Synthesis of Cystein-Gold Nanoparticles as Nanocarriers of Disulfıram used in Alcohol Treament

Abstract

Disulfiram (DS) is a carbamate derivative used as an alcohol deterrent. It is a relatively non-toxic substance when administered alone, but it significantly changes metabolism with alcohol. Disulfiram acts by inhibiting aldehyde dehydrogenase. If alcohol is used after disulfiram is administered, the concentration of acetaldehyde increases, followed by systemic vasodilatation, respiratory distress, nausea, hypotension, and other symptoms (acetaldehyde syndrome). It is possible that disulfiram is acting more rapidly with nanostructures such as gold nanoparticles (Au NP). Gold nanoparticles are higher potency non-toxic biomarkers when compared to quantum dots, and in this study, cysteine ​​(Cys) is focused on synthesis with Au NP as a reducing and protective agent. Gold nanoparticles of about 5 nm in diameter were conjugated to cysteine ​​(Cys) and conjugated to Cys-Au NPs as nanostructures of disulfiram, (Synthesis of gold nanoparticles, mixing in fresh Cys solutions and mixing the mixture overnight at 37 ° C in a water bath) were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), permeable electron microscopy (TEM), FT-IR, raman and UV-Vis spectroscopy.

Keywords

• Gold nanoparticles,Disulfiram,S-Au interaction,Cysteine

Alkol Tedavisinde Kullanılan Disülfiram’ın Nanotaşıyıcısı Olarak Sistein-Altın Nanopartiküllerin Sentezi

Abstract

Disülfiram (DS), alkol caydırıcı olarak kullanılan bir karbamat türevidir. Tek başına uygulandığında nispeten toksik olmayan bir maddedir, ancak alkol ile birlikte metabolizmayı belirgin bir şekilde değiştirmektedir. Disülfiram, aldehit dehidrojenazını inhibe ederek etki etmektedir. Disülfiram uygulandıktan sonra alkol alınırsa, kanda asetaldehit konsantrasyonu artar, ardından sistemik vazodilatasyon, solunum güçlüğü, bulantı, hipotansiyon ve diğer semptomlar (asetaldehit sendromu) izlenir. Disülfiramın daha hızlı bir şekilde etki göstermesi altın nanopartiküller (Au NP) gibi nanotaşıyıcılarla mümkün olmaktadır. Altın nanopartiküller, kuantum noktalarla karşılaştırıldığında daha yüksek potansiyelli toksik olmayan biyomarkerlardır ve bu çalışma kapsamında, sisteinin (Cys) indirgeyici ve koruyucu ajan olarak Au NP ile sentezine odaklanılmıştır. Yaklaşık 5 nm çapında altın nanopartiküller, Cys ile modifiye edilen ve disülfiramın nanotaşıyıcısı olarak Cys-Au NP’ler şeklinde konjugasyonu gerçekleştirilerek, (Altın nanopartiküllerin sentezi, taze Cys solüsyonlarına karıştırılarak karışım gece boyunca 37°C'de bir su banyosu içinde karıştırılarak) özellikleri taramalı elektron mikroskobu (SEM), atomik kuvvet mıkroskobu (AFM) , geçirgen elektron mıkroskobu (TEM), FT-IR, raman ve UV-Vis spektroskopisi ile değerlendirildi.

Keywords

• Altın nanopartikül,Disülfiram,S-Au etkileşimi,Sistein

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