Hass ve Fuerte Avokado Yapraklarında Farklı Ekstraksiyon Yöntemleri ile Antioksidan ve Fenolik İçeriklerin Karşılaştırmalı Analizi

Yıl 2026, Cilt: 16 Sayı: 1, 7 - 12, 31.01.2026

Kübra Yavuz , Neriman İnanç , Leyla Paşayeva , Yusuf Aykemat

https://doi.org/10.16899/jcm.1767681

Öz

Arka Plan: Son yıllarda, avokado meyvesinin yaprak ve tohumlarında bulunan antioksidanlar ve fenolik bileşiklerin potansiyel sağlık yararları ile sürdürülebilir kullanım olanaklarına yönelik ilgi artmaktadır.

Amaç: Bu çalışma, Alanya bölgesinde yetiştirilen iki farklı avokado çeşidinin yapraklarından elde edilen toplam antioksidan kapasite (TAK), toplam fenolik içerik (TFİ) ve flavonoid içerik (Fİ) miktarlarını en yüksek düzeyde sağlayan ekstraksiyon yöntemini belirlemeyi amaçlamıştır.

Yöntem: Çeşitlerin TAK değerleri DPPH●, ABTS+● ve FRAP yöntemleri kullanılarak belirlenmiştir. Ekstraksiyon işlemleri için infüzyon, maserasyon, Soxhlet ve ultrason destekli ekstraksiyon yöntemleri uygulanmıştır.

Bulgular: En yüksek ABTS radikal süpürücü etki, Hass çeşidinde infüzyon yöntemiyle, Fuerte çeşidinde ise Soxhlet yöntemiyle elde edilmiştir (p<0,001). FRAP sonuçları, Hass çeşidinde infüzyon ve maserasyon yöntemlerinde, Fuerte çeşidinde ise yalnızca maserasyon ekstraktlarında en yüksek düzeyde bulunmuştur (p<0,001). Her iki bitkide de en yüksek TFİ ve Fİ değerleri maserasyon, Soxhlet ve ultrason destekli ekstraktlarda saptanmıştır (p<0,001).

Sonuç: Elde edilen bulgular, farklı avokado çeşitlerinin yapraklarında antioksidan kapasite ve fenolik bileşik içeriğinin ayrıntılı olarak belirlenmesi gerekliliğini ortaya koymakta ve Ar-Ge amaçlı ekstraksiyon ve ürün geliştirme çalışmalarının önemini vurgulamaktadır.

Anahtar Kelimeler

Avokado , Persea americana , Hass , Fuerte , antioksidan kapasite , Fenolik içerik , ekstraksiyon yöntemleri , DPPH , ABTS , FRAP

Etik Beyan

Araştırma etik onay gerektirmemektedir.

Destekleyen Kurum

Nuh Naci Yazgan Üniversitesi BAP

Proje Numarası

2021/SA-BP 6

Comparative Analysis of Antioxidant and Phenolic Content in Hass and Fuerte Avocado Leaves Using Different Extraction Methods

Öz

Background: In recent years, there has been increasing interest in the potential health benefits
and sustainable utilization possibilities of antioxidants and phenolic compounds found in the
leaves and seeds of avocado fruit.
Aim: This study aimed to determine the method that yields
the highest total antioxidant capacity (TAC), total phenolic content (TPC), and flavonoid
content (TFC) from the leaves of two avocado grown in the Alanya region.
Method: TAC of the species was determined using the DPPH●, ABTS+●, and FRAP methods. For the extraction
process, Infusion, Maceration, Soxhlet, and Ultrasound-Assisted Extraction methods were
used.
Results: The highest ABTS radical scavenging effect was observed in the Hass type with
the infusion method and in Fuerte with the Soxhlet method (p<0.001). FRAP results were
highest in the Hass type with infusion and maceration methods and in Fuerte with only the
maceration extracts (p<0.001). The highest TPC and TFC amounts in both plants were obtained
with maceration, Soxhlet, and ultrasound-assisted extracts (p<0.001).
Conclusion: These results underscore the necessity for detailed determination of the antioxidant capacities and
phenolic compound content of different types of avocado leaves and highlight the importance
of conducting further studies on extraction and product preparation for R&D purposes

Anahtar Kelimeler

Avocado , Persea Americana , Hass , Fuerte , antioxidant capacity , phenolic content , extraction method , DPPH , ABTS , FRAP

Etik Beyan

This research did not require ethics committee approval.

Destekleyen Kurum

Scientific Research Projects Coordination Unit of Nuh Naci Yazgan University

Proje Numarası

2021/SA-BP 6

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