ANTIOXIDANT AND ANTICHOLINESTERASE ACTIVITIES OF JUGLANS REGIA L. GROWING IN TURKEY

Yıl 2019, , 230 - 238, 08.09.2019

Songül Karakaya , Mehmet Koca Fatma Yeşilyurt , Ahmet Hacımüftüoğlu

https://doi.org/10.33483/jfpau.530099

Cited By: 3

Öz

       
Objective: World's ageing population continues to grow older
together with high percentage prevalence of Alzheimer's disease encourage
science to find herbs or biologic compounds which can be utilized for it
prophylaxis. Juglans regia L. is not simply an agricultural product, its
fruits, leaves, barks, stems, pericarps, and flowers are all utilized for
diversified medicinal usages. In the present investigation, we examined the
anticholinesterase and antioxidant capacities of the lyophilized aqueous
extracts of leaves from J. regia.

        Material and Method: Phenolic contents and antioxidant
activity were assessed by Folin-Ciocalteu's, qualitative/quantitative DPPH and
TBA methods. Anticholinesterase activity was assessed by Ellman’s method.

        Result and Discussion: The total phenolic content was
found 478.32 mg/g. The extract indicated antioxidant activity with DPPH test
(IC₅₀: 123.66 μg/mL) and
TBA assay (IC₅₀: 209.23 μg/mL).
Moreover, the extract of J. regia indicated remarkable inhibition towards
acetylcholinesterase (40.09%) and butyrylcholinesyterase (56.23%) enzymes.
These data propose that J. regia can be utilised a potency for pharmaceutical
products which get antioxidant and anticholinesterase activity.

Anahtar Kelimeler

Antioxidant, Anticholinesterase, Juglans regia, Juglandaceae

TÜRKİYE'DE YETİŞEN JUGLANS REGIA L.’NIN ANTİOKSİDAN VE ANTİKOLİNESTERAZ AKTİVİTELERİ

Öz

        Amaç: Dünyanın
yaşlanan nüfusu, Alzheimer hastalığının yüksek oranda prevalansı ile birlikte
yaşlanmaya devam etmekte ve bilimi, profilakside kullanılabilecek bitkileri
veya biyolojik bileşikleri bulmaya teşvik etmektedir. Juglans regia L. basit
tarımsal bir ürün değildir, meyvelerinin, yapraklarının, kabuklarının,
gövdelerinin, perikarplarının ve çiçeklerinin farklı tıbbi kullanım alanları
bulunmaktadır. Bu çalışmada, J. regia'nın yapraklarının liyofilize sulu
ekstrelerinin antikolinesteraz ve antioksidan kapasitelerini inceledik.

       
Gereç ve Yöntem: Fenolik
içerik ve antioksidan aktivite Folin-Ciocalteu, kalitatif/kantitatif DPPH ve
TBA yöntemleri ile belirlenmiştir. Antikolinesteraz aktivite ise Ellman metodu
ile değerlendirilmiştir.

        Sonuç ve Tartışma: Total fenolik içerik 478.32 mg/g olarak
bulunmuştur. Örnekler DPPH (123.66 μg/mL) ve TBA (209.23 μg/mL) testleri ile
antioksidan aktivite göstermiştir. Ayrıca, ceviz ekstresi, sırasıyla
asetilkolinesteraz (%40.09) ve butirilkolinesteraz (%56.23) enzimlerine karşı
önemli ölçüde inhibisyon göstermiştir. Bu veriler, J. regia'nın, antioksidan ve
antikolinesteraz aktivitesi alan farmasötik ürünler için bir potansiyel olarak
kullanabileceğini önermektedir.       

Anahtar Kelimeler

Antioksidan, Antikolinesteraz, Juglans regia, Juglandaceae

Kaynakça

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