Lauraceae, Myrtaceae, and Rutaceae family plants on growth, biofilm formation, and quorum sensing in Chromobacterium violaceum, Pseudomonas aeruginosa, and Enterococcus faecalis. Microorganisms, 11(5), 1150.
• Dimitrova, P. D., Damyanova, T., & Paunova-Krasteva, T. (2023). Chromobacterium violaceum: A model for evaluating the anti-quorum sensing activities of plant substances. Scientia Pharmaceutica, 91(3), 33.
• Dulger, B., & Dulger, G. (2018). Anti-staphylococcal activity of Scutellaria albida subsp. albida against methicillin-resistant Staphylococcus aureus. Journal of Medicinal Plants Studies, 6(4), 27-30
• Dulger, G. (2022). Antimicrobial and Anticancer Activity of Corydalis solida. Value in Health Sciences, 12(3), 534-539.
• Elmanama, A. A., & Al-Reefi, M. R. (2017). Antimicrobial, anti-biofilm, anti-quorum sensing, antifungal and synergistic effects of some medicinal plants extracts. IUG Journal of Natural Studies, 25(2), 198-207.
• Haney, E. F., Trimble, M. J., & Hancock, R. E. (2021). Microtiter plate assays to assess antibiofilm activity against bacteria. Nature protocols, 16(5), 2615-2632.
• Lee, Y. C., Cho, S. G., Kim, S. W., & Kim, J. N. (2019). Anticariogenic potential of Korean native plant extracts against Streptococcus mutans. Planta medica, 85(16), 1242-1252.
• Lin, Y., Lou, K., Wu, G., Wu, X., Zhou, X., Feng, Y., ... & Yu, P. (2019). Bioactive metabolites in of Ginkgo biloba leaves: variations by seasonal, meteorological and soil. Brazilian Journal of Biology, 80, 790-797.
• Mirghani, R., Saba, T., Khaliq, H., Mitchell, J., Do, L., Chambi, L., ... & Rijal, G. (2022). Biofilms: Formation, drug resistance and alternatives to conventional approaches. AIMS microbiology, 8(3), 239.
• Mulya E., & Waturangi D. E. (2021). Screening and quantification of anti-quorum sensing and antibiofilm activity of Actinomycetes isolates against food spoilage biofilm-forming bacteria. BMC Microbiology, 21(1),1-8.
• Rios, J. L., & Recio, M. C. (2005). Medicinal plants and antimicrobial activity. Journal of ethnopharmacology, 100(1-2), 80-84.
• Salehi, B., Armstrong, L., Rescigno, A., Yeskaliyeva, B., Seitimova, G., Beyatli, A., ... & Sharifi-Rad, J. (2019). Lamium plants—A comprehensive review on health benefits and biological activities. Molecules, 24(10), 1913. Sharma, S., Mohler, J., Mahajan, S. D., Schwartz, S. A., Bruggemann, L., & Aalinkeel, R. (2023). Microbial biofilm: a review on formation, infection, antibiotic resistance, control measures, and innovative treatment. Microorganisms, 11(6), 1614.
• Tamfu, A. N., Ceylan, O., Fru, G. C., Ozturk, M., Duru, M. E., & Shaheen, F. (2020). Antibiofilm, antiquorum sensing and antioxidant activity of secondary metabolites from seeds of Annona senegalensis, Persoon. Microbial pathogenesis, 144, 104191.
• Terzić, J. T., Stanković, M. M., & Stefanović, O. D. (2023). Antibiofilm activity of Lamium album L. Extracts. Kragujevac Journal of Science, 45, 219-238.
• Vestby, L. K., Grønseth, T., Simm, R., & Nesse, L. L. (2020). Bacterial biofilm and its role in the pathogenesis of disease. Antibiotics, 9(2), 59.
• Yalçın, F. N., Kaya, D., Kılıç, E., Özalp, M., Ersöz, T., & Çalış, İ. (2007). Antimicrobial and Free Radical Scavenging Activities of Some Lamium Species from Turkey. Hacettepe University Journal of The Faculty of Pharmacy, (1), 11-22.