Research Article
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Year 2018, , 15 - 22, 01.06.2018
https://doi.org/10.5455/jicm.30.20180422

Abstract

References

  • 1. Ahmed S, Colmenares M, Soong L, Goldsmith-Pestana K, Munstermann L, Molina R, et al. Intradermal infection model for pathogenesis and vaccine studies of murine visceral leishmaniasis. Infection and immunity. 2003;71(1):401-10.
  • 2. Blum J, Desjeux P, Schwartz E, Beck B, Hatz C. Treatment of cutaneous leishmaniasis among travellers. Journal of Antimicrobial Chemotherapy. 2004;53(2):158-66.
  • 3. Tripathi P, Singh V, Naik S. Immune response to leishmania: paradox rather than paradigm. FEMS Immunology & Medical Microbiology. 2007;51(2):229-42.
  • 4. Vidal S, Tremblay ML, Govoni G, Gauthier S, Sebastiani G, Malo D, et al. The Ity/Lsh/Bcg locus: natural resistance to infection with intracellular parasites is abrogated by disruption of the Nramp1 gene. Journal of Experimental Medicine. 1995;182(3):655-66.
  • 5. Mulero V, Searle S, Jenefer M, Brock JH. Solute carrier 11a1 (Slc11a1; formerly Nramp1) regulates metabolism and release of iron acquired by phagocytic, but not transferrin-receptor-mediated, iron uptake. Biochemical Journal. 2002;363(1):89-94.
  • 6. Mackenzie B, Hediger MA. SLC11 family of H+-coupled metal-ion transporters NRAMP1 and DMT1. Pflügers Archiv. 2004;447(5):571-9.
  • 7. Courville P, Chaloupka R, Cellier M. Recent progress in structure–function analyses of Nramp proton-dependent metal-ion transporters This paper is one of a selection of papers published in this Special Issue, entitled CSBMCB—Membrane Proteins in Health and Disease. Biochemistry and Cell Biology. 2006;84(6):960-78.
  • 8. Taype C, Castro J, Accinelli R, Herrera-Velit P, Shaw M, Espinoza J. Association between SLC11A1 polymorphisms and susceptibility to different clinical forms of tuberculosis in the Peruvian population. Infection, Genetics and Evolution. 2006;6(5):361-7.
  • 9. Blackwell JM, Searle S, Goswami T, Miller EN. Understanding the multiple functions of Nramp1. Microbes and infection. 2000;2(3):317-21.
  • 10. Ganz T. Iron in innate immunity: starve the invaders. Current opinion in immunology. 2009;21(1):63-7.
  • 11. Belosevic M, Davis C, Meltzer M, Nacy C. Regulation of activated macrophage antimicrobial activities. Identification of lymphokines that cooperate with IFN-gamma for induction of resistance to infection. The Journal of Immunology. 1988;141(3):890-6.
  • 12. Awasthi A, Mathur RK, Saha B. Immune response to Leishmania infection. Indian Journal of Medical Research. 2004;119(6):238.
  • 13. Santos D, Campos TM, Saldanha M, Oliveira SC, Nascimento M, Zamboni DS, et al. IL-1β production by intermediate monocytes is associated with immunopathology in cutaneous leishmaniasis. Journal of Investigative Dermatology. 2017.
  • 14. Da-Cruz AM, Bittar R, Mattos M, Oliveira-Neto MP, Nogueira R, Pinho-Ribeiro V, et al. T-cell-mediated immune responses in patients with cutaneous or mucosal leishmaniasis: long-term evaluation after therapy. Clinical and diagnostic laboratory immunology. 2002;9(2):251-6.
  • 15. Team RC. R Foundation for Statistical Computing; Vienna, Austria: 2014. R: A language and environment for statistical computing. 2015:2013.
  • 16. Al-Aubaidi IK. Serum Cytokine Production in Patients with Cutaneous Leishmaniasis Before and After Treatment. Iraqi Journal of Medical Sciences. 2011;9(1).
  • 17. Kocyigit A, Gur S, Gurel MS, Bulut V, Ulukanligil M. Antimonial therapy induces circulating proinflammatory cytokines in patients with cutaneous leishmaniasis. Infection and immunity. 2002;70(12):6589-91.
  • 18. Roberts WL, Rainey PM. Antileishmanial activity of sodium stibogluconate fractions. Antimicrobial Agents and Chemotherapy. 1993;37(9):1842-6.
  • 19. Sodhi A, Pai K, Singh RK, Singh SM. Activation of human NK cells and monocytes with cisplatin in vitro. International journal of immunopharmacology. 1990;12(8):893-8.
  • 20. Beebe AM, Mauze S, Schork NJ, Coffman RL. Serial backcross mapping of multiple loci associated with resistance to Leishmania major in mice. Immunity. 1997;6(5):551-7.
  • 21. García M, Monzote L, Montalvo AM, Scull R. Screening of medicinal plants against Leishmania amazonensis. Pharmaceutical biology. 2010;48(9):1053-8.
  • 22. Murray H, Oca M, Granger A, Schreiber R. Requirement for T cells and effect of lymphokines in successful chemotherapy for an intracellular infection. Experimental visceral leishmaniasis. Journal of Clinical Investigation. 1989;83(4):1253.
  • 23. Rais S, Perianin A, Lenoir M, Sadak A, Rivollet D, Paul M, et al. Sodium stibogluconate (Pentostam) potentiates oxidant production in murine visceral leishmaniasis and in human blood. Antimicrobial agents and chemotherapy. 2000;44(9):2406-10.
  • 24. Canonne-Hergaux F, Calafat J, Richer E, Cellier M, Grinstein S, Borregaard N, et al. Expression and subcellular localization of NRAMP1 in human neutrophil granules. Blood. 2002;100(1):268-75.
  • 25. Kim J, Lee S, Lee S, Sin C, Shim J, In K, et al. NRAMP1 genetic polymorphisms as a risk factor of tuberculous pleurisy. The International Journal of Tuberculosis and Lung Disease. 2003;7(4):370-5.
  • 26. Blackwell JM, Searle S, Mohamed H, White JK. Divalent cation transport and susceptibility to infectious and autoimmune disease: continuation of the Ity/Lsh/Bcg/Nramp1/Slc11a1 gene story. Immunology letters. 2003;85(2):197-203.
  • 27. Fritsche G, Nairz M, Werner ER, Barton HC, Weiss G. Nramp1‐functionality increases iNOS expression via repression of IL‐10 formation. European journal of immunology. 2008;38(11):3060-7.
  • 28. Nugraha J, Anggraini R. NRAMP1 polymorphism and susceptibility to lung tuberculosis in Surabaya, Indonesia. Southeast Asian Journal of Tropical Medicine and Public Health. 2011;42(2):338.
  • 29. Wessling-Resnick M. Nramp1 and other transporters involved in metal withholding during infection. Journal of Biological Chemistry. 2015;290(31):18984-90.

Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq

Year 2018, , 15 - 22, 01.06.2018
https://doi.org/10.5455/jicm.30.20180422

Abstract

Background: Cutaneous
leishmaniasis (CL) is vector-borne disease. and endemics in most regions of
Iraq especially with poor populations. "Natural resistance-associated macrophage protein 1 (NRAMP1)" gene play an essential
role in susceptibility to CL and disease pathology, NRAMP1 influences a production
and activation of pro-inflammatory cytokines like IL-1β and TNF-α. Pro- and
anti-inflammatory cytokines play an essential role in susceptibility/resistance
and the immunopathogenesis of Leishmania infection. These cytokines are crucial
factors in the initiation and enhances of protective immunity against
Leishmania infection. This study aimed to determine effect of polymorphism in NRAMP1 genes on cytokines secretion and
their effect in susceptibility to CL infection.

Materials and Methods: Samples of blood were collected from patients (n: 60) with
CL and apparently healthy controls (n: 32). Polymorphism of Nramp1 (D543N) detected by PCR-RFLP technique in patients and control
groups while (TNF-α and IL-1β) cytokine concentrations detected by ELISA
technique using a quantitative sandwich enzyme immunoassay technique.

Results: Results
indicate to effect of Nramp1 gene polymorphism
on levels of (IL-1β and TNF-α) cytokines and this a clearly recorded in present
study were A allele is associated with lower levels of (TNF-α and IL-1β) in
patients and control groups compression to that absorbed in allele G with
statically significant (p ≤ 0.05).

Conclusions: Cytokines
(IL-1β and TNF-α) plays an essential 
role in the resolution of CL infection, 
were its concentration  in
patients serum of all age groups were significant increase in comparison to
that observed in their control groups. In polymorphisms of Nramp1 (D543N) gene, were
A allele is associated with lower levels of (IL-1β and TNF-α) compression to
that  absorbed in allele G, and this
decreased production may be associated with susceptibility and proliferation of
parasites in the macrophage.

