Melatonin receptors increase Momordica’s anticancer effects against PC-3 and HT-29

Year 2021, Volume: 11 Issue: 2, 166 - 173, 25.03.2021

Ali Taghizadehghalehjoughi , Yeşim Yeni , Sıdıka Genç David R Wallace Ahmet Hacimuftuoglu , Zeynep Çakır

https://doi.org/10.16899/jcm.849993

Cited By: 3

Abstract

Aim: The aim of our study is to the evaluation of melatonin (MLT) and Momordica charantia (MC) combination on PC-3 and HT-29 cancer lines and to address the question of where or not MLT increases MC antitumor effect in the PC-3 and HT-29 cancer lines.
Material and Method: The PC-3 and HT-29 cell lines were grown in a manufacturer-specified culture medium. Cisplatin, MLT, increasing concentrations of MC, 40 μg/ml MLT + increasing concentrations MC were applied to PC-3 and HT-29 cell lines for 72 hours. 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) cell viability, Total Antioxidant Capacity (TAC), Total Oxidant Status (TOS), Cellular Migration (Wound Healing test), and Lactate Dehydrogenase (LDH) tests were done 72 hours after drug administration.
Results: The combination of MLT 40 μg/ml + MC 100 µg/ml reduced cell viability in both PC-3 and HT-29 cells. Besides, TAC and TOS levels showed a correlation with LDH and MTT assays and were found to be statistically significant (P<0.05). Also, it was observed in the migration test that the wound line widened in our combination groups from the 24th hours. However, it was observed that it only prevented migration at 72nd hours in pure groups.
Conclusion: The combination of 40 μg/ml MLT with MC increased the antitumor effect compared to MC alone and reduced the viability of cancer cells more effectively than MC alone. So, MLT + MC treatment combination can be a new resource of therapeutics.

Keywords

HT-29, LDH, Melatonin, Momordica, PC-3

Melatonin reseptörleri PC-3 ve HT-29'a karşı Momordica'nın antikanser etkilerini artırır

Abstract

Amaç: Çalışmamızın amacı, PC-3 ve HT-29 kanser hatlarında melatonin (MLT) ve Momordica charantia (MC) kombinasyonunun değerlendirilmesi ve MLT'nin PC-3 ve HT-29 kanser hatlarında MC antitümör etkisini nerede artırdığı sorusunu ele almaktır.
Gereç ve yöntem: PC-3 ve HT-29 hücre çizgileri, üreticinin belirlediği bir kültür ortamında büyütüldü. Sisplatin, MLT, artan MC konsantrasyonları, MLT 40 μg / ml + artan konsantrasyonlar MC, PC-3 ve HT-29 hücre hatlarına 72 saat süreyle uygulandı. 3- (4,5-Dimetiltiyazol-2-Yl) -2,5-Difeniltetrazolyum Bromür (MTT) hücre canlılığı, Toplam Antioksidan Kapasitesi (TAC), Toplam Oksidan Durumu (TOS), Hücresel Göç (Yara İyileştirme testi) ve Laktat Dehidrojenaz (LDH) testleri, ilaç uygulamasından 72 saat sonra yapıldı.
Bulgular: MLT 40 ug / ml + MC 100 ug / ml kombinasyonu, hem PC-3 hem de HT-29 hücrelerinde hücre canlılığını azalttı. Ayrıca TAC ve TOS seviyeleri LDH ve MTT testleri ile korelasyon gösterdi ve istatistiksel olarak anlamlı bulundu (P <0.05). Ayrıca migrasyon testinde kombinasyon gruplarımızda 24. saatten itibaren yara hattının genişlediği gözlendi. Ancak saf gruplarda göçü sadece 72. saatte engellediği görülmüştür.
Sonuç: 40 ug / ml MLT'nin MC ile kombinasyonu, tek başına MC'ye kıyasla antitümör etkisini arttırdı ve kanser hücrelerinin canlılığını tek başına MC'den daha etkili bir şekilde azalttı. Dolayısıyla, MLT + MC tedavi kombinasyonu yeni bir terapötik kaynak olabilir.

Keywords

HT-29, LDH, Melatonin, Momordica, PC-3

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