Determination of Genetic Variation in Some Teff Genotypes Using RAPD Markers

Abstract

As a result of increasing consumer awareness, the demand for healthy food products is increasing day by day. Teff, which has chemical composition and nutritional properties, is a plant that has been used in human nutrition since the past, and it is one of the sources that can be grown as an alternative in our country. Being a very important grain for a healthy life, teff has an important place in the diet of celiac patients because it is gluten-free. Besides, the fact that the growing conditions are not difficult and the harvest period is short, the thought that it can easily adapt to drought in the future increases the value of teff grain day by day. Within the scope of the study, 19 RAPD markers were screened using Et 2016 genotip. Genetic variation research was conducted in Teff plant with 10 RAPD primers giving good amplicon. Presence (1) and absence (0) scoring were performed by separating amplicons using agarose gel electrophoresis method after PCR with 10 RAPD primers used. By using the Jaccard similarity index with the data obtained, a dendogram was created between the genotypes with the basic coordinate analysis and Bayesian statistics. As a result of the analysis, it was determined that the genetic variation between the genotypes was low. Very little difference was detected in Zobel Basic and Dega Basic genotypes and needs to be confirmed with more markers. As a result, it is recommended to increase the variation by using different approaches (mutagens, UV, etc.) to increase the variation among these genotypes.

Keywords

• Eragrostis tef
• Molecular Markers
• Diversity

Bazı Tef Genotiplerinde RAPD Markırları Kullanılarak Genetik Varyasyonun Belirlenmesi

Abstract

Günümüzde tüketici bilincinin artması sonucu sağlıklı gıda ürünlerine talep her geçen gün artmaktadır. Kimyasal bileşimi ve beslenme özelliklere sahip olan Tef bitkisi, insan beslenmesinde geçmişten beri kullanılan bir bitki olmakla beraber, ülkemizde de alternatif olarak yetiştirilebilecek kaynaklardan biridir. Sağlıklı yaşam açısından son derece önemli bir tahıl olan tef, glutensiz olması nedeniyle de özellikle çölyak hastalarının beslenmesinde önemli yer tutar. Bunun yanında yetişme koşullarının zor olmaması ve hasat süresinin kısa olmasıyla beraber gelecekte kuraklığa kolayca uyum sağlayabileceği düşüncesi tef tahılının değerini her geçen gün arttırmaktadır. Çalışma kapsamında Et 2016 tef bitkisi kullanılarak 19 RAPD markırı ile taranmıştır. İyi amplikon veren 10 tane RAPD primeri ile Tef bitkisinde genetik varyasyon araştırması yapılmıştır. Kullanılan 10 tane RAPD primeri ile PZR sonrası agaroz jel elektroforez yöntemi ile amplikonların ayrıştırılması sağlanarak varlık (1) ve yokluk (0) skorlaması gerçekleştirilmiştir. Elde edilen verilere ile Jaccard benzerlik indeksi kullanılarak temel koordinat analizi ve Bayesian istatistiği ile genotipler arasında dendogram oluşturulmuştur. Yapılan analizler sonucunda genotipler arasındaki genetik varyasyonun düşük olduğu saptanmıştır. Zobel Basic ve Dega Basic genotiplerinde çok az düzeyde farklılık tespit edilmiş olup, daha çok markır ile teyit edilmesi gerekmektedir. Sonuç olarak bu genotipler arasındaki varyasyonun artırılması için farklı yaklaşımlar kullanılarak (mutajenler vb.) varyasyonun artırılması önerilmektedir.

Keywords

• Eragrostis tef
• Moleküler Markırlar
• Çeşitlilik

Thanks

Çalışma materyalini bize gönderdiği için Etiyopya Wolla Üniversitesinden Dr. Seid Hussen Muhie’ye teşekkür ederiz.

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