Probiyotik Ürünlerden ve Preparatlardan İzole Edilen Mikroorganizmaların Pseudomonas aeruginosa ve Escherichia coli Üzerindeki Antimikrobiyal Aktivitesi

Öz

Antibiyotik direncinin neden olduğu ilaca dirençli suşlar, tedavi süresinin uzaması, ölüm oranlarının artması ve sağlık maliyetlerinin yükselmesi kritik bir küresel sorun haline geldiğinden, bilim insanları probiyotik kullanımı gibi alternatif tedavi seçenekleri aramaktadır. Bu çalışmada çeşitli ev yapımı ve ticari probiyotik ürünler ile eczane preparatlarından izole edilen standart probiyotik mikroorganizmaların (Lactiplantibacillus plantarum ATCC 14917, Lactobacillus fermentum ATCC 14931, Bifidobacterium bifidum ATCC 29521 ve Bifidobacterium longum 15707) Pseudomonas aeruginosa ve Escherichia coli'nin standart ve klinik suşları üzerindeki antimikrobiyal etkilerinin araştırılması amaçlanmıştır. Probiyotik mikroorganizmaların patojen mikroorganizmalar üzerindeki antimikrobiyal aktivitesi spot on lawn yöntemi ile belirlenmiştir. Test edilen probiyotikler arasında Lactiplantibacillus plantarum, Lactobacillus kefirii, Bifidobacterium spp, L. plantarum ATCC 14917, L. fermentum ATCC 14931, B. bifidum ATCC 29521 ve B. longum ATCC 15707 tüm P. aeruginosa ile E. coli suşlarına karşı antimikrobiyal aktivite göstermiştir. Ancak eczane preparatından izole edilen Enterococcus faecium ve B. bifidum ATCC 29521 sadece E. coli'ye karşı etkili olmuş ve P. aeruginosa üzerinde herhangi bir etki göstermemiştir. Ayrıca, bu çalışmanın sonuçları bu alanda gelecekte yapılacak çalışmalara ışık tutabilir.

Anahtar Kelimeler

• • Pseudomonas aeruginosa.
• • Escherichia coli
• • Probiyotikler
• • Antibiyotikler

Antimicrobial Activity of Microorganisms Isolated from Probiotic Products and Preparations on Pseudomonas aeruginosa and Escherichia coli

Öz

Since the drug-resistant strains, prolonging treatment duration, increasing mortality rates, together with raising healthcare costs caused by antibiotic resistance has become a critical global problem, the scientists have been seeking alternative treatments options such as probiotic supplementation. The present study aimed to investigate the antimicrobial effects of standard probiotic microorganisms (Lactiplantibacillus plantarum ATCC 14917, Lactobacillus fermentum ATCC 14931, Bifidobacterium bifidum ATCC 29521 and Bifidobacterium longum 15707) isolated from various probiotic homemade and commercial probiotic products and pharmacy preparations on standard and clinical strains of Pseudomonas aeruginosa and Escherichia coli. The antimicrobial activity of the probiotic microorganisms on the pathogenic microorganisms was assessed by spot on lawn method. Among the tested probiotics, Lactiplantibacillus plantarum, Lactobacillus kefirii, Bifidobacterium spp. L. plantarum ATCC 14917, L. fermentum ATCC 14931, B. bifidum ATCC 29521 and B. longum ATCC 15707 showed activity against all P. aeruginosa and E. coli strains. However, Enterococcus faecium and B. bifidum ATCC 29521 isolated from pharmacy preparation were effective only against E. coli and had no effect on P. aeruginosa. Furthermore, the results of present study could shed light on the future studies in this area.

Anahtar Kelimeler

• • Escherichia coli
• • Pseudomonas aeruginosa
• • Probiotics
• • Antibiotics

Kaynakça

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