Hatay’da yetişen “Helichrysum stoechas” ın Fenolik Madde ve Antioksidan Etkilerinin incelenmesi ve Esansiyel Yağ Bileşiminin Belirlenmesi

Abstract

Bu çalışma, Hatay bölgesinden toplanan H. stoechas’ın esansiyel yağ bileşimi, antioksidan kapasitesi ve

toplam fenolik içeriğini belirlemek amacıyla yapılmıştır. H. stoechas ağırlıkça % 13,55 nem içeriğine sahipken,

esansiyel yağ içeriği ağırlıkça % 0,3 oranında olup içerdiği on uçucu bileşik GC ile belirlenmiştir. Ağırlıklı olarak

uçucu bileşenler rosifoliol % 4,89, 3-metoksi-y-asaron % 34,69, elemisin % 3,24, miristisin % 23,35, apiol %

27,38’dir. Metanolde çözünen madde miktarı % 15,3 olarak bulundu. Toplam fenolik madde konsantrasyonu

256,55 ± 3,7mg Gallik asit eşdeğeri/g olarak bulunmuştur. DPPH serbest radikal süpürme aktivitesi IC50 değeri

2,76 ± 44x10-2 mg mL-1 olarak bulundu. Metanolik ekstrenin inhibisyon değeri BHA ve BHT ile karşılaştırıldı.

Uçucu yağ, halüsinojenik özelliğe sahip elemisin ve miristisin açısından zengindir. Elde edilen sonuçlar, metanolik

ekstraktının fenolik madde bakımından zengin olduğunu ve sentetik antioksidanlar BHT ve BHA’ya yakın bir

antioksidan etkinliğe sahip olduğunu gösterdi.

Keywords

• uçucu yağ,Helichrysum stoechas,antioksidant aktivite,toplam fenolik madde

Determination of Essential Oil Composition and Investigation of Phenolic Substance and Antioxidant Effect of “Helichrysum stoechas” from Hatay

Abstract

This study was carried out to determine the essential oil composition, antioxidant capacity and total

phenolic substance of Helichrysum stoechas which was gathered from Hatay region. H. stoechas had a moisture

content of 13.55 weight%, while the essential oil content of 0.3 wt% and ten volatile compounds were determined

and identified by GC. Predominantly volatile components are rosifoliol 4.89%, 3-methoxy-γ-asarone 34.69%,

elemicin 3.24%, myristicin 23.35%, apiol 27.38%. Methanol soluble fraction was found to be 15.3%. Total phenol

concentration was found 256.55 ± 3.7 mg gallic acid equivalent/g of extract powder. DPPH free radical-scavenging

activity IC50 was found as 2.76 ± 44x10-2 mg mL-1. The inhibition value of methanolic extract was compared with

BHA and BHT. The volatile oil is rich in hallucinogenic elemicin and myristicin. Obtained results showed that the

methanolic extract was rich in phenolic content and has a close antioxidant activity to the synthetic antioxidants

BHT and BHA.

Keywords

• Helichrysum stoechas,essential oil,antioxidant activity,total phenolic content

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