Hass ve Fuerte Avokado Yapraklarında Farklı Ekstraksiyon Yöntemleri ile Antioksidan ve Fenolik İçeriklerin Karşılaştırmalı Analizi

Abstract

Arka Plan: Son yıllarda, avokado meyvesinin yaprak ve tohumlarında bulunan antioksidanlar ve fenolik bileşiklerin potansiyel sağlık yararları ile sürdürülebilir kullanım olanaklarına yönelik ilgi artmaktadır. Amaç: Bu çalışma, Alanya bölgesinde yetiştirilen iki farklı avokado çeşidinin yapraklarından elde edilen toplam antioksidan kapasite (TAK), toplam fenolik içerik (TFİ) ve flavonoid içerik (Fİ) miktarlarını en yüksek düzeyde sağlayan ekstraksiyon yöntemini belirlemeyi amaçlamıştır. Yöntem: Çeşitlerin TAK değerleri DPPH●, ABTS+● ve FRAP yöntemleri kullanılarak belirlenmiştir. Ekstraksiyon işlemleri için infüzyon, maserasyon, Soxhlet ve ultrason destekli ekstraksiyon yöntemleri uygulanmıştır. Bulgular: En yüksek ABTS radikal süpürücü etki, Hass çeşidinde infüzyon yöntemiyle, Fuerte çeşidinde ise Soxhlet yöntemiyle elde edilmiştir (p<0,001). FRAP sonuçları, Hass çeşidinde infüzyon ve maserasyon yöntemlerinde, Fuerte çeşidinde ise yalnızca maserasyon ekstraktlarında en yüksek düzeyde bulunmuştur (p<0,001). Her iki bitkide de en yüksek TFİ ve Fİ değerleri maserasyon, Soxhlet ve ultrason destekli ekstraktlarda saptanmıştır (p<0,001). Sonuç: Elde edilen bulgular, farklı avokado çeşitlerinin yapraklarında antioksidan kapasite ve fenolik bileşik içeriğinin ayrıntılı olarak belirlenmesi gerekliliğini ortaya koymakta ve Ar-Ge amaçlı ekstraksiyon ve ürün geliştirme çalışmalarının önemini vurgulamaktadır.

Keywords

• Avokado
• Persea americana
• Hass
• Fuerte
• antioksidan kapasite
• Fenolik içerik
• ekstraksiyon yöntemleri
• DPPH
• ABTS
• FRAP

Supporting Institution

Nuh Naci Yazgan Üniversitesi BAP

Project Number

2021/SA-BP 6

Ethical Statement

Araştırma etik onay gerektirmemektedir.

Comparative Analysis of Antioxidant and Phenolic Content in Hass and Fuerte Avocado Leaves Using Different Extraction Methods

Abstract

Background: In recent years, there has been increasing interest in the potential health benefits and sustainable utilization possibilities of antioxidants and phenolic compounds found in the leaves and seeds of avocado fruit. Aim: This study aimed to determine the method that yields the highest total antioxidant capacity (TAC), total phenolic content (TPC), and flavonoid content (TFC) from the leaves of two avocado grown in the Alanya region. Method: TAC of the species was determined using the DPPH●, ABTS+●, and FRAP methods. For the extraction process, Infusion, Maceration, Soxhlet, and Ultrasound-Assisted Extraction methods were used. Results: The highest ABTS radical scavenging effect was observed in the Hass type with the infusion method and in Fuerte with the Soxhlet method (p<0.001). FRAP results were highest in the Hass type with infusion and maceration methods and in Fuerte with only the maceration extracts (p<0.001). The highest TPC and TFC amounts in both plants were obtained with maceration, Soxhlet, and ultrasound-assisted extracts (p<0.001). Conclusion: These results underscore the necessity for detailed determination of the antioxidant capacities and phenolic compound content of different types of avocado leaves and highlight the importance of conducting further studies on extraction and product preparation for R&D purposes

Keywords

• Avocado
• Persea Americana
• Hass
• Fuerte
• antioxidant capacity
• phenolic content
• extraction method
• DPPH
• ABTS
• FRAP

Supporting Institution

Scientific Research Projects Coordination Unit of Nuh Naci Yazgan University

Project Number

2021/SA-BP 6

Ethical Statement

This research did not require ethics committee approval.

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