Hatay’da yetişen “Helichrysum stoechas” ın Fenolik Madde ve Antioksidan Etkilerinin incelenmesi ve Esansiyel Yağ Bileşiminin Belirlenmesi

Year 2018, , 197 - 203, 30.12.2018

İsmail Akça Mahir Timur

https://doi.org/10.21597/jist.422156

Cited By: 1

Abstract

Bu çalışma, Hatay bölgesinden toplanan H. stoechas’ın esansiyel yağ bileşimi, antioksidan kapasitesi ve

toplam fenolik içeriğini belirlemek amacıyla yapılmıştır. H. stoechas ağırlıkça % 13,55 nem içeriğine sahipken,

esansiyel yağ içeriği ağırlıkça % 0,3 oranında olup içerdiği on uçucu bileşik GC ile belirlenmiştir. Ağırlıklı olarak

uçucu bileşenler rosifoliol % 4,89, 3-metoksi-y-asaron % 34,69, elemisin % 3,24, miristisin % 23,35, apiol %

27,38’dir. Metanolde çözünen madde miktarı % 15,3 olarak bulundu. Toplam fenolik madde konsantrasyonu

256,55 ± 3,7mg Gallik asit eşdeğeri/g olarak bulunmuştur. DPPH serbest radikal süpürme aktivitesi IC50 değeri

2,76 ± 44x10-2 mg mL-1 olarak bulundu. Metanolik ekstrenin inhibisyon değeri BHA ve BHT ile karşılaştırıldı.

Uçucu yağ, halüsinojenik özelliğe sahip elemisin ve miristisin açısından zengindir. Elde edilen sonuçlar, metanolik

ekstraktının fenolik madde bakımından zengin olduğunu ve sentetik antioksidanlar BHT ve BHA’ya yakın bir

antioksidan etkinliğe sahip olduğunu gösterdi.

Keywords

uçucu yağ, Helichrysum stoechas, antioksidant aktivite, toplam fenolik madde

Determination of Essential Oil Composition and Investigation of Phenolic Substance and Antioxidant Effect of “Helichrysum stoechas” from Hatay

Abstract

This study was carried out to determine the essential oil composition, antioxidant capacity and total

phenolic substance of Helichrysum stoechas which was gathered from Hatay region. H. stoechas had a moisture

content of 13.55 weight%, while the essential oil content of 0.3 wt% and ten volatile compounds were determined

and identified by GC. Predominantly volatile components are rosifoliol 4.89%, 3-methoxy-γ-asarone 34.69%,

elemicin 3.24%, myristicin 23.35%, apiol 27.38%. Methanol soluble fraction was found to be 15.3%. Total phenol

concentration was found 256.55 ± 3.7 mg gallic acid equivalent/g of extract powder. DPPH free radical-scavenging

activity IC50 was found as 2.76 ± 44x10-2 mg mL-1. The inhibition value of methanolic extract was compared with

BHA and BHT. The volatile oil is rich in hallucinogenic elemicin and myristicin. Obtained results showed that the

methanolic extract was rich in phenolic content and has a close antioxidant activity to the synthetic antioxidants

BHT and BHA.

Keywords

Helichrysum stoechas, essential oil, antioxidant activity, total phenolic content

References

• Apak R, Güçlü K, Özyürek M, Karademir SE, Erçağ E, 2006. The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas. Int. J. Food Sci. Nutr. 57:292-304.
• Angioni A, Barra A, Coroneo V, Dessis S, Cabras P, 2006. Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. J. Agric. Food. Chem. 54:4364–4370.
• Bannach U, Tiebe C, Hübert T, 2012. Multigas sensors for the quality control of spice mixtures. Food Control, 26:23-27.
• Esposito ER, Bystrek MV, Klein JS, 2014. An elective course in aromatherapy science. Am. J. Pharm. Educ. 78(4):1-9.
• Grieve A, 1984. Modern Herbal. Penguin press. ISBN 0-14-046-440-9.
• Halliwell B, Gutteridge JMC, Cross CE, 1992. Free radicals antioxidants, and human disease: Where are we now?. J. Lab. Clin. Med. 119:598–620.
• Hutchings A, Scott AH, Lewis G, Cunningham AB, 1996. Zulu medicinal plants: an inventory. Pietermaritzburg: Natal University Press.
• Kalbhen DA, 1971. Nutmeg as a narcotic. A contribution to the chemistry and pharmacology af nutmeg (Myristicafragrans). Angew. Chem. Int. Ed. 10:370-374.
• Krishna A, Tiwari R, Kumar S, 2000. Aromatherapy-an alternative health care through essential oils. JMAPS, 22:798-804.
• Ksouri R, Falleh H, Megdiche W, Trabelsi N, Mhamdi B, Chaieb K, 2009. Antioxidant and antimicrobial activities of the edi ble medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food Chem. Toxicol, 47:2083-2091.
• Latha PG, Sindhu PG, Suja SR, Geetha BS, Pushpangadan P, Rajasekharan S, 2005. Pharmacology and chemistry of Myrsitica fragrans Houtt. A review. JOSAC, 14:94-101.
• Manniche L, 1999. Sacred luxuries: fragrance, aromatherapy and cosmetics in ancient Egypt. New York: Cornell University Press.
• Masotti V, Juteau F, Bessie`re JM, Viano J, 2003. Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities. J. Agric. Food. Chem. 51:7115–7121.
• Ozgen M, Scheerens JC, Reese RN, Miller RA, 2010. Total phenolic, anthocyanin contents and antioxidant capacity of selected elderberry (Sambucus canadensis L.) accessions. Pharmacogn. Mag. 6(23):198-203.
• Plants For A Future, 1996-2012. http://www.pfaf.org/User/Plant.aspx?LatinName=Helichrysum+stoechas
• Race S, 2009. Antioxidants The truth about BHA, BHT, TBHQ and other antioxidants used as food additives. Tigmor Books UK.
• Sezik E, Yesilada E, Honda G, Takaishi Y, Takeda,Y, Tanaka T, 2001. Traditional medicine in Turkey. X. Folk medicine in Central Anatolia. J. Ethnopharmacol, 75, 95–115.
• Shulgin AT, 1966. Possible implication of Myristicin as a psychotropic substance. Nature. 210:380-384.
• Singleton VL, Rossi JR, 1965. Colorimetry of total Phenolics with Phosphomolybdic- Phosphotungstic Acid Reagents. J. Enol. Vitic. 16(3):144-158.
• Suh HJ, Chung MS, Cho YH, Kim JW, Kim DH, Han KW, Kim CJ, 2005. Estimated daily intakes of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tert-butyl hydroquinone (TBHQ) antioxidants in Korea. Food Addit. Contam. A. 22(12):1176-1188.
• Truitt EB, 1967. The pharmacology of Myristicin and nutmeg. Washington: Public health service publications; 1645:215-222.
• Wang H, Cao G, Prior RL, 1996. Total antioxidant capacity of fruits. J. Agric. Food. Chem. 44:701–705.
• Weil AT, 1965. Nutmeg as a narcotic. Econ. Bot. 19:194-217.
• Zheleva-Dimitrova D, Nedialkov P, Kitanov G, 2010. Radical scavenging and antioxidant activities of methanolic extracts from Hypericum species growing in Bulgaria. Pharmacogn. Mag. 6:74-78.
• Zheng W, Wang SY, 2001. Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food. Chem. 49:5165–5170.