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Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province

Year 2020, , 2366 - 2377, 15.12.2020

Mustafa Usta , Abdullah Güller

https://doi.org/10.21597/jist.719099
Cited By: 3

Abstract

Virus-like symptoms were observed in alfalfa plants (Medicago sativa L.) grown in Van province of Turkey in 2019. The symptoms observed were dwarfing, mosaic pattern and yellowing of leaves, rosetting, and decreasing in leaf sizes. Genomic RNA of 19 symptomatic and non-symptomatic alfalfa leaves were extracted for the detection of virus RNA using virus-specific primer pair by RT-PCR. Of the 19 alfalfa leaf specimens, the six yielded the expected 700 bp DNA band in severely symptomatic alfalfa specimens. Two of them randomly selected were inserted into a plasmid vector. Obtained recombinant plasmids were sequenced in both directions. According to the results of the sequence analysis, it was revealed that the virus in infected alfalfa was the Alfalfa mosaic virus. The sequence data were recorded into the GenBank with access numbers MT210179 and MT210178 and denominated as Alakoy Y9 and Alakoy Y1 isolates, respectively. According to the phylogenetic tree created with 16 AMV sequences registered in the GeneBank for both sequences, the AMV CP gene sequence indicated the highest similarity with USA (JN256023), Brazil (FJ858265), and Puglia (Y09110) isolate and the lowest with Korea isolate (KY348844), at the nucleotide level. It was also revealed that both sequences show 98.45% nucleotide similarity with 7 nucleotide exchanges. According to the literature research results, this is the first report and molecular analysis of Alfalfa mosaic virus (AMV) in Medicago sativa L. in Van province of Turkey.

Keywords

Alfalfa RT-PCR characterisation sequencing phylogenetic analysis

References

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  • Al-Shahwan IM, Abdalla OA, Al-Saleh MA, Amer MA, 2017. Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi Journal of Biological Sciences, 24 (6): 1336- 1343.
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Van İlinde Yoncada Saptanan Yonca mozaik virüs (AMV) İzolatlarının Kılıf Protein Genomunun Moleküler Karakterizasyonu

Year 2020, , 2366 - 2377, 15.12.2020

Mustafa Usta , Abdullah Güller

https://doi.org/10.21597/jist.719099
Cited By: 3

Abstract

Van ilinde yetiştiriciliği yapılan yonca (Medicago sativa L.) bitkilerinde 2019 yılında virüslerin oluşturduğu simptomlara benzer belirtiler görülmüştür. Gözlenen belirtiler arasında cüceleşme, yapraklarda mozaik deseni ve sararma, rozetlenme ve küçük yaprak oluşumu yer almaktadır. Simptom gösteren ve göstermeyen bitkilerden toplanan 19 yonca yaprak örneğine, virüs genomunu tespit edebilmek için uygun primer çiftleri ile RT-PCR testi uygulanmıştır. Yonca yaprağı örneklerinden şiddetli belirtiye sahip altısı beklenen büyüklükte 700 bp DNA band oluşturmuştur. Bunlar arasından rastgele seçilen ikisi bir plazmid vektörüne klonlanmıştır. Elde edilen rekombinant plazmidler her iki yönde dizilenmiştir. Dizi analizi sonuçlarına göre infekteli yoncalardaki virüsün Yonca mozaik virüs olduğu açığa çıkarılmıştır. Dizi bilgileri MT210179 ve MT210178 erişim numaraları ile gen bankasına yüklenmiştir ve sırasıyla Alakoy Y9 ve Alakoy Y1 olarak isimlendirilmiştir. Her iki dizi için gen bankasına kayıtlı 16 AMV dizisi ile oluşturulan filogenetik ağaca göre, her iki izolat da nükleotit düzeyinde ABD, Brezilya ve Puglia izolatları ile en yüksek benzerlik ve Güney Kore izolatı ile en düşük benzerlik oranı göstermiştir. Ayrıca her iki dizinin birbirleri arasında, 7 nükleotit değişikliği ile %98.45 oranında nükleotit benzerliği gösterdiği ortaya çıkarılmıştır. Gerçekleştirilen litaratür tarama çalışmalarına göre, bu çalışma Türkiye'nin Van ilinde yetiştirilen yonca bitkilerindeki Yonca mozaik virüsü (AMV)’ nün ilk raporu ve moleküler analizidir.

Keywords

Dizileme filogenetik analiz RT-PCR karakterizasyon yonca

References

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  • Alhudaib KA, 2019. Serological and Molecular Detection of Alfalfa mosaic virus in the Major Potato Growing Areas of Saudi Arabia. Scientifc Journal of King Faisal University (Basic and Applied Sciences), 20 (1): 73- 82.
  • Ali H, Gholam K, 2011. Detection and Identification of Clavibacter michiganensis subsp. insidiosus inducing Alfalfa Wilt Disease Using Specific PCR and Physiological Methods in Iran. Research Journal of Chemistry and Environment, 15 (2): 486-491.
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  • Al-Shahwan IM, Abdalla OA, Al-Saleh MA, Amer MA, 2017. Detection of new viruses in alfalfa, weeds and cultivated plants growing adjacent to alfalfa fields in Saudi Arabia. Saudi Journal of Biological Sciences, 24 (6): 1336- 1343.
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There are 87 citations in total.

Details

Primary Language English
Subjects Agronomy
Journal Section Bitki Koruma / Plant Protection
Authors

Mustafa Usta 0000-0002-3940-2774

Abdullah Güller 0000-0003-3887-4208

Publication Date December 15, 2020
Submission Date April 13, 2020
Acceptance Date July 4, 2020
Published in Issue Year 2020

Cite

APA Usta, M., & Güller, A. (2020). Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Journal of the Institute of Science and Technology, 10(4), 2366-2377. https://doi.org/10.21597/jist.719099
AMA Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. December 2020;10(4):2366-2377. doi:10.21597/jist.719099
Chicago Usta, Mustafa, and Abdullah Güller. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology 10, no. 4 (December 2020): 2366-77. https://doi.org/10.21597/jist.719099.
EndNote Usta M, Güller A (December 1, 2020) Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Journal of the Institute of Science and Technology 10 4 2366–2377.
IEEE M. Usta and A. Güller, “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”, Iğdır Üniv. Fen Bil Enst. Der., vol. 10, no. 4, pp. 2366–2377, 2020, doi: 10.21597/jist.719099.
ISNAD Usta, Mustafa - Güller, Abdullah. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology 10/4 (December 2020), 2366-2377. https://doi.org/10.21597/jist.719099.
JAMA Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. 2020;10:2366–2377.
MLA Usta, Mustafa and Abdullah Güller. “Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province”. Journal of the Institute of Science and Technology, vol. 10, no. 4, 2020, pp. 2366-77, doi:10.21597/jist.719099.
Vancouver Usta M, Güller A. Molecular Characterization of the Coat Protein Genome of Alfalfa Mosaic Virus (AMV) Isolates from Alfalfa in Van Province. Iğdır Üniv. Fen Bil Enst. Der. 2020;10(4):2366-77.

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