The Effect of Location And Species Differences on Antioxidant Activities of Endemic Haplophyllum A. Juss.

Abstract

The aim of current study was to determinate antioxidant activities, the total flavonoid and phenolic contents of aerial parts of some endemic Haplophyllum A. Juss. (H. myrtifolium, H. vulcanicum, H. pumiliforme and H. sahinii) collected from different locations of Turkey. The total phenolic content of the samples ranged from 31.8 to 49.8 mg GAEg-1 extract. H. vulcanicum and H. sahinii had higher total phenolic content than other species. The highest total flavonoid amount was detected in the samples of both locations of H. myrtifolium (62.0 and 63.0 mg KE g-1 extract) whereas the lowest was reported in the samples of both locations of H. pumiliforme (36.2 and 31.7 mg KE g-1 extract). Antioxidant activities were measured by radical scavenging activities such as the 2, 2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Location1 samples of H. myrtifolium and location2 samples of H. pumiliforme showed the strongest ABTS radical scavening activity (78.6% and 79.0%, respectively). The samples in two locations of H. vulcanicum had the lowest value for two activities. As a result, H. pumiliforme was found to have low phytochemical content but exhibited high antioxidant activity. The results of antioxidant activities and total bioactive contents about H. vulcanicum, H. sahini and H. pumiliforme is reported by us for the first time.

Keywords

• H. myrtifolium,H. vulcanicum,H.pumiliforme,H. sahinii,antioxidant activity

Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi

Abstract

Bu çalışmanın amacı Tükiye’nin farklı lokasyonlarından toplanmış bazı endemik Haplophyllum A. Juss. (H. myrtifolium, H. vulcanicum, H. pumiliforme ve H. sahinii) türlerinin toplam fenolik, toplam flavonoid ve antioksidan aktivitelerinin belirlenmesidir. Örneklerin toplam fenolik miktarı 31.8 ila 49.8 mg GAE g-1 ekstrakt arasında değişmiştir. H. vulcanicum ve H. sahinii diğer türlere göre daha yüksek toplam fenolik içeriğine sahip olmuştur. En yüksek toplam flavonoid miktarı H. myrtifolium türünün her iki lokasyonundaki örneklerinde (62.0 ve 63.0 mg KE g-1 ekstrakt), en düşük ise H. pumiliforme türünün her iki lokasyonundaki örneklerinde (36.2 ve 31.7 mg KE g-1 ekstrakt) tespit edilmiştir. Antioksidan aktivitesi olarak DPPH ve ABTS radikal kovucu aktiviteleri incelenmiştir. H. myrtifolium türünün lokasyon1 örnekleri ile H. pumiliforme türünün lokasyon2 örnekleri en yüksek ABTS radikal kovucu aktivitesi sergilemiştir (sırasıyla %78.6 ve %79.0). H. vulcanicum türünün iki lokasyonundaki örnekleri ise iki aktivite içinde en düşük değer göstermiştir. Sonuç olarak, H. pumiliforme türünün düşük fitokimyasal içerdiğine sahip olduğu, fakat yüksek oranda antioksidan aktivite sergilediği tespit edilmiştir. H. vulcanicum, H. sahini ve H. pumiliforme hakkındaki toplam biyoaktif madde içeriği ve antioksidan aktivitesi ilk kez tarafımızdan bildirilmiştir.

Keywords

• H. myrtifolium,H. vulcanicum,H.pumiliforme,antioksidan,H. sahinii

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