Production and Characterization of Xylanase from Streptomyces sp. which is Isolated from Soil

Year 2017, Volume: 7 Issue: 3, 115 - 123, 30.09.2017

Barış Enez Sema Agüloğlu Fincan

Abstract

In this work, Streptomyces sp., was isolated from soil, identifed and optimized. Optimum conditions
of Streptomyces sp. were determined and it is observed that maximum reproduction is achieved at 35 oC, pH 7.0
and 52 hours. It was also revealed that the best conditions for producing xylanase was 35 oC, pH 7.0 and 72 hours
and the best enzyme activity was achieved at 40 °C and pH 7.0. The effect of metals on enzyme activity was
investigated, and it was observed that enzyme activity was strongly inhibited by Zn 2+, Hg 2+ and Fe 2+. It was found
that Triton X-100, one of the detergents was used, boosts activity, while other one, SDS, inhibits strongly and it is
revealed that, enzyme activity remains stable for 1 hour at 35oC, 40 oC and 45oC and also it is preserved 100% after
1 hour at pH 6.0 and pH 7.0.

Keywords

Streptomyces sp, xylanase, isolation, characterization, production

Topraktan İzole Edilen Streptomyces sp.’den Ksilanaz Üretimi ve Karakterizasyonu

Abstract

Bu çalışmada topraktan izole edilen Streptomyces sp.’nin izolasyonu, tanımlanması ve optimizasyonu
gerçekleştirilmiştir. Streptomyces sp.’nin optimum şartları belirlenerek optimum 35 oC, pH 7.0 ve 52. saat olarak
maksimum üreme gerçekleştirdiği tespit edilmiştir. Ksilanaz üretiminin en iyi olduğu koşullar ise 35 oC, pH 7.0
ve 72. saat olarak belirlenmiş ve en iyi enzim aktivitesine 40oC ve pH 7.0’de ulaşılmıştır. Enzim aktivitesi üzerine
metallerin etkisi araştırıldığında Zn 2+, Hg 2+ ve Fe 2+ elementlerinin güçlü bir şekilde enzim aktivitesini inhibe
ettiği gözlemlenmiştir. Kullanılan deterjanlardan Triton X-100 aktiviteyi arttırırken SDS ise güçlü şekilde inhibe
ettiği tespit edilmiş ve enzim aktivitesinin; 35oC-45oC aralıklarında da 1 saat boyunca stabil kaldığı, pH 6.0, ve 7.0
de 1 saat sonunda %100 korunduğu görülmüştür

Keywords

Streptomyces sp., ksilanaz, izolasyon, karakterizasyon, üretim

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