ANTIOXIDANT AND ANTICHOLINESTERASE ACTIVITIES OF JUGLANS REGIA L. GROWING IN TURKEY

Abstract

        Objective: World's ageing population continues to grow older together with high percentage prevalence of Alzheimer's disease encourage science to find herbs or biologic compounds which can be utilized for it prophylaxis. Juglans regia L. is not simply an agricultural product, its fruits, leaves, barks, stems, pericarps, and flowers are all utilized for diversified medicinal usages. In the present investigation, we examined the anticholinesterase and antioxidant capacities of the lyophilized aqueous extracts of leaves from J. regia.

        Material and Method: Phenolic contents and antioxidant activity were assessed by Folin-Ciocalteu's, qualitative/quantitative DPPH and TBA methods. Anticholinesterase activity was assessed by Ellman’s method.

        Result and Discussion: The total phenolic content was found 478.32 mg/g. The extract indicated antioxidant activity with DPPH test (IC₅₀: 123.66 μg/mL) and TBA assay (IC₅₀: 209.23 μg/mL). Moreover, the extract of J. regia indicated remarkable inhibition towards acetylcholinesterase (40.09%) and butyrylcholinesyterase (56.23%) enzymes. These data propose that J. regia can be utilised a potency for pharmaceutical products which get antioxidant and anticholinesterase activity.

Keywords

• Antioxidant,Anticholinesterase,Juglans regia,Juglandaceae

TÜRKİYE'DE YETİŞEN JUGLANS REGIA L.’NIN ANTİOKSİDAN VE ANTİKOLİNESTERAZ AKTİVİTELERİ

Abstract

        Amaç: Dünyanın yaşlanan nüfusu, Alzheimer hastalığının yüksek oranda prevalansı ile birlikte yaşlanmaya devam etmekte ve bilimi, profilakside kullanılabilecek bitkileri veya biyolojik bileşikleri bulmaya teşvik etmektedir. Juglans regia L. basit tarımsal bir ürün değildir, meyvelerinin, yapraklarının, kabuklarının, gövdelerinin, perikarplarının ve çiçeklerinin farklı tıbbi kullanım alanları bulunmaktadır. Bu çalışmada, J. regia'nın yapraklarının liyofilize sulu ekstrelerinin antikolinesteraz ve antioksidan kapasitelerini inceledik.

        Gereç ve Yöntem: Fenolik içerik ve antioksidan aktivite Folin-Ciocalteu, kalitatif/kantitatif DPPH ve TBA yöntemleri ile belirlenmiştir. Antikolinesteraz aktivite ise Ellman metodu ile değerlendirilmiştir.

        Sonuç ve Tartışma: Total fenolik içerik 478.32 mg/g olarak bulunmuştur. Örnekler DPPH (123.66 μg/mL) ve TBA (209.23 μg/mL) testleri ile antioksidan aktivite göstermiştir. Ayrıca, ceviz ekstresi, sırasıyla asetilkolinesteraz (%40.09) ve butirilkolinesteraz (%56.23) enzimlerine karşı önemli ölçüde inhibisyon göstermiştir. Bu veriler, J. regia'nın, antioksidan ve antikolinesteraz aktivitesi alan farmasötik ürünler için bir potansiyel olarak kullanabileceğini önermektedir.       

Keywords

• Antioksidan,Antikolinesteraz,Juglans regia,Juglandaceae

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