Isolation of Cellulase Producing Bacteria from Diyadin Hot Springs and Investigation of Exopolysaccharide Production Ability

Abstract

In this study, bacterial isolation was carried out from the hot spring water source and surrounding soil samples located approximately 5 km from the center of Diyadin district in Ağrı province. The degradation of cellulose holds significant industrial importance across a broad spectrum of fields, including the food industry, textile and paper manufacturing, pharmaceuticals, medical applications, and animal nutrition. In this context, the aim of the study was to isolate a bacterium with high cellulolytic activity and exopolysaccharide (EPS) production capacity to contribute to potential biotechnological applications. As a result of the isolation studies, a total of 22 morphologically distinct strains were obtained as pure cultures. Among these, 14 strains exhibited cellulolytic activity, and the strain showing the highest activity was identified at the species level. Molecular analyses revealed that this isolate belonged to Bacillus licheniformis. The optimum growth pH of this bacterium, which has potential as a plant probiotic, was determined to be 6.0, while maximum EPS production was observed at 40 °C with a concentration of 110 mg/L. Antimicrobial analyses conducted during the study demonstrated that the EPS synthesized by Bacillus licheniformis possesses inhibitory effects against certain pathogenic microorganisms. These findings suggest that the isolated strain could serve as a valuable microbial resource for both industrial and biotechnological applications.

Keywords

• Bacillus licheniformis
• Cellulase
• Exopolysaccharide
• Antibacterial
• Diyadin
• Hot spring

Diyadin Kaplıcalarından Selülaz Üreten Bakteri İzole Edilmesi ve Ekzopolisakkarit Üretme Yeteneğinin İncelenmesi

Abstract

Bu çalışmada, Ağrı ili Diyadin ilçesi merkezine yaklaşık 5 km mesafede bulunan bir kaplıcanın sıcak su kaynağından ve çevresindeki toprak örneklerinden bakteri izolasyonu gerçekleştirilmiştir. Selülozun degradasyonu, gıda endüstrisinden tekstil ve kâğıt sanayisine, ilaç ve tıbbi uygulamalardan hayvan beslenmesine kadar geniş bir yelpazede endüstriyel öneme sahiptir. Bu bağlamda çalışmanın amacı, yüksek selülotik aktiviteye ve aynı zamanda ekzopolisakkarit (EPS) üretim kapasitesine sahip bir bakteri izole ederek potansiyel biyoteknolojik uygulamalara katkı sağlamaktır. İzolasyon çalışmaları sonucunda morfolojik açıdan farklılık gösteren toplam 22 suş saf kültür olarak elde edilmiştir. Bu suşlardan 14’ünün selülotik aktivite sergilediği belirlenmiş, en yüksek aktiviteye sahip suşun tür düzeyinde tanımlaması yapılmıştır. Moleküler analizler sonucunda bu izolatın Bacillus licheniformis olduğu saptanmıştır. Bitkiler için probiyotik potansiyeli taşıyan bu bakterinin optimum büyüme pH’ı 6.0 olarak tespit edilirken, maksimum EPS üretiminin 40 °C’de 110 mg/L seviyesinde gerçekleştiği belirlenmiştir. Çalışma kapsamında yapılan antimikrobiyal analizler, Bacillus licheniformis tarafından sentezlenen EPS’nin bazı patojen mikroorganizmaların gelişimini inhibe edici özelliğe sahip olduğunu ortaya koymuştur. Bu bulgular, izole edilen suşun hem endüstriyel hem de biyoteknolojik uygulamalar açısından değerli bir mikrobiyal kaynak olabileceğini göstermektedir.

Keywords

• Bacillus licheniformis
• Selülaz
• Ekzopolisakkarit
• Antibakteriyel
• Diyadin
• Kaplıca

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