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Abstract

The wound is the deterioration of anatomical and functional integrity of the living tissue. Wound healing is initiation of regular and sequent cellular and biochemical events after trauma and resulting with formation of new tissues. Collagens play a very important role on successful completion of wound healing. The basic amino acids in the form of collagen, which is the maximum amount of protein in body, are proline and hydroxyproline. In this study, proline amino acid was applied as local and systemic to wounds made on the dorsal of female rats for 30 days and healing was observed. At the end of this period, samples were taken from the dorsal areas and subjected to tensile test. In conclusion, it was mechanically confirmed that proline amino acids had a positive effect on wound healing and systemic application was more successful

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DERİ İYİLEŞMESİNİN MEKANİK OLARAK İNCELEMESİ

Abstract

Yara, canlı dokunun anatomik ve fonksiyonel bütünlüğünün bozulmasıdır. Yara iyileşmesi ise travma sonrasında başlayan düzenli ve sıralı hücresel ve biyokimyasal olayların yeni doku oluşumu ile sonuçlanmasıdır. Kollajenler yara iyileşmesinin başarılı olarak tamamlanmasında çok önemli rol oynamaktadır. Vücutta en fazla miktarda bulunan protein olan kollajenin yapısında bulunan temel aminoasitler prolin ve hidroksiprolindir. Bu çalışmada dişi sıçanların sırtında açılan yaralara 30 gün boyunca prolin aminoasidi lokal ve sistemik olarak uygulanmış ve yara iyileşmesi izlenmiştir. Bu sürenin sonunda sırt bölgelerinden numuneler alınarak çekme testine tabi tutulmuştur. Sonuç olarak prolin aminoasidinin yara iyileşmesi üzerine olumlu etkisi olduğu mekanik açıdan doğrulamış ve sistemik uygulamasının daha başarılı olduğunu göstermiştir.

Keywords

• Deri

References

1. Baker, D.H., 2009. Advances in Protein-Amino Acid Nutrition of Poultry. Amino Acids, 37, 29–41.
2. Barbul, A., 2008. Proline Precursors to Sustain Mammalian Collagen Synthesis. J. Nutr., 138(10), 2021S–2024S.
3. Chojkier, M., Houglum, K., Herruzo, J.S., Brenner, D.A., 1989. Stimulation of Collagen Gene Expression by Ascorbic Acid in Cultures Human Fibroblasts: a Role for Llipid Peroxidation. J Biol Chem 264, 16957– 16962.
4. Hu, C.A., Khalil, S., Zhaorigetu, S., Liu, Z., Tyler, M., Wan, G., Velle, D., 2008. Human D1-Pyrroline-5- Carboxylate Synthase: Function and Regulation. Amino Acids 35, 665–672.
5. Hunt, T., 2004. Wound Healing. Current Surgical Diagnosis & Treatment’de. Ed: Way LW, Doherty GM. United States of America : McGraw Hill, 75-88.
6. Karsıdag, T., Assensio, J.A., Kabukcuoglu, F., Tuzun, S., 2010.Preliminary Study Somparing the Effects of Locally and Systemically Applied L-carnitine on the Healing of Full-Tthickness Skin Defects. Scand J Surg., 99(3), 147-152.
7. Ponrasu, T., Jamuna, S., Mathew, A., et al., 2013. Efficacy of L-Proline Administration on the Early Responses during Cutaneous Wound Healing in Rats. Amino Acids, 45(1), 179-898) Williams JZ, Barbul A: Nutrition and wound healing. Surg Clin North Am 83:571, 2003.
8. Townsend, C.M., Beauchamp, D.R., Evers, M.B., Mattox, K.L., 2004. Sabiston Textbook of Surgery: The Biological Basis of Modern Surgical Practice. 17th ed. Philadelphia, Elsevier Saunders.

9. Wu, G., Bazer, F.W., Burghardt, R.C. et al., 2011. Proline and Hydroxyproline Metabolism: İmplications For Animal And Human Nutrition. Amino Acids, 40, 1053– 1063.