References

  • 1. Ahmed S, Colmenares M, Soong L, Goldsmith-Pestana K, Munstermann L, Molina R, et al. Intradermal infection model for pathogenesis and vaccine studies of murine visceral leishmaniasis. Infection and immunity. 2003;71(1):401-10.
  • 2. Blum J, Desjeux P, Schwartz E, Beck B, Hatz C. Treatment of cutaneous leishmaniasis among travellers. Journal of Antimicrobial Chemotherapy. 2004;53(2):158-66.
  • 3. Tripathi P, Singh V, Naik S. Immune response to leishmania: paradox rather than paradigm. FEMS Immunology & Medical Microbiology. 2007;51(2):229-42.
  • 4. Vidal S, Tremblay ML, Govoni G, Gauthier S, Sebastiani G, Malo D, et al. The Ity/Lsh/Bcg locus: natural resistance to infection with intracellular parasites is abrogated by disruption of the Nramp1 gene. Journal of Experimental Medicine. 1995;182(3):655-66.
  • 5. Mulero V, Searle S, Jenefer M, Brock JH. Solute carrier 11a1 (Slc11a1; formerly Nramp1) regulates metabolism and release of iron acquired by phagocytic, but not transferrin-receptor-mediated, iron uptake. Biochemical Journal. 2002;363(1):89-94.
  • 6. Mackenzie B, Hediger MA. SLC11 family of H+-coupled metal-ion transporters NRAMP1 and DMT1. Pflügers Archiv. 2004;447(5):571-9.
  • 7. Courville P, Chaloupka R, Cellier M. Recent progress in structure–function analyses of Nramp proton-dependent metal-ion transporters This paper is one of a selection of papers published in this Special Issue, entitled CSBMCB—Membrane Proteins in Health and Disease. Biochemistry and Cell Biology. 2006;84(6):960-78.
  • 8. Taype C, Castro J, Accinelli R, Herrera-Velit P, Shaw M, Espinoza J. Association between SLC11A1 polymorphisms and susceptibility to different clinical forms of tuberculosis in the Peruvian population. Infection, Genetics and Evolution. 2006;6(5):361-7.
  • 9. Blackwell JM, Searle S, Goswami T, Miller EN. Understanding the multiple functions of Nramp1. Microbes and infection. 2000;2(3):317-21.
  • 10. Ganz T. Iron in innate immunity: starve the invaders. Current opinion in immunology. 2009;21(1):63-7.
  • 11. Belosevic M, Davis C, Meltzer M, Nacy C. Regulation of activated macrophage antimicrobial activities. Identification of lymphokines that cooperate with IFN-gamma for induction of resistance to infection. The Journal of Immunology. 1988;141(3):890-6.
  • 12. Awasthi A, Mathur RK, Saha B. Immune response to Leishmania infection. Indian Journal of Medical Research. 2004;119(6):238.
  • 13. Santos D, Campos TM, Saldanha M, Oliveira SC, Nascimento M, Zamboni DS, et al. IL-1β production by intermediate monocytes is associated with immunopathology in cutaneous leishmaniasis. Journal of Investigative Dermatology. 2017.
  • 14. Da-Cruz AM, Bittar R, Mattos M, Oliveira-Neto MP, Nogueira R, Pinho-Ribeiro V, et al. T-cell-mediated immune responses in patients with cutaneous or mucosal leishmaniasis: long-term evaluation after therapy. Clinical and diagnostic laboratory immunology. 2002;9(2):251-6.
  • 15. Team RC. R Foundation for Statistical Computing; Vienna, Austria: 2014. R: A language and environment for statistical computing. 2015:2013.
  • 16. Al-Aubaidi IK. Serum Cytokine Production in Patients with Cutaneous Leishmaniasis Before and After Treatment. Iraqi Journal of Medical Sciences. 2011;9(1).
  • 17. Kocyigit A, Gur S, Gurel MS, Bulut V, Ulukanligil M. Antimonial therapy induces circulating proinflammatory cytokines in patients with cutaneous leishmaniasis. Infection and immunity. 2002;70(12):6589-91.
  • 18. Roberts WL, Rainey PM. Antileishmanial activity of sodium stibogluconate fractions. Antimicrobial Agents and Chemotherapy. 1993;37(9):1842-6.
  • 19. Sodhi A, Pai K, Singh RK, Singh SM. Activation of human NK cells and monocytes with cisplatin in vitro. International journal of immunopharmacology. 1990;12(8):893-8.
  • 20. Beebe AM, Mauze S, Schork NJ, Coffman RL. Serial backcross mapping of multiple loci associated with resistance to Leishmania major in mice. Immunity. 1997;6(5):551-7.
  • 21. García M, Monzote L, Montalvo AM, Scull R. Screening of medicinal plants against Leishmania amazonensis. Pharmaceutical biology. 2010;48(9):1053-8.
  • 22. Murray H, Oca M, Granger A, Schreiber R. Requirement for T cells and effect of lymphokines in successful chemotherapy for an intracellular infection. Experimental visceral leishmaniasis. Journal of Clinical Investigation. 1989;83(4):1253.
  • 23. Rais S, Perianin A, Lenoir M, Sadak A, Rivollet D, Paul M, et al. Sodium stibogluconate (Pentostam) potentiates oxidant production in murine visceral leishmaniasis and in human blood. Antimicrobial agents and chemotherapy. 2000;44(9):2406-10.
  • 24. Canonne-Hergaux F, Calafat J, Richer E, Cellier M, Grinstein S, Borregaard N, et al. Expression and subcellular localization of NRAMP1 in human neutrophil granules. Blood. 2002;100(1):268-75.
  • 25. Kim J, Lee S, Lee S, Sin C, Shim J, In K, et al. NRAMP1 genetic polymorphisms as a risk factor of tuberculous pleurisy. The International Journal of Tuberculosis and Lung Disease. 2003;7(4):370-5.
  • 26. Blackwell JM, Searle S, Mohamed H, White JK. Divalent cation transport and susceptibility to infectious and autoimmune disease: continuation of the Ity/Lsh/Bcg/Nramp1/Slc11a1 gene story. Immunology letters. 2003;85(2):197-203.
  • 27. Fritsche G, Nairz M, Werner ER, Barton HC, Weiss G. Nramp1‐functionality increases iNOS expression via repression of IL‐10 formation. European journal of immunology. 2008;38(11):3060-7.
  • 28. Nugraha J, Anggraini R. NRAMP1 polymorphism and susceptibility to lung tuberculosis in Surabaya, Indonesia. Southeast Asian Journal of Tropical Medicine and Public Health. 2011;42(2):338.
  • 29. Wessling-Resnick M. Nramp1 and other transporters involved in metal withholding during infection. Journal of Biological Chemistry. 2015;290(31):18984-90.
There are 29 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Research Articles
Authors

Hasan Raheem Khudhur

Ghada Basil Alomashi This is me

Publication Date June 1, 2018
Published in Issue Year 2018

Cite

APA Khudhur, H. R., & Alomashi, G. B. (2018). Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq. Journal of Immunology and Clinical Microbiology, 3(2), 15-22. https://doi.org/10.5455/jicm.30.20180422
AMA Khudhur HR, Alomashi GB. Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq. J Immunol Clin Microbiol. June 2018;3(2):15-22. doi:10.5455/jicm.30.20180422
Chicago Khudhur, Hasan Raheem, and Ghada Basil Alomashi. “Effect of NRAMP1 Gene Polymorphism on Levels of (TNF-α1and IL-1β) Cytokines in Cutaneous Leishmaniasis Patients in Iraq”. Journal of Immunology and Clinical Microbiology 3, no. 2 (June 2018): 15-22. https://doi.org/10.5455/jicm.30.20180422.
EndNote Khudhur HR, Alomashi GB (June 1, 2018) Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq. Journal of Immunology and Clinical Microbiology 3 2 15–22.
IEEE H. R. Khudhur and G. B. Alomashi, “Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq”, J Immunol Clin Microbiol, vol. 3, no. 2, pp. 15–22, 2018, doi: 10.5455/jicm.30.20180422.
ISNAD Khudhur, Hasan Raheem - Alomashi, Ghada Basil. “Effect of NRAMP1 Gene Polymorphism on Levels of (TNF-α1and IL-1β) Cytokines in Cutaneous Leishmaniasis Patients in Iraq”. Journal of Immunology and Clinical Microbiology 3/2 (June 2018), 15-22. https://doi.org/10.5455/jicm.30.20180422.
JAMA Khudhur HR, Alomashi GB. Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq. J Immunol Clin Microbiol. 2018;3:15–22.
MLA Khudhur, Hasan Raheem and Ghada Basil Alomashi. “Effect of NRAMP1 Gene Polymorphism on Levels of (TNF-α1and IL-1β) Cytokines in Cutaneous Leishmaniasis Patients in Iraq”. Journal of Immunology and Clinical Microbiology, vol. 3, no. 2, 2018, pp. 15-22, doi:10.5455/jicm.30.20180422.
Vancouver Khudhur HR, Alomashi GB. Effect of NRAMP1 gene polymorphism on levels of (TNF-α1and IL-1β) cytokines in cutaneous Leishmaniasis patients in Iraq. J Immunol Clin Microbiol. 2018;3(2):15-22.

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