Promoting effects of melatonin supplements on the embryogenic callus maintainance in alfalfa (Medicago sativa L.)

Year 2021, , 927 - 932, 01.06.2021

İsmail Bezirganoglu

https://doi.org/10.21597/jist.851610

Cited By: 1

Abstract

The goal of this study was to determine melatonin impacts on the embryogenic callus formation and callus necrosis recovering of alfalfa (Medicago sativa L). Calluses, obtained from leaf explants, were cultivated on Murashige and Skoog (MS) medium supplemented with 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D or 0.0125 mg mL-1 kinetin + 1 mg mL-1 melatonin. Melatonin reduced the embryogenic callus formation in both concentrations. It was detected that the weight of fresh callus in genotypes was strongly related with hormones under in vitro conditions and the lowest fresh callus weight was detected in presence of 0.0125 mg mL-1 kinetin and 1 mg mL-1 melatonin. Based on the callus necrosis recovery, the highest callus necrosis weight (0.843 g) were recorded in embryogenic calluses cultivated in 0.0125 mg mL-1 melatonin + 1 mg mL-1 2,4-D melatonin-supplemented medium, compared with the calluses activited with MS alone (0.587 g). In conclusion, it was detected in this study that in vitro melatonin could be used in callus necrosis recovered in tissue cultures of alfalfa.

Keywords

Melatonin, alfalfa, Embryogenic callus, In vitro medium

References

• Adil M, Abbasi BH, Khan T, 2015. Interactive effects of melatonin and light on growth parameters and biochemical markers in adventitious roots of Withania somnifera L. Plant Cell Tissue Organ Cult, 123: 405–412.
• Chaudhary D P, Jat S L, Kumar A, Kumar B, 2014. Fodder quality of maize ıts preservation. Springer India, 153-160.
• Erdal S, Turk H, 2016. Cysteine-induced upregulation of nitrogen metabolism-related genes and enzyme activities enhance tolerance of maize seedlings to cadmium stress. Environ Exper Bot, 132:92–99.
• Erland LAE, Saxena PK, Murch SJ, 2017. Melatonin in plant signalling and behaviour. Funct Plant Biol, https://doi.org/10.1071/FP16384.
• Hardeland R, 2013. Melatonin and the theories of aging a critical appraisal of melatonin’s role in antiaging mechanisms. J Pineal Res, 55:325–356.
• Hernández-Ruiz J, Arnao MB, 2008. Melatonin stimulates the expansion of etiolated lupin cotyledons. Plant Growth Regul, 55:29–34.
• Kim M, Seo H, Park C, Park WJ, 2016. Examination of the auxin hypothesis of phytomelatonin action in classical auxin assay systems in maize. J Plant Physiol, 190:67–71.
• Koyama FC, Carvalho TLG, Alves E, da Silva HB, de Azevedo MF, Hemerly AS, Garcia CRS, 2013. The structurally related auxin and melatonin tryptophan-derivatives and their roles in Arabidopsis thaliana and in the human malaria parasite Plasmodium falciparum. J Eukaryot Microbiol, 60:646–651.
• Murashige T, and F A Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiology, 15: 473-97.
• Murch SJ, Campbell SSB, Saxena PK, 2001. The role of serotonin and melatonin in plant morphogenesis regulation of auxin-induced root organogenesis in in vitro-cultured explants of St. John's wort (Hypericum perforatum L.) In Vitro Cell Dev Biol Plant, 37:786–793.
• Murch SJ, Saxena PK, 2002. Mammalian neurohormones potential significance in reproductive physiology of St. John's wort (Hypericum perforatum L.)? Naturwissenschaften, 89:555–560.
• Pelagio-Flores R, Muñoz-Parra E, Ortíz-Castro R, López-Bucio J, 2012. Melatonin regulates Arabidopsis root system architecture likely acting independently of auxin signaling. J Pineal Res, 53:279–288.
• Ramakrishna A, Giridhar P, Jobin M, Paulose CS, Ravishankar GA, 2011. Indoleamines and calcium enhance somatic embryogenesis in Coffea canephora Pex Fr. Plant Cell Tissue Organ Cult, 108:267– 278.
• Ramakrishna A, Giridhar P, Sankar KU, Ravishankar GA, 2012. Endogenous profiles of indoleamines serotonin and melatonin in different tissues of Coffea canephora Pex Fr. As analyzed by HPLC and LC-MS-ESI. Acta Physiol Plant, 34:393–396.
• Reiter RJ, Tan DX, 2002. Melatonin an antioxidant in edible plants. Ann N YAcad Sci, 957:341–344.
• Sakiroglu M., JJ Doyle, EC Brummer, 2010. Inferring population structure and genetic diversity of broad range of wild diploid alfalfa (Medicago sativa L.) accessions using SSR markers. Theor Appl Genet, 121:403–415.
• Sarrou E, Therios I, Dimassi-Theriou K, 2014. Melatonin and other factors that promote rooting and sprouting of shoot cuttings in Punica granatum cv. Wonderful. Turk J Botany, 38:293–301.
• Turk H, Erdal S, Genisel M, Atici O, Demir Y, Yanmis D, 2014. The regulatory efect of melatonin on physiological, biochemical and molecular parameters in cold-stressed wheat seedlings. Plant Growth Regul, 74:139–152.
• Ullah M.A, Tungmunnithum D, Garros L, Drouet S, Hano C, Abbasi BH, 2019. Effect of Ultraviolet‐C Radiation and Melatonin Stress on Biosynthesis of Antioxidant and Antidiabetic Metabolites Produced in In Vitro Callus Cultures of Lepidium sativum L. Int. J Mol Sci, 20:1787.
• Yazıcılar Y, Böke F, Alaylı A, Nadaroğlu H, Gedikli S, Bezirganoğlu I, 2021. In vitro effects of CaO nanoparticles on Triticale callus exposed to short and long-term salt stress. Plant Cell Report, 40:29-42.