Research Article
Preparation of Nanoparticle-Amplified Surface Plasmon Resonance Sensors Based on Molecular Imprinting for Pesticide Determination
Abstract
Pesticides have been utilized in agriculture for decades. However, their widespread use has increased
concerns due to their known toxicities on long term human health risks in scientific and industrial communities.
Thus, the detection of pesticides will have a great prescription due to both improve their toxicity effects over
humans and their management. In this study, poly[ethyleneglycol dimethacrylate-N-methacryloyl-(L)-tryptophan
methyl ester poly(EGDMA-MATrp) nanoparticles for sensitive, selective, fast and realtime detection chlorpyrifos
were firstly prepared and characterized by zeta sizer. Then, a chlorpyrifos-printed SPR nanosensor was prepared and
characterized by atomic force microscopy (AFM) and contact angle measurements. The thickness measurements
and AFM observations indicated that the nanoparticle thin films were almost monolayer. Chlorpyrifos sensing
ability of imprinted and nonimprinted nanosensors were investigated from chlorpyrifos solutions within the range
of 0.015-2.9 nM. Imprinted nanoparticles showed more sensitivity to chlorpyrifos than non-imprinted ones.
Langmuir adsorption model was determined as the most suitable model for this affinity system. In order to show
the selectivity of the chlorpyrifos imprinted nanoparticles, competitive adsorption of chlorpyrifos, diazinon and
parathion at 2.9 nM concentration was investigated. As a result of, SPR nanosensors have a potential to be used as
an alternative method for pesticides analysis due to fast response, easy-to-use properties, precision, selective and
real-time measurement capability.
References
- Bereli N, Saylan Y, Uzun L, Say R, Denizli A, 2011. L-histidine imprinted supermacroporous cryogels for protein recognition. Seperation Puriffication Technology, 82: 28-35.
- Chen YC, Brazier JJ, Yan MD, Bargo PR, Prahl SA, 2004. Flourescence-based optical sensor design for molecularly imprinted polymers. Sensors and Actuators B: Chemical, 102: 107-116.
- Daş YK, Aksoy A, 2016. Pestisitler. Turkiye Klinikleri J Vet Sci Pharmacol Toxicol-Special Topics, 2(2).
- Gabrieli B, Magali K, Lucila R, Martha BA, Renato Z, Osmar DP, 2016. An effective method for pesticide residues determination in tobacco by GC-MS/MS and UHPLC-MS/MS employing acetonitrile extraction with low-temperature precipitation and d-SPE clean-up. Talanta, 161: 40-47.
- Gültekin A, Ersöz A, Denizli A, Say R, 2012. Preparation of new molecularly imprinted nanosensor for cholic acid determination. Sensors and Actuators B: Chemical, 162: 153-158.
- Jamal HM, Ahmad AS, 2016. Organochlorine pesticide residues in human milk and estimated daily intake (EDI) for the infants from eastern region of Saudi Arabia. Environmental Sciences & Ecology, 164: 643-648.
- Kim N, Park IS, Kim DK, 2007. High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor. Biosensors and Bioelectronics, 22: 1593-1599.
- Kouzayha A, Rabaa AR, Iskandarani M, Beh D, Budzinski H, Jaber F, 2012. Multiresidue method for determination of 67 pesticides in water samples using solid-phase extraction with centrifugation and gas chromatography-Mass spectrometry. American Journal of Analytical Chemistry, 3: 257-265.
- Li X, Husson SM, 2006. Adsorption of dansylated amino acids on molecularly imprinted surfaces: a surface plasmon resonance study. Biosensors and Bioelectronics, 22: 336–348.
- Luzardo OP, Almeida-González M, Ruiz-Suárez N, Zumbado M, Henríquez-Hernández LA, Meilán MJ, Camacho M, Boada LD, 2015. Validated analytical methodology for the simultaneous determination of a wide range of pesticides in human blood using GC–MS/MS and LC–ESI/MS/MS and its application in two poisoning cases. Science and Justice, 55: 307–315.
- Mauriz E, Calle A, Lechuga LM, Quintana J, Montoya A, Manclús JJ, 2006. Real-time detection of chlorpyrifos at part per trillion levels in ground, surface and drinking water samples by a portable surface plasmon resonance immunosensor. Analytica Chimica Acta, 561: 40-47.
- Sari E, Üzek R, Duman M, Denizli A, 2016. Fabrication of surface plasmon resonance nanosensor for the selective determination of erythromycin via molecular imprinted nanoparticles. Talanta, 150: 607–614.
- Saylan Y, Akgönüllü S, Çimen D, Derazshamshir A, Bereli N, Yılmaz F, Denizli A, 2017. Development of surface plasmon resonance sensors based on molecularly imprinted nanofilms for sensitive and selective detection of pesticides. Sensors and Actuators B: Chemical, 241: 446-454.
