Preparation of Nanoparticle-Amplified Surface Plasmon Resonance Sensors Based on Molecular Imprinting for Pesticide Determination

Öz

Pesticides have been utilized in agriculture for decades. However, their widespread use has increased

concerns due to their known toxicities on long term human health risks in scientific and industrial communities.

Thus, the detection of pesticides will have a great prescription due to both improve their toxicity effects over

humans and their management. In this study, poly[ethyleneglycol dimethacrylate-N-methacryloyl-(L)-tryptophan

methyl ester poly(EGDMA-MATrp) nanoparticles for sensitive, selective, fast and realtime detection chlorpyrifos

were firstly prepared and characterized by zeta sizer. Then, a chlorpyrifos-printed SPR nanosensor was prepared and

characterized by atomic force microscopy (AFM) and contact angle measurements. The thickness measurements

and AFM observations indicated that the nanoparticle thin films were almost monolayer. Chlorpyrifos sensing

ability of imprinted and nonimprinted nanosensors were investigated from chlorpyrifos solutions within the range

of 0.015-2.9 nM. Imprinted nanoparticles showed more sensitivity to chlorpyrifos than non-imprinted ones.

Langmuir adsorption model was determined as the most suitable model for this affinity system. In order to show

the selectivity of the chlorpyrifos imprinted nanoparticles, competitive adsorption of chlorpyrifos, diazinon and

parathion at 2.9 nM concentration was investigated. As a result of, SPR nanosensors have a potential to be used as

an alternative method for pesticides analysis due to fast response, easy-to-use properties, precision, selective and

real-time measurement capability.

Anahtar Kelimeler

• Molecular imprinting,nanoparticles,pesticide,surface plasmon resonance sensor

Pestisit Tayini için Nanopartiküllerle Duyarlılığı Arttırılmış Moleküler Baskılama Temelli Yüzey Plazmon Rezonans Sensörlerin Hazırlanması

Öz

Pestisitler yıllardır tarım alanlarında kullanılmaktadır. Ancak bu yaygın kullanım, bilimsel ve endüstriyel

topluluklarda pestisitlerin uzun vadeli insan sağlığı riskleri üzerine bilinen toksisitelerinden dolayı endişeyi

arttırmıştır. Böylelikle pestisitlerin tayin edilmesi, hem insanlar üzerinde artan toksik etkilerinden hem de

kullanımının kontrol altına alınmasından dolayı büyük bir öneme sahiptir. Bu çalışmada, seçici, hassas, hızlı ve

gerçek zamanlı klorpirifos tayini için ilk olarak, poli[etilenglikol dimetakrilat-N-metakriloil-(l)-triptofan metil

ester poly(EGDMA-MATrp) nanopartikülleri hazırlandı ve zeta sizer ile karakterize edildi. Ardından, klorpirifos

baskılı SPR nanosensör hazırlanarak atomik kuvvet mikroskobu (AFM) ve temas açısı ölçümleri ile karakterize

edildi. Kalınlık ölçümleri ve AFM görüntüleri, nanopartiküllerden oluşan ince filmlerin hemen hemen tek tabakalı

olduğunu gösterdi. Baskılanmış ve baskılanmamış nanosensörlerin klorpirifos tayin duyarlılığı 0.015-2.9 nM

aralığında klorpirifos çözeltileri ile araştırıldı. Baskılanmış nanopartiküllerin, baskılanmamışlara göre daha fazla

duyarlılık gösterdiği belirlendi. Langmuir adsorpsiyon modeli, bu afinite sistemi için en uygun model olarak

belirlendi. Klorpirifos baskılanmış nanopartiküllerin seçiciliğini göstermek için 2.9 nM derişimindeki klorpirifos,

diazinon ve parationun yarışmalı adsorpsiyonu araştırıldı. Sonuç olarak, SPR nanosensörlerin hızlı yanıt, kullanım

kolaylığı, hassasiyet, seçicilik ve gerçek zamanlı ölçüm alınabilme özelliğinden dolayı pestisit analizleri için

alternatif bir yöntem olarak kullanılma potansiyeline sahip olduğu görüldü.

Anahtar Kelimeler

• Moleküler baskılama,nanopartikül,pestisit,yüzey plazmon rezonans sensör

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