Total Phenolic and Antioxidant Activity of an Aqueous Extract from Zizyphus Jujube Fruit from Çanakkale, Turkey

Abstract

In this study, we investigated the antioxidant capacity and total phenolic and flavonoid contents of aqueous of Ziziphus jujuba from Çanakkale. The antioxidant capacity of the aqueous extract from Ziziphus jujuba fruits was measured by various assays including DPPH radical scavenging, nitric oksit scavenging activity, iron chelating activity and reducing power. Total phenolic and flavonoid content of the extract were measured as pyrocatechol and catechin equivalent respectively, 15.21 ± 1.05 μg pyrocatechol and 2.05 ± 0.45 μg catechin. It is especially important to support the cultivation of this fruit, which is very rich in phenolic and flavonoid substances, and to increase its use among the people. Data suggested that Ziziphus jujuba grown in Çanakkale may be importance source as natural antioxidant when it was compared with standarts and old articles about the same methods.

Keywords

• Jujube,Zizyphus Jujuba,antioxidant activity,total phenolics and flavonoids

Türkiye Çanakkale’den Hünnap Meyvesinin (Zizyphus Jujuba.) Sulu Ekstresinin Toplam Fenolik Miktarı ve Antioksidan Aktivitesi

Abstract

Bu çalışmada; Çanakkale’de yetişen Ziziphus Jujuba adlı meyvenin sulu ekstresinin antioksidan aktivitesi ve toplam fenolik, flavonoid ve antosiyanin madde miktarları tayin edildi. Antioksidan kapasite tayini için; DPPH radikalini süpürme aktivitesi, nitrik oksit giderici etkisi, Fe2+ kelatlama aktivitesi ve indirgeyici gücü belirlendi. Ekstredeki toplam fenolik ve flavonoid madde miktarları sırasıyla, 1 mg hünnap meyvesinin sulu ekstresinde 15.21 ± 1.05 μg pirokateşole eşdeğer ve 2.05 ± 0.45 μg kateşine eşdeğer olarak bulundu. Özellikle fenolik ve flavonoid madde açısından oldukça zengin olan bu meyvenin tarımının desteklenmesi ve halk arasında kullanımının arttırılması önemlidir. Çanakkale’de yetişen Ziziphus Jujuba, standartlar ve yapılmış bazı çalışmalarla karşılaştırıldığında doğal bir antioksidan madde kaynağıdır.

Keywords

• Hünnap,Zizyphus Jujuba,antioksidan aktivite,toplam fenolik ve flavonidler

References

1. Akbolat D, Ertekin C, Menges HO, Ekinci K, Erdal I, 2008. Physical and nutritional properties of jujube (Zizyphus jujube Mill.) growing in Turkey. Asian Journal of Chemistry, 20: 757-766.
2. Birt DF, Hendrich S, Wang W, 2001. Dietary agents in cancer prevention; flavonoids and isoflavonoids. Pharmacology and Therapeutics, 90: 157-177.
3. Celik Onar H, Yusufoglu A.S, Turker G, Yanardağ R, 2012. Elastase, tyrosinase and lipoxygenase inhibition and antioxidant activity of an aqueous extract from Epilobium angustifolium L. Leaves. Journal of Medicinal Plants Research, 6(5): 716-726.
4. Chung SK, Osawa T, Kawakishi S, 1997. Hydroxyl radical scavenging effects of species and scavengers from brown mustard (Brassica nigra). Bioscience, Biotechnology and Biochemistry, 61: 118-123.
5. Dorman H.D, Peltoketo A, Hiltunen R, Tikkanen M.J, 2003. Characterization of the antioxidant properties of de-odourised aqueous extracts from selected Lamaiceae herbs. Food Chemistry, 83: 255-262.
6. Ercisli S, Orhan E, 2007. Chemical composition of white (Morus alba), red (Morus rubra) and black (Morus nigra) mulberry fruits. Food Chemistry, 103: 1380-1384.
7. Geetha T, Malhotra V, Chopra K, Kaur IP, (2005. Antimutagenic and antioxidant prooxidant activity of quercetin. Indian Journal of Experimental Biology, 43: 61-67.
8. İmamoğlu H, 2016. Total Antıoxıdant Capacıty, Phenolıc Compounds And Sugar Content Of Turkey Ziziphus jujubes. Acta Scientiarum Polonorum, Hortorum Cultus, 15(5): 93-108.

9. John, J.A, Shahidi, F, 2010. Phenolic compounds and antioxidant activity of Brazil nut (Bertholletia excelsa). Journal of Functional Foods, 2: 196–209.
10. Juntachote T, Berghofer E, 2005. Antioxidative properties and stability of ethanolic extracts of Holy basil and Galangal. Food Chemistry, 92:193-202.
11. Kalt W, Forney CF, Martin A, Prior RL, 1999. Antioxidant activity, vitamin C, phenolics, and anthocyanins after fresh storage of small fruits. Journal of Agricultural and Food Chemistry. 47: 4638-4644.
12. Kamiloglu, O, Ercisli, S, Sengül, M, Toplu, C, Serçe S, 2009. Total phenolics and antioxidant activity of jujube (Zizyphus jujube Mill.) genotypes selected from Turkey. African Journal of Biotechnology. 8: 303–307.
13. Klahorst S, 2002. Exploring antioxidants. Wd Food Ingredients, (April/May): 54-59.
14. Li JW, Ding SD, Ding XL, 2005. Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube. Process Biochemistry, 40: 3607-3613.
15. Li JW, Fan LP, Ding SD, Ding S.L, 2007. Nutritional composition of five cultivars of Chinese jujube. Food Chemistry, 103: 454-460.
16. Li J, Ding S, Ding X, 2005. Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube. Process Biochemistry, 48: 3607–3613.
17. Marcocci L, Maguire J.J, Droylefaix M.T, Packer L, 2006. The Nitric Oxide-Scavenging Properties of Ginkgo Biloba Extract EGb 761. Biochemical and Biophysical Research Communications, 201: 748-755.
18. Muchuweti, M, Genda, G, Ndhlala, A.R, Kasiyamhuru, A, 2005. Sugar, organic acid and phenolic compound of Zizyphus mauritiana Lamk. fruit. European Food Research and Technology, 221: 570–574.
19. Oyaizu M, 1986. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition, 44: 307-315.
20. Ozcelik B, Lee JH, Min DB, 2006. Effects of light, oxygen and pH on absorbance of 2,2-diphenyl-1-picrylhydrazyl. Journal of Food Science, 68: 487-490.
21. Özkan Hİ, 2017. Hünnap (Zizyphus jujuba Mill.) Meyvesinin Bazı Biyokimyasal Bileşenleri ile Antibakteriyel, Hipoglisemik ve Total Antioksidan Aktivitesinin İncelenmesi. Balıkesir Üniversitesi, Sağlik Bilimleri Enstitüsü, Yüksek Lisans Tezi (Basılmış).
22. Parejoa I, Viladomat F, Bastida J, Rosas-Romero A, Saavedra G, Murcia M.A, Jime´nez A.M, Codina C, 2003. Investigation of Bolivian plant extracts for their radical scavenging activity and antioxidant activity. Life Sciences, 73: 1667-1681.
23. Sahanaka S, Tachibana Y, Okada Y, 2005. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food Chemistry, 89: 569-575.
24. San B, Yıldırım AN, 2010. Phenolic, alpha-tocopherol, beta-carotene and fatty acid composi-tion of four promising jujube (Ziziphus jujuba Miller) selections. Journal of Food Composition and Analysis, 23: 706–710.
25. Singh S, Arya R, 2011. Zizyphus: An Extroversion Gift of Nature. Journal of Sciences, 1: 15–20.
26. Slinkard K, Singleton VL, 1977. Total phenols analysis: automation and comparison with manual methods. Aerican. Journal of Enology and Viticulture, 28: 49-55.
27. Uçkaya F, 2011.Aantalya’da yetişen Ziziphus Zizyphus’un antioksidan aktivitesi ve biyokimaysal bileşiminin incelenmesi, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi (Basılmış).
28. Xu J, Chen S, Hu Q, 2005. Antioxidant activity of brown pigment and extracts from black sesame seed (Sesamum indicum L.). Food Chemistry, 91: 79-83.
29. Yen G, Duh P, Su H, Yeh C, Wu C, 2006. Scavenging effects of lotus seed extracts on reactive nitrogen species. Food Chemistry, 94: 596-602.
30. Yıldırım A, Oktay M, Bilaloğlu V, 2001. The Antioxidant Activity of the Leaves of Cydonia vulgaris. Turkısh Journal Of Medıcal Scıences, 31: 23-27.
31. Zhang H, Jiang L, Ye S, Ye Y, Ren F, 2010. Systematic evaluation of antioxidant capacities of the ethanolic extract of different tissues of jujube (Ziziphus jujuba Mill.) from China. Food and Chemical Toxicology, 48:1461-1465.
32. Zhang H, Jiang L, Ye S, Ye Y, Ren F, 2010. Systematic evaluation of antioxidant capacities of the ethanolic extract of different tissues of jujube (Ziziphus jujube Mill.) from China. Food and Chemical Toxicology, 48: 1461–1465.