Probiyotik Ürünlerden ve Preparatlardan İzole Edilen Mikroorganizmaların Pseudomonas aeruginosa ve Escherichia coli Üzerindeki Antimikrobiyal Aktivitesi

Abstract

Antibiyotik direncinin neden olduğu ilaca dirençli suşlar, tedavi süresinin uzaması, ölüm oranlarının artması ve sağlık maliyetlerinin yükselmesi kritik bir küresel sorun haline geldiğinden, bilim insanları probiyotik kullanımı gibi alternatif tedavi seçenekleri aramaktadır. Bu çalışmada çeşitli ev yapımı ve ticari probiyotik ürünler ile eczane preparatlarından izole edilen standart probiyotik mikroorganizmaların (Lactiplantibacillus plantarum ATCC 14917, Lactobacillus fermentum ATCC 14931, Bifidobacterium bifidum ATCC 29521 ve Bifidobacterium longum 15707) Pseudomonas aeruginosa ve Escherichia coli'nin standart ve klinik suşları üzerindeki antimikrobiyal etkilerinin araştırılması amaçlanmıştır. Probiyotik mikroorganizmaların patojen mikroorganizmalar üzerindeki antimikrobiyal aktivitesi spot on lawn yöntemi ile belirlenmiştir. Test edilen probiyotikler arasında Lactiplantibacillus plantarum, Lactobacillus kefirii, Bifidobacterium spp, L. plantarum ATCC 14917, L. fermentum ATCC 14931, B. bifidum ATCC 29521 ve B. longum ATCC 15707 tüm P. aeruginosa ile E. coli suşlarına karşı antimikrobiyal aktivite göstermiştir. Ancak eczane preparatından izole edilen Enterococcus faecium ve B. bifidum ATCC 29521 sadece E. coli'ye karşı etkili olmuş ve P. aeruginosa üzerinde herhangi bir etki göstermemiştir. Ayrıca, bu çalışmanın sonuçları bu alanda gelecekte yapılacak çalışmalara ışık tutabilir.

Keywords

• • Pseudomonas aeruginosa.
• • Escherichia coli
• • Probiyotikler
• • Antibiyotikler

Antimicrobial Activity of Microorganisms Isolated from Probiotic Products and Preparations on Pseudomonas aeruginosa and Escherichia coli

Abstract

Since the drug-resistant strains, prolonging treatment duration, increasing mortality rates, together with raising healthcare costs caused by antibiotic resistance has become a critical global problem, the scientists have been seeking alternative treatments options such as probiotic supplementation. The present study aimed to investigate the antimicrobial effects of standard probiotic microorganisms (Lactiplantibacillus plantarum ATCC 14917, Lactobacillus fermentum ATCC 14931, Bifidobacterium bifidum ATCC 29521 and Bifidobacterium longum 15707) isolated from various probiotic homemade and commercial probiotic products and pharmacy preparations on standard and clinical strains of Pseudomonas aeruginosa and Escherichia coli. The antimicrobial activity of the probiotic microorganisms on the pathogenic microorganisms was assessed by spot on lawn method. Among the tested probiotics, Lactiplantibacillus plantarum, Lactobacillus kefirii, Bifidobacterium spp. L. plantarum ATCC 14917, L. fermentum ATCC 14931, B. bifidum ATCC 29521 and B. longum ATCC 15707 showed activity against all P. aeruginosa and E. coli strains. However, Enterococcus faecium and B. bifidum ATCC 29521 isolated from pharmacy preparation were effective only against E. coli and had no effect on P. aeruginosa. Furthermore, the results of present study could shed light on the future studies in this area.

Keywords

• • Escherichia coli
• • Pseudomonas aeruginosa
• • Probiotics
• • Antibiotics

References

1. Aoudia, N., Rieu, A., Briandet, R., Deschamps, J., Chluba, J., Joly-Guillou, M. L., & Gruss, A. (2016). Biofilm formation by Lactobacillus species: Strain-dependent immunomodulation. Clinical and Experimental Immunology, 185(3), 478–488.
2. Aziz, M., Davis, G. S., Park, D. E., Idris, A. H., Sariya, S., Wang, Y., ... & Price, L. B. (2024). Pediatric urinary tract infections caused by poultry-associated Escherichia coli. Microbiology Spectrum, 12(7).
3. Flint, H. J., Duncan, S. H., Scott, K. P., & Louis, P. (2015). Links between diet, gut microbiota composition and gut metabolism. Proceedings of the Nutrition Society, 74(1), 13–22.
4. Galyon, M. (2022). Probiotics and antibiotic-induced microbial aberrations in children. Frontiers in Microbiology, 13, 11227081.
5. Gonzalez, D., & McDonald, L. C. (2014). Clinical and economic consequences of hospital-acquired resistant and multidrug-resistant Pseudomonas aeruginosa infections: A systematic review and meta-analysis. Antimicrobial Resistance & Infection Control, 3, 32.
6. Gupta, T., Kaur, H., Kapila, S., & Kapila, R. (2021). Potential probiotic Lacticaseibacillus rhamnosus MTCC-5897 attenuates Escherichia coli induced inflammatory response in intestinal cells. Archives of Microbiology, 203(9), 5703–5713.
7. Hesari, M. R., Darsanaki, R. K., & Salehzadeh, A. (2017). Antagonistic activity of probiotic bacteria isolated from traditional dairy products against E. coli O157:H7. Journal of Medical Bacteriology, 6(3–4), 23–30.
8. Johnson J. R. (1991). Virulence factors in Escherichia coli urinary tract infection. Clinical microbiology reviews, 4(1), 80–128.

9. Kaper, J. B., Nataro, J. P., & Mobley, H. L. (2004). Pathogenic Escherichia coli. Nature Reviews. Microbiology, 2(2), 123-140.
10. Karpuz, E. (2020). Bazı bitki ekstreleri ile saf fenolik bileşiklerin probiyotik ve patojen mikroorganizmaların biyofilm gelişimi üzerine etkisi (Master dissertation).
11. Kunz Coyne, A. J., El Ghali, A., Holger, D., Rebold, N., & Rybak, M. J. (2022). Therapeutic strategies for emerging multidrug-resistant Pseudomonas aeruginosa. Infectious Diseases and Therapy, 11(2), 661–682.
12. Leimbach, A., Hacker, J., & Dobrindt, U. (2013). E. coli as an all-rounder: the thin line between commensalism and pathogenicity. Current Topics in Microbiology and Immunology, 358, 3-32.
13. Makvana, S., & Krilov, L. R. (2015). Escherichia coli infections. Pediatrics in Review, 36(4), 167–170.
14. Mokoena, M. P. (2017). Lactic acid bacteria and their bacteriocins: Classification, biosynthesis and applications against uropathogens: A mini-review. Molecules, 22(8), 1255.
15. Morrison, D., Axelrod, P., & Keusch, G. T. (1984). Lactic acid bacteria and human clinical infection. Journal of Infection, 9(3), 199–210.
16. Ozduran, E. (2023). Lactobacıllus plantarum Suşlarının İnaktive Edilmiş ve Edilmemiş Formlarının Antioksidan, Antimikrobiyal Ve Antibiyofilm Etkilerinin Karşılaştırılması. (Danışman: Doç. Dr. Özlem Bingöl Özakpınar; İkinci Danışman: Dr. Öğr. Üyesi Pervin Rayaman). Marmara Üniversitesi Sağlık Bilimleri Enstitüsü. (Master dissertation).
17. Rayaman, P., & Turgan, R. E. (2026). Effect of Various Glucose Concentrations on Lactobacillus Species. Balıkesir Sağlık Bilimleri Dergisi, 15(1), 55-61.
18. Ríos-Covián, D., Salazar, N., Gueimonde, M., de los Reyes-Gavilán, C. G., & Margolles, A. (2016). Intestinal short chain fatty acids and their link with diet and human health. Frontiers in Microbiology, 7, 185.
19. Rivière, A., Selak, M., Lenoir-Wijnkoop, I., & van Hylckama Vlieg, J. E. (2016). Bifidobacteria and butyrate-producing colon bacteria: Importance and strategies for their stimulation in the human gut. Frontiers in Microbiology, 7, 979.
20. Spellberg, B., Bartlett, J. G., & Gilbert, D. N. (2013). The future of antibiotics and resistance. The New England Journal of Medicine, 368(4), 299–302.
21. Suez, J., Zmora, N., Zilberman-Schapira, G., Mor, U., Dori-Bachash, M., Bashiardes, S., ... & Elinav, E. (2018). Post-antibiotic gut mucosal microbiome reconstitution is enhanced by autologous fecal microbiome transplantation but not by probiotics. Nature Medicine, 24(12), 1824–1834.
22. Taş, E., & Erginkaya, Z. (2008). Bazı probiyotik laktik asit bakterilerinin Escherichia coli O157:H7 üzerine inhibisyon etkisi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 19(3), 25–30.
23. Tetik, N. (2017). Probiyotik ürün ve preparatların Helicobacter pylori ve Staphylococcus aureus üzerine antimikrobiyal aktivitesinin araştırılması (Master dissertation).
24. Tunalı, Y. (2014). Farmasötik Mikrobiyoloji Uygulamaları. Dora Basım Yayın Dağıtım Ltd. Şti.
25. van Baarlen, P., Wells, J. M., & Kleerebezem, M. (2013). Regulation of intestinal homeostasis and immunity with probiotic lactobacilli. Proceedings of the National Academy of Sciences of the United States of America, 110(1), 1–8.
26. Wu, W., & Huang, J. (2024). Antibiotic influx and efflux in Pseudomonas aeruginosa: Regulation and implications for antimicrobial resistance. Environmental Microbiology Reports, 16(5), 1123–1135.