Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq

Year 2018, , 15 - 22, 01.06.2018

Hasan Raheem Khudhur , Ghada Basil Alomashi

https://doi.org/10.5455/jicm.30.20180422

Abstract

Background: Cutaneous
leishmaniasis (CL) is vector-borne disease. and endemics in most regions of
Iraq especially with poor populations. "Natural resistance-associated macrophage protein 1 (NRAMP1)" gene play an essential
role in susceptibility to CL and disease pathology, NRAMP1 influences a production
and activation of pro-inflammatory cytokines like IL-1β and TNF-α. Pro- and
anti-inflammatory cytokines play an essential role in susceptibility/resistance
and the immunopathogenesis of Leishmania infection. These cytokines are crucial
factors in the initiation and enhances of protective immunity against
Leishmania infection. This study aimed to determine effect of polymorphism in NRAMP1 genes on cytokines secretion and
their effect in susceptibility to CL infection.

Materials and Methods: Samples of blood were collected from patients (n: 60) with
CL and apparently healthy controls (n: 32). Polymorphism of Nramp1 (D543N) detected by PCR-RFLP technique in patients and control
groups while (TNF-α and IL-1β) cytokine concentrations detected by ELISA
technique using a quantitative sandwich enzyme immunoassay technique.

Results: Results
indicate to effect of Nramp1 gene polymorphism
on levels of (IL-1β and TNF-α) cytokines and this a clearly recorded in present
study were A allele is associated with lower levels of (TNF-α and IL-1β) in
patients and control groups compression to that absorbed in allele G with
statically significant (p ≤ 0.05).

Conclusions: Cytokines
(IL-1β and TNF-α) plays an essential 
role in the resolution of CL infection, 
were its concentration  in
patients serum of all age groups were significant increase in comparison to
that observed in their control groups. In polymorphisms of Nramp1 (D543N) gene, were
A allele is associated with lower levels of (IL-1β and TNF-α) compression to
that  absorbed in allele G, and this
decreased production may be associated with susceptibility and proliferation of
parasites in the macrophage.

Keywords

Cutaneous leishmaniosis, NRAMP1polymorphisms, TNF-α and IL-1β, Cytokine

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