Year 2018, Volume , Issue , Pages 68 - 75 2018-06-05

Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection

Fariha Akhter Chowdhury, et al. [1]


Background: Urinary tract infection (UTI) is frequently encountered by female population where the episode of occurrence increases with advancing age. Escherichia coli, a common UTI causing organism, retains glutathione defense mechanisms that may allow the organism to withstand host oxidative immune response as well as the harsh physiological environment of urinary tract. The aim of this study was to analyze glutathione reductase (GR) activity of UTI causing E. coli under stressful condition. Material and Methods: E. coli isolates from urine samples of UTI female patients of different ages were sampled. Samples of isolated E. coli were grown in conditions with and without oxidative stress where the stressful condition was induced by hydrogen peroxide (H2O2). In both conditions measurement of reduced glutathione level of the isolates was performed calorimetrically using microplate reader assay. Results: Samples from elderly patients showed that GSH level significantly altered, the value of GSH (mM/mg) are 5.01±0.48 and 5.20±0.64 in normal and under stressful condition respectively, which showed increase of GSH level by 3.83% (p = 0.024) under oxidative stress condition. On the other hand, in case of adult patients, GSH (mM/mg) level found to be decreased by 5.11% (p = 0.011) with the values of 5.08±0.1 and 4.82±0.18 respectively for normal and stressed condition. Conclusion: Our data suggesting an association of GR activity with patient’s age, may signify that E. coli isolates respond differently to oxidative stressful environment in different age-related physiological changes and could help to uncover ways to gain better insight into E. coli pathogenesis of UTI predominance in aged population.

Aged, Escherichia coli, glutathione reductase, oxidative stress, reduced glutathione (GSH), Urinary tract infections (UTI)
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Primary Language en
Subjects Medicine
Journal Section Research Articles
Authors

Author: Fariha Akhter Chowdhury, et al.

Dates

Publication Date : June 5, 2018

Bibtex @research article { jicm422129, journal = {Journal of Immunology and Clinical Microbiology}, issn = {}, eissn = {2528-9470}, address = {Adalet mh. 1643/18 Sk. bina no:6 smyrnapark evleri b blok daire 16, Bayraklı, İzmir}, publisher = {Erkan YULA}, year = {2018}, volume = {}, pages = {68 - 75}, doi = {10.5455/jicm.27.20171014}, title = {Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection}, key = {cite}, author = {Chowdhury, et al., Fariha Akhter} }
APA Chowdhury, et al., F . (2018). Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection. Journal of Immunology and Clinical Microbiology , () , 68-75 . DOI: 10.5455/jicm.27.20171014
MLA Chowdhury, et al., F . "Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection". Journal of Immunology and Clinical Microbiology (2018 ): 68-75 <https://dergipark.org.tr/en/pub/jicm/issue/36924/422129>
Chicago Chowdhury, et al., F . "Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection". Journal of Immunology and Clinical Microbiology (2018 ): 68-75
RIS TY - JOUR T1 - Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection AU - Fariha Akhter Chowdhury, et al. Y1 - 2018 PY - 2018 N1 - doi: 10.5455/jicm.27.20171014 DO - 10.5455/jicm.27.20171014 T2 - Journal of Immunology and Clinical Microbiology JF - Journal JO - JOR SP - 68 EP - 75 VL - IS - SN - -2528-9470 M3 - doi: 10.5455/jicm.27.20171014 UR - https://doi.org/10.5455/jicm.27.20171014 Y2 - 2019 ER -
EndNote %0 Journal of Immunology and Clinical Microbiology Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection %A Fariha Akhter Chowdhury, et al. %T Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection %D 2018 %J Journal of Immunology and Clinical Microbiology %P -2528-9470 %V %N %R doi: 10.5455/jicm.27.20171014 %U 10.5455/jicm.27.20171014
ISNAD Chowdhury, et al., Fariha Akhter . "Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection". Journal of Immunology and Clinical Microbiology / (June 2018): 68-75 . https://doi.org/10.5455/jicm.27.20171014
AMA Chowdhury, et al. F . Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection. J Immunol Clin Microbiol. 2018; 68-75.
Vancouver Chowdhury, et al. F . Effect of oxidative stress on glutathione reductase activity of Escherichia coli clinical isolates from patients with urinary tract infection. Journal of Immunology and Clinical Microbiology. 2018; 75-68.