Dynamic thiol/disulphide homeostasis and ischemic modified albumin levels in idiopathic polyhydramnios

Abstract

Objective: The aim of study was to determine whether idiopathic polyhydramnios is in relation with dynamic thiol-disulfide homeostasis and ischemia modified albumin levels or not. Materials and methods: In this prospective case- control study, a total of 126 participants were included. The patient group consisted of 56 patients who were diagnosed idiopathic polyhydramnios, and the control group consisted of 56 healthy normal pregnant. Native thiol (-SH), total thiol (-SH. -SS), dynamic disulfide (-SS), values from maternal serum were measured and compared between groups. Results: 112 patients aged between 18-35 years, consisting of 56 idiopathic polyhydramnios and 56 control groups were included in the study. Maternal serum native and total thiol values were significantly higher in study group compared to control group (382.2 ±78.5 mmol/L vs. 331.8 ±43.9 mmol/L, p <0.001 and 435.2 ±76.2 mmol/L vs. 368.2 ±47.2 mmol/L, p<0.001). Disulphide / native thiol ratio and disulphide / total thiol ratio ratio was found to be statistically significantly higher (7.3 ±2.2 vs 5.5 ±0.9, p<0.001 and 6.3 ±1.7 vs 5.0 ±0.7, p<0.001), and native thiol/total thiol ratio ratio was significantly lower (87.4 ±3.4 vs 90.1 ±1.4, p<0.001) in control group. Mean cord blood ischemia modified albumin (IMA) was 0.69 ±0.02 Absorbance Unit, cord blood native thiol (SH) level 410.2 ±80.2, and cord blood total thiol level was 461.1 ±82.1 μmol/l in study group. All parameters except IMA and Native thiol / total thiol ratio were higher in cord blood samples of study group compared to control group. Conclusion: The thiol/disulfide balance shifted towards anti-oxidative status in pregnancies complicated with idiopathic polyhydramnios compared to control group.

Keywords

• IMA
• Thiol/disulphide homeostasis
• polyhydramnios

İdiyopatik polihidramniyoz olgularında dinamik tiyol/disülfit homeostazı ve iskemik modifiye albümin seviyeleri

Abstract

Amaç: Bu çalışmanın amacı, idiyopatik polihidramniosun dinamik tiyol-disülfit homeostazı ve iskemi modifiye albümin düzeyleri ile ilişkisinin olup olmadığını belirlemektir. Gereç ve Yöntemler: Bu prospektif vaka kontrol çalışmasına toplam 126 katılımcı dahil edildi. Hasta grubunu idiyopatik polihidramnios tanısı alan 56 hasta ve kontrol grubunu 56 sağlıklı normal gebe oluşturdu. Native tiyol (-SH), total tiyol (-SH.-SS), dinamik disülfid (-SS), maternal serum değerleri ölçüldü ve gruplar arasında karşılaştırıldı. Bulgular: Çalışmaya 56 idiyopatik polihidramnios ve 56 kontrol grubu olmak üzere 18-35 yaş arası 112 hasta dahil edildi. Maternal serum nativ ve total tiyol değerleri çalışma grubunda kontrol grubuna göre anlamlı derecede yüksekti (382,2 ±78,5 mmol/L - 331,8 ±43,9 mmol/L, p <0,001 ve 435,2 ±76,2 mmol/L - 368,2 ±47,2 mmol/ L, p<0.001). Disülfit/doğal tiyol oranı ve disülfür/toplam tiyol oranı istatistiksel olarak anlamlı derecede yüksek bulundu (7,3 ±2,2'ye karşı 5,5 ±0,9, p<0,001 ve 6,3 ±1,7'ye karşı 5,0 ±0,7, p<0,001) ve doğal tiyol/toplam tiol oranı oranı kontrol grubunda anlamlı olarak daha düşüktü (87,4 ±3,4'e karşılık 90,1 ±1,4, p<0,001). Çalışma grubunda ortalama kordon kanı iskemi modifiye albümin (İMA) 0,69 ±0,02 Absorbans Birimi, kordon kanı nativ tiyol (SH) düzeyi 410,2 ±80,2 ve kordon kanı total tiyol düzeyi 461,1 ±82,1 μmol/l idi. Çalışma grubunun kordon kanı örneklerinde İMA ve Native tiyol/toplam tiyol oranı dışındaki tüm parametreler kontrol grubuna göre daha yüksekti. Sonuç: Kontrol grubu ile karşılaştırıldığında idiyopatik polihidramnios ile komplike olan gebeliklerde tiyol/disülfür dengesi anti-oksidatif duruma doğru kaymıştır.

Keywords

• İMA
• tiyol/ disülfid homeostazı
• polihidramnioz

References

1. 1. Sandlin, A.T., ; Chauhan, S.P., ; Magann, E.F. Clinical Relevance of Sonographically Estimated Amniotic Fluid Volume: Polyhydramnios. J. Ultrasound Med. 2013;, 32:, 851–863.
2. 2. Bas Lando M, Urman M, Weiss Y, Srebnik N, Grisaru-Granovsky S, Farkash R, Sela HYet al. Term Idiopathic Polyhydramnios, and Labor Complications. J Clin Med. 2023 Jan 27;12(3):981.
3. 3. Erel O. Neselioglu S. A novel and automated assay for thiol/disulfide homeostasis. Clin Biochem.DOI: 10.1016/j.clinbiochem .2014;47(18):326-32.2014.09.026
4. 4. Chen W, Zhao Y, Seefeldt T, Guan X. Determination of thiols and disulfides via HPLC quantification of 5-thio-2-nitrobenzoic acid. Journal of Pharma ceutical and Biomed ical Analysis 2008; 48: 1375-1380.
5. 5. Brulisauer L, Gauthier MA, Leroux JC. Disulfide-containing parenteral delivery systems and their redox-biological fate. JJournal of Control led Releaseelease 2014; 195: 147-154.
6. 6. Sanchez-Rodriguez MA, Mendoza-Nunez VM. Oxidative stress indexes for diagnosis of health or disease in humans. Oxidative Medicine and Celllular Longev ity 2019; 2019: 4128152.
7. 7. Katar-Yildirim C, Tokmak A, Yildirim C, Erel O, et alCaglar AT. Investigation of serum thiol/disulphide homeostasis in patients with abortus imminens. The Journal Jof Matern al-Fetal & Neonat al Medicine 2017;: 1-6:. 8.
8. 8. Ergin M, Cendek BD, Neselioglu S, Avsar AF, Erel Oet al. Dynamic thiol-disulfide homeostasis in hyperemesis. JJournal of Perinat tology 2015; 35: 788-792.

9. 9. Akkaya H, Uysal G, Buke B, Gok G, Erel O et al. Evaluation of fetal serum thiol/disulphide homeostasis in deliveries complicated by nuchal cord. J Matern Fetal Neonat Med The Journal of Maternal-Fetal & Neonatal Medicine 2018: 1-5.
10. 10. Cetin O, Karaman E, Boza B, Cim N, Alisik M et al. The maternal serum thiol/disulfide homeostasis is impaired in pregnancies complicated by idiopathic intrauterine growth restriction. J Matern Fetal Neonat Med The Journal of Maternal-Fetal & Neonatal Medicine 2018; 31: 607- 613.
11. 11. Erel O,Neselioglu SS,et al.. A novel and automated assay for thiol/disulphide homeostasis. Clinnical Biochem 2014;istry 47:7 (2014) 326–332.
12. 12.D. Bar-Or, E. Lau, J. V. Winkler, “A novel assay for cobalt albumin binding and its potential as a marker for myocardial ischemia—a preliminary report,” Journal of Emergency MedicineJ Emerg Med 2000;, vol. 19(, no. 4):, pp. 311–315, 2000.
13. 13. Myatt L, Cui X. Oxidative stress in the placenta. Histochem Cell Biol.2004;122:369-382.
14. 14. Wang Y, Walsh SW. Placental mitochondria as a source of oxidative stres in preeclampsia. Placenta 1998;19:581–586.