Research Article

Comparison of Honey's Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye

Volume: 8 Number: 2 December 31, 2025
TR EN

Comparison of Honey's Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye

Abstract

The aim of this study is to determine and compare the physicochemical and biological characterization of some honey samples in the Eastern Black Sea Region of Türkiye. Physicochemical tests investigated the compliance of honey samples with the Turkish Food Codex. The phenolic components were analyzed by RP- HPLC-PDA. The total phenolic and flavonoid contents (TPC, TFC), antioxidant capacity (FRAP, DPPH), urease, glucose-oxidase, and antimicrobial activity (Disc diffusion, MIC) of the samples were determined, and FTIR and UV-Vis analyses were conducted. The spectra of the honey samples revealed the characteristic bands corresponding to the functional groups of minor components. As a result of the physicochemical analyses, it was determined that some of the samples examined did not comply with the standard values defined in the Turkish Food Codex in terms of the examined parameters. Spectral findings show that honey samples have a rich chemical composition. Anzer honey is preferable to other kinds with its rich phenolic and antioxidant content and relatively high antimicrobial activity.

Keywords

Flower honey , Black hive honey , Chestnut honey , Anzer honey

References

  1. Acar, V. (2021). Orta Asya Türklerinde bal ve balın tıbbi amaçlı kullanımı (Apiterapi). Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi, 11(3), 612-624.
  2. Acquarone, C., Buera, P., Elizalde, B. (2007). Pattern of pH and electrical conductivity upon honey dilution as a complementary tool for discriminating geographical origin of honeys. Food Chemistry, 101(2), 695-703.
  3. Akgün, N., Çelik, Ö. F., & Kelebekli, L. (2021). Physicochemical properties, total phenolic content, and antioxidant activity of chestnut, rhododendron, acacia and multifloral honey. Journal of Food Measurement and Characterization, 15(4), 3501-3508.
  4. Aksoy, Z. & Diğrak, M. (2006). Bingöl Yöresinde Toplanan Bal Ve Propolisin Antimikrobiyal Etkisi Üzerinde In Vitro Araştırmalar. Science and Engineering Journal of Fırat University, 18 (4), 471-478.
  5. Alan, Y., Atalan, E., Erbil, N., Bakir, O., Orman, Z., Kanik, P. (2014). Antimicrobial Activity of Honey and Propolis Collected In Muş and Bitlis Region, Muş Alparslan University Journal of Science, 2 (1), 221-229.
  6. Alothman, M., Bhat, R., Karim, A. A. (2009). Effect of radiation processing on phytochemicals and antioxidants in plant produce. Trends in Food Science & Technology, 20(4), 201–212.
  7. Ajlouni, S. & Sujirapinyokul, P. (2010). Hydroxymethylfurfuraldehyde and amylase contents in Australian honey. Food Chemistry, 119(3), 1000-1005. https://doi.org/10.1016/j.foodchem.2009.07.057
  8. Andrade, P., Ferreres F, Gil MI, Tomás-Barberán FA. (1997). Determination of phenolic compounds in honeys with different floral origin by capillary zone electrophoresis. Food Chemistry, 1, 79-81.
  9. Ateş, Y., & Yaşar, S. (2020). Chemical investigations on honey produced in Bingol and surroundings. Uludag Aricilik Dergisi, 20(1), 72-80.
  10. Bayram, N. (2021). Vitamin, mineral, polyphenol, amino acid profile of bee pollen from Rhododendron ponticum (source of “mad honey”): nutritional and 55 palynological approach. Journal of Food Measurement and Characterization, 15(3), 2659-2666.
APA
Çavuş, D., Ertürk, Ö., Çil, E., Can, Z., & Bağdatlı, E. (2025). Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye. Journal of Apitherapy and Nature, 8(2), 143-174. https://doi.org/10.35206/jan.1662563
AMA
1.Çavuş D, Ertürk Ö, Çil E, Can Z, Bağdatlı E. Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye. J.Apit.Nat. 2025;8(2):143-174. doi:10.35206/jan.1662563
Chicago
Çavuş, Demet, Ömer Ertürk, Elif Çil, Zehra Can, and Emine Bağdatlı. 2025. “Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye”. Journal of Apitherapy and Nature 8 (2): 143-74. https://doi.org/10.35206/jan.1662563.
EndNote
Çavuş D, Ertürk Ö, Çil E, Can Z, Bağdatlı E (December 1, 2025) Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye. Journal of Apitherapy and Nature 8 2 143–174.
IEEE
[1]D. Çavuş, Ö. Ertürk, E. Çil, Z. Can, and E. Bağdatlı, “Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye”, J.Apit.Nat., vol. 8, no. 2, pp. 143–174, Dec. 2025, doi: 10.35206/jan.1662563.
ISNAD
Çavuş, Demet - Ertürk, Ömer - Çil, Elif - Can, Zehra - Bağdatlı, Emine. “Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye”. Journal of Apitherapy and Nature 8/2 (December 1, 2025): 143-174. https://doi.org/10.35206/jan.1662563.
JAMA
1.Çavuş D, Ertürk Ö, Çil E, Can Z, Bağdatlı E. Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye. J.Apit.Nat. 2025;8:143–174.
MLA
Çavuş, Demet, et al. “Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye”. Journal of Apitherapy and Nature, vol. 8, no. 2, Dec. 2025, pp. 143-74, doi:10.35206/jan.1662563.
Vancouver
1.Demet Çavuş, Ömer Ertürk, Elif Çil, Zehra Can, Emine Bağdatlı. Comparison of Honey’s Physicochemical, Bioactive Components, and Biological Activities from the Eastern Black Sea Region of Türkiye. J.Apit.Nat. 2025 Dec. 1;8(2):143-74. doi:10.35206/jan.1662563