- Saylan Y, Üzek R, Uzun L, Denizli A, 2014. Surface imprinting approach for preparing specific adsorbent for IgG separation. Journal of Biomaterials Science, Polymer Edition, 25: 881-894.
- Sharp DS, Eskenazi B, Harrison R, Callas P, Smith AH, 1986. Delayed health hazard of pesticide exposure. American Jornal of Publich Health, 7: 441-471.
- Therese Marie SR, Drexel HC, 2017. Green preparation and characterization of tentacle-like silver/copper nanoparticles for catalytic degradation of toxic chlorpyrifos in water. Journal of Environmental Chemical Engineering, 5: 2524-2532.
- Tuna S, Duman O, Soylu I, Kancı Bozoğlan B, 2014. Spectroscopic investigation of the interactions of carbofuran and amitrol herbicides with human serum albümin. Journal of Luminescence, 151: 22-28.
- Üzek R, Özkara S, Güngüneş H, Uzun L, Şenel S, 2014. Magnetic nanoparticles for plasmid DNA purification through hydrophobic interaction chromatography. Separation Science and Technology, 49: 2193–2203.
- Yazgan MS, 1997. Türkiye′de pestisit kirliliği. Türkiye′de Çevre Kirlenmesi Öncelikleri Sempozyumu II, 571-577. Gebze-Kocaeli.
- Yılmaz F, Saylan Y, Akgönüllü S, Çimen D, Derazshamshir A, Bereli N, Denizli A, 2017. Surface plasmon resonance based nanosensors for detection of triazinic pesticides in agricultural foods. New Pesticides and Soil Sensors, 679-718.
- Yilmaz E, Majidi D, Özgür E, Denizli A, 2015. Whole cell imprinting based Escherichia coli sensors: A study for SPR and QCM. Sensors and Actuators B, 209: 714–721.
Pestisit Tayini için Nanopartiküllerle Duyarlılığı Arttırılmış Moleküler Baskılama Temelli Yüzey Plazmon Rezonans Sensörlerin Hazırlanması
Abstract
Pestisitler yıllardır tarım alanlarında kullanılmaktadır. Ancak bu yaygın kullanım, bilimsel ve endüstriyel
topluluklarda pestisitlerin uzun vadeli insan sağlığı riskleri üzerine bilinen toksisitelerinden dolayı endişeyi
arttırmıştır. Böylelikle pestisitlerin tayin edilmesi, hem insanlar üzerinde artan toksik etkilerinden hem de
kullanımının kontrol altına alınmasından dolayı büyük bir öneme sahiptir. Bu çalışmada, seçici, hassas, hızlı ve
gerçek zamanlı klorpirifos tayini için ilk olarak, poli[etilenglikol dimetakrilat-N-metakriloil-(l)-triptofan metil
ester poly(EGDMA-MATrp) nanopartikülleri hazırlandı ve zeta sizer ile karakterize edildi. Ardından, klorpirifos
baskılı SPR nanosensör hazırlanarak atomik kuvvet mikroskobu (AFM) ve temas açısı ölçümleri ile karakterize
edildi. Kalınlık ölçümleri ve AFM görüntüleri, nanopartiküllerden oluşan ince filmlerin hemen hemen tek tabakalı
olduğunu gösterdi. Baskılanmış ve baskılanmamış nanosensörlerin klorpirifos tayin duyarlılığı 0.015-2.9 nM
aralığında klorpirifos çözeltileri ile araştırıldı. Baskılanmış nanopartiküllerin, baskılanmamışlara göre daha fazla
duyarlılık gösterdiği belirlendi. Langmuir adsorpsiyon modeli, bu afinite sistemi için en uygun model olarak
belirlendi. Klorpirifos baskılanmış nanopartiküllerin seçiciliğini göstermek için 2.9 nM derişimindeki klorpirifos,
diazinon ve parationun yarışmalı adsorpsiyonu araştırıldı. Sonuç olarak, SPR nanosensörlerin hızlı yanıt, kullanım
kolaylığı, hassasiyet, seçicilik ve gerçek zamanlı ölçüm alınabilme özelliğinden dolayı pestisit analizleri için
alternatif bir yöntem olarak kullanılma potansiyeline sahip olduğu görüldü.
References
- Bereli N, Saylan Y, Uzun L, Say R, Denizli A, 2011. L-histidine imprinted supermacroporous cryogels for protein recognition. Seperation Puriffication Technology, 82: 28-35.
- Chen YC, Brazier JJ, Yan MD, Bargo PR, Prahl SA, 2004. Flourescence-based optical sensor design for molecularly imprinted polymers. Sensors and Actuators B: Chemical, 102: 107-116.
- Daş YK, Aksoy A, 2016. Pestisitler. Turkiye Klinikleri J Vet Sci Pharmacol Toxicol-Special Topics, 2(2).
- Gabrieli B, Magali K, Lucila R, Martha BA, Renato Z, Osmar DP, 2016. An effective method for pesticide residues determination in tobacco by GC-MS/MS and UHPLC-MS/MS employing acetonitrile extraction with low-temperature precipitation and d-SPE clean-up. Talanta, 161: 40-47.
- Gültekin A, Ersöz A, Denizli A, Say R, 2012. Preparation of new molecularly imprinted nanosensor for cholic acid determination. Sensors and Actuators B: Chemical, 162: 153-158.
- Jamal HM, Ahmad AS, 2016. Organochlorine pesticide residues in human milk and estimated daily intake (EDI) for the infants from eastern region of Saudi Arabia. Environmental Sciences & Ecology, 164: 643-648.
- Kim N, Park IS, Kim DK, 2007. High-sensitivity detection for model organophosphorus and carbamate pesticide with quartz crystal microbalance-precipitation sensor. Biosensors and Bioelectronics, 22: 1593-1599.
- Kouzayha A, Rabaa AR, Iskandarani M, Beh D, Budzinski H, Jaber F, 2012. Multiresidue method for determination of 67 pesticides in water samples using solid-phase extraction with centrifugation and gas chromatography-Mass spectrometry. American Journal of Analytical Chemistry, 3: 257-265.
- Li X, Husson SM, 2006. Adsorption of dansylated amino acids on molecularly imprinted surfaces: a surface plasmon resonance study. Biosensors and Bioelectronics, 22: 336–348.
- Luzardo OP, Almeida-González M, Ruiz-Suárez N, Zumbado M, Henríquez-Hernández LA, Meilán MJ, Camacho M, Boada LD, 2015. Validated analytical methodology for the simultaneous determination of a wide range of pesticides in human blood using GC–MS/MS and LC–ESI/MS/MS and its application in two poisoning cases. Science and Justice, 55: 307–315.
- Mauriz E, Calle A, Lechuga LM, Quintana J, Montoya A, Manclús JJ, 2006. Real-time detection of chlorpyrifos at part per trillion levels in ground, surface and drinking water samples by a portable surface plasmon resonance immunosensor. Analytica Chimica Acta, 561: 40-47.
- Sari E, Üzek R, Duman M, Denizli A, 2016. Fabrication of surface plasmon resonance nanosensor for the selective determination of erythromycin via molecular imprinted nanoparticles. Talanta, 150: 607–614.
- Saylan Y, Akgönüllü S, Çimen D, Derazshamshir A, Bereli N, Yılmaz F, Denizli A, 2017. Development of surface plasmon resonance sensors based on molecularly imprinted nanofilms for sensitive and selective detection of pesticides. Sensors and Actuators B: Chemical, 241: 446-454.
- Saylan Y, Üzek R, Uzun L, Denizli A, 2014. Surface imprinting approach for preparing specific adsorbent for IgG separation. Journal of Biomaterials Science, Polymer Edition, 25: 881-894.
- Sharp DS, Eskenazi B, Harrison R, Callas P, Smith AH, 1986. Delayed health hazard of pesticide exposure. American Jornal of Publich Health, 7: 441-471.
- Therese Marie SR, Drexel HC, 2017. Green preparation and characterization of tentacle-like silver/copper nanoparticles for catalytic degradation of toxic chlorpyrifos in water. Journal of Environmental Chemical Engineering, 5: 2524-2532.
- Tuna S, Duman O, Soylu I, Kancı Bozoğlan B, 2014. Spectroscopic investigation of the interactions of carbofuran and amitrol herbicides with human serum albümin. Journal of Luminescence, 151: 22-28.
- Üzek R, Özkara S, Güngüneş H, Uzun L, Şenel S, 2014. Magnetic nanoparticles for plasmid DNA purification through hydrophobic interaction chromatography. Separation Science and Technology, 49: 2193–2203.
- Yazgan MS, 1997. Türkiye′de pestisit kirliliği. Türkiye′de Çevre Kirlenmesi Öncelikleri Sempozyumu II, 571-577. Gebze-Kocaeli.
- Yılmaz F, Saylan Y, Akgönüllü S, Çimen D, Derazshamshir A, Bereli N, Denizli A, 2017. Surface plasmon resonance based nanosensors for detection of triazinic pesticides in agricultural foods. New Pesticides and Soil Sensors, 679-718.
- Yilmaz E, Majidi D, Özgür E, Denizli A, 2015. Whole cell imprinting based Escherichia coli sensors: A study for SPR and QCM. Sensors and Actuators B, 209: 714–721.
There are 21 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Chemical Engineering |
| Journal Section | Kimya / Chemistry |
| Authors | |
| Publication Date | September 30, 2018 |
| Submission Date | December 13, 2017 |
| Acceptance Date | February 22, 2018 |
| Published in Issue | Year 2018 |