Ratlarda Rasyona Katılan Glütenlerin Serum Lipid Profili Üzerine Etkisi

Abstract

Bu çalışmada ratların rasyonunaprotein kaynağı olarak katılan soya küspesi, mısır ve buğday glütenlerinin total kolesterol, trigliserit, düşük dansiteli lipoprotein (LDL), yüksek dansiteli lipoprotein (HDL) ve çok düşük dansiteli lipoprotein (VLDL) seviyeleri üzerine etkilerinin belirlenmesi amaçlandı. Araştırmada 20 günlük toplam 24 adet erkek rat kullanıldı ve üç grup (her grupta 8 rat) oluşturuldu. Grup1’de rasyonda protein kaynağı olarak yüksek oranda soya küspesi, Grup2’de rasyonda protein kaynağı olarak yüksek oranda mısır glüteni ve Grup3’te rasyonda protein kaynağı olarak yüksek oranda buğday glüteni kullanıldı. Çalışmanın sonunda hayvanlardan kan serumları alındı ve analizleri yapıldı. Çalışmada serum kolesterol ve HDL seviyelerinin Grup2’de önemli oranda azaldığı tespit edildi.Bununla birlikte serum trigliserit ve VLDL seviyelerinin Grup3’te önemli oranda arttığı, serum LDL seviyesinin ise tüm gruplarda azaldığı belirlendi. Sonuç olarak rasyona katılan mısır ve buğday glütenlerinin erkek ratlarda lipid profilini kısmen etkilediği söylenebilir.

Keywords

• Glüten
• Lipid Profili
• Rat
• Serum

Effect of Dietary Glutens on Lipid Profile in Rats

Abstract

This study was aimed to determine the effectiveness in diet which content of soybean meal, corn and wheat glutens of rats on serum total cholesterol, triglycerides, low density lipoproteins (LDL), high density lipoproteins (HDL) and very low density lipoproteins (VLDL). In this study, a total of 24 male rats were used which were 20 days old aged. In the study, groups were designed in 3 groups (8 animals in each group); The Group1 which used of soybean meal as a protein source in diet, the Group2 which used of corn gluten as a protein source in diet and the Group3 which used of wheat gluten as a protein source in diet as respectively. Sera were taken from the animals at the end of the experiment and analyses were performed. In this study, the levels of serum cholesterol and HDL were decreased significantly in the Group2. Furthermore, the levels of serum triglycerides and VLDL were significantly increased in the Group3, while the level of serum LDL was found similarly in all groups. As a conclusion, it is point out that the wheat and corn gluten in dietary may partially affect the lipid profile of the male rats.

Keywords

• Gluten
• Lipid Profile
• Rat
• Serum

References

1. 1.Alçay AÜ., Ahmetoğlu F., 2020. Glütenle İlişkili Rahatsızlıklar ve Glütensiz Ekmek Üretimi. Aydın Gastronomy, 4(2), 135-148.
2. 2.Anderson JW., Johnstone BM., Cook-Newell ME., 1995. Meta-analysis of the effects of soyprotein intake on serum lipids. N Engl J Med, 333(5), 276-282.
3. 3.Bonaldo A., Di Marco P., Petochi T., Marino G., Parma L., Fontanillas R., Gatta PP., 2015. Feeding turbot juveniles P setta maxima L. with increasing dietary plant protein levels affects growth performance and fish welfare. Aquac Nutr, 21(4), 401-413.
4. 4.Bong HY., Kim JY., Jeong HI., Moon MS., Kim J., Kwon O., 2010. Effects of corn gluten hydrolyzates, branched chain amino acids, and leucine on body weight reduction in obese rats induced by a high fat diet. Nutr Res Pract, 4(2), 106-113.
5. 5.Carroll KK., 1982. Hypercholesterolemia and atherosclerosis: effects of dietary protein. Fed Proc, 41(11), 2792-2796.
6. 6.Chen JH., Song J., Chen Y., Ding Q., Peng A., Mao L., 2016. The effect of vegan protein-based diets on metabolic parameters, expressions of adiponectin and its receptors in Wistar rats. Nutrients, 8(10), 643.
7. 7.Ciclitira PJ., Moodie SJ., 2003. Coeliac disease. Best Pract Res Clin Gastroenterol, 17(2), 181-195.
8. 8.Ciclitira PJ., Ellis HJ., Lundin KE., 2005. Gluten-free diet—what is toxic?. Best Pract Res Clin Gastroenterol, 19(3), 359-371.

9. 9.Fevzioglu M., Hamaker BR., Campanella OH., 2012. Gliadin and zein show similar and improved rheological behavior when mixed with high molecular weight glutenin. J CerealSci, 55(3), 265-271.
10. 10.Gao Z., Chen G., Lu W., Wu Y., Hu B., Xu L., Phillips GO., 2021. Interfacial and emulsion-stabilizing properties of zein nanoparticles: differences among zein fractions (α-, β-, and γ-zein). Food & Function, 12(3), 1361-1370.
11. 11.Goel G.,Tye-Din JA., Qiao SW., Russell AK., Mayassi T., Ciszewski C., Anderson RP., 2019. Cytokine release and gastrointestinal symptoms after gluten challenge in celiac disease. Sci Adv, 5(8),7756.
12. 12.Goesaert H., Brijs K., Veraverbeke WS., Courtin CM., Gebruers K., Delcour JA., 2005. Wheat flour constituents: how they impact bread quality, and how to impact their functionality. Trends Food Sci Technol, 16(1-3), 12-30.
13. 13.Gumus R., Gelen SU., Koseoglu S., Ozkanlar S., Ceylan ZG., Imik H., 2018. The effects of fucoxanthin dietary inclusion on the growth performance, antioxidant metabolism and meat quality of broilers. Rev Bras Cienc Avic, 20, 487-496.
14. 14.Gümüş R., Uslu S., Aydoğdu U., İmik A