Comparative Evaluation of Antioxidant Capacities of Sage (Salvia spp.) Extracts Using Different Solvents

Year 2025, Volume: 10 Issue: 4, 72 - 79, 31.12.2025

Nurdan Gökduman , Şaban Kalay

https://doi.org/10.58854/jicm.1784684

https://izlik.org/JA67HP37MP

Abstract

Backround: Growing interest in natural approaches to disease prevention has led to an increased demand for systematic and reliable information in herbal medicine. Oxidative stress has been linked to numerous diseases, and antioxidants are becoming increasingly important in reducing oxidative damage. The genus Salvia, belonging to the Lamiaceae family, is known for its antioxidant properties. In this study, the antioxidant capacities of sage (Salvia spp.), a plant commonly consumed as tea or used as a gargle and also containing endemic species in Anatolia were investigated using different solvents: ethanol, methanol, chloroform, and water.
Materials and Methods: The extracts were prepared by maceration, and their antioxidant activities were determined based on the percentage inhibition of the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation. The primary aim of the study was to identify the extract with the highest antioxidant activity. In addition, the antioxidant capacities of the extracts were compared to butylated hydroxytoluene (BHT), a commonly used synthetic antioxidant.
Results: The water, methanol, and ethanol extracts exhibited the highest antioxidant activities, all of which were found to be higher than that of BHT. In contrast, the chloroform extract showed lower antioxidant capacity compared to both the other extracts and BHT.
Conclusions: These findings concluded that sage extracts obtained with polar solvents may serve as effective natural antioxidant agents.

Keywords

Salvia spp , Antioxidant activity , ABTS assay , Maceration , BHT

Ethical Statement

Not applicable

Supporting Institution

TÜBİTAK 2209-A Project. Project Title: Investigation of the Antioxidant Capacities of Different Extracts of Sage (Salvia spp.).

Project Number

TÜBİTAK 2209-A Project. Project Title: Investigation of the Antioxidant Capacities of Different Extracts of Sage (Salvia spp.).

Thanks

The author is very thankful to University of Health Sciences for their contribution to the completion of this research.

Adaçayı (Salvia spp.) Bitki Ekstrelerinin Farklı Çözücüler Kullanılarak Antioksidan Kapasitelerinin Karşılaştırmalı Olarak Değerlendirilmesi

https://izlik.org/JA67HP37MP

Abstract

Backround: Hastalıklardan korunmanın farklı yollarının araştırılmasıyla günümüzde bitkisel tedavilerde sistemik ve güvenilir bilgiye gereksinim artmıştır. Oksidatif stres birçok hastalık ile ilişkili bulunmuş ve oksidatif stresin azaltılmasında antioksidanlar giderek önem kazanmıştır. Lamiaceae familyasına ait Salvia cinsinin antioksidan özellikleri bilinmektedir. Bu çalışmada Anadolu’da endemik türleri de bulunan, halk arasında genellikle çay ve gargara olarak kullanılan adaçayı (Salvia spp.) bitkisinin etanol, metanol ve kloroform ve çözücüleri ile hazırlanan ekstrelerinin antioksidan kapasiteleri incelenmiştir. Böylece en yüksek antioksidan aktiviteye sahip adaçayı ekstresinin belirlenmesi amaçlanmıştır.
Materials and Methods: Maserasyon yöntemiyle elde edilen ekstrelerin, 2,2-azinobis (3-etilbenzothiazollin-6-sulfonik asit) (ABTS) radikal katyonunun inhibisyon yüzdesine göre antioksidan aktiviteleri belirlenmiştir. Ayrıca ekstrelerin antioksidan aktiviteleri, sentetik antioksidan olarak kullanılan bütil hidroksitolüen (BHT) ile karşılaştırılmıştır.
Results: Sırasıyla su, metanol ve etanol ekstreleri en yüksek antioksidan aktiviteleri gösterirken, bu aktiviteler BHT’ye kıyasla daha yüksek bulunmuştur. Öte yandan, kloroform ekstresi diğer çözücülere ve BHT’ye göre daha düşük antioksidan kapasite göstermiştir.
Conclusions: Bu bulgular, polar çözücülerle elde edilen adaçayı özlerinin etkili doğal antioksidan ajanlar olarak hizmet edebileceğini ortaya koymuştur.

Keywords

Adaçayı , Antioksidan aktivite , ABTS , Maserasyon , BHT

Ethical Statement

Gerekli değil

Supporting Institution

TÜBİTAK 2209-A Proje başlıği: Ada Çayının (Salvia spp.) Farklı Ekstrelerinin Antioksidan Kapasitelerinin Araştırılması

Project Number

TÜBİTAK 2209-A Project. Project Title: Investigation of the Antioxidant Capacities of Different Extracts of Sage (Salvia spp.).

Thanks

Yazar, bu araştırmanın tamamlanmasına katkılarından dolayı Sağlık Bilimleri Üniversitesi’ne teşekkürlerini sunar.

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