Effect of locally isolated phosphate-solubilizing Pseudomonas aeruginosa IBP26 on the growth parameters of chickpea seedling

Year 2021, , 896 - 905, 01.06.2021

Muhammed Emin Cogender Nazlı Pınar Arslan , Mehmet Nuri Aydoğan

https://doi.org/10.21597/jist.819562

Abstract

This work was conducted to examine the effect of locally isolated phosphate-solubilizing bacteria on the growth parameters of chickpea seedling cultivated in pots containing Ca3(PO4)2 (tricalcium phosphate). Among the isolated strains, the highest phosphate-solubilizing activity in the broth medium was observed for the isolate IBP26. Similarly, in the greenhouse study, the same isolate was determined to cause maximum increases in growth parameters (shoot and root length, total biomass, phosphorus, total sugar, and protein contents) of chickpea plants in the presence of Ca3(PO4)2. The isolate IBP26 was identified as Pseudomonas aeruginosa (GenBank accession number: MW092004). Experiments demonstrated that the application of the bacterium alone in the absence of Ca3(PO4)2 or the application of Ca3(PO4)2 alone in the absence of bacterium did not cause significant change in growth parameters of the chickpea plants, and that the desired increases in the growth parameters of these plants could be achieved by the co-application of bacterium and Ca3(PO4)2.

Keywords

Pseudomonas aeruginosa IBP26, phosphate solubilization, chickpea, growth parameters

Lokal olarak izole edilen fosfat çözücü Pseudomonas aeruginosa IBP26’ nın nohut fidelerinin büyüme parametreleri üzerine etkisi

Abstract

Bu çalışma, lokal olarak izole edilmiş fosfat çözücü bakterilerinin, Ca3(PO4)2 (trikalsiyum fosfat) içeren saksılarda yetiştirilen nohut fidelerinin büyüme parametreleri üzerindeki etkisini incelemek için yapılmıştır. İzole edilen suşlar arasında, sıvı kültürde en yüksek fosfat çözündürücü aktivite IBP26 izolatı için gözlenmiştir. Benzer şekilde, sera çalışmasında da, aynı izolatın Ca3(PO4)2 varlığında nohut bitkilerinin büyüme parametrelerinde (sürgün ve kök uzunluğu, biyokütle, fosfor, toplam şeker ve protein içeriği) maksimum artışa neden olduğu belirlenmiştir. IBP26 izolatı Pseudomonas aeruginosa olarak teşhis edilmiştir (GenBank accession number: MW092004). Deneyler, Ca3(PO4)2' nin yokluğunda tek başına bakteri uygulamasının veya bakteri yokluğunda tek başına Ca3(PO4)2 uygulamasının nohut bitkilerinin büyüme parametrelerinde kayda değer değişime neden olmadığını, bu bitkilerinin büyüme parametrelerinde istenilen artışların bakteri ve Ca3(PO4)2' nin birlikte uygulanması sayesinde başarılabileceğini göstermiştir.

Keywords

Pseudomonas aeruginosa IBP26, fosfat çözünürlüğü, nohut, büyüme parametreleri

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