Antimicrobial, Anti-quorum sensing and Antibiofilm Potentials of Lamium galeobdolon (L.) L. and Lamium purpureum L. Ethanolic Extracts
Öz
Bacteria act through a communication mechanism called quorum sensing (QS) to control pathogenicity. Biofilm formation is a process supported by the QS mechanism and is known to act a part in antibiotic resistance. In this study, antimicrobial, anti-QS and antibiofilm capacities of ethanol extracts obtained from Lamium galeobdolon (L.) L. and Lamium purpureum L. plants were determined. The antimicrobial activity of plant extracts was evaluated by well diffusion assay against various hospital isolates. The extracts have antimicrobial effects against all bacteria and yeasts. The 27.5 ± 0.71 mm inhibitory effect of L. purpureum against Candida guilliermondii yeast at a dose of 100 mg/mL is remarkable. The anti-QS potential of the extracts was evaluated by a well diffusion assay based upon violacein pigment inhibition. L. purpureum extract showed a higher level of anti-QS effect against Chromobacterium violaceum ATCC 12472 biosensor strain. Antibiofilm capacity against biofilm-forming Escherichia coli isolate was determined by the crystal violet staining method. L. purpureum extract showed an inhibitory effect of 89.66% against E.coli biofilm at the highest dose. Both plants have been shown to have anti-pathogenic properties. However, it can be said that the L.purpureum plant is a more highly anti-pathogenic plant compared to L. galeobdolon.
Anahtar Kelimeler
Antibiofilm, Antimicrobial, Chromobacterium violaceum ATCC 12472, Lamium galeobdolon, Lamium purpureum
Kaynakça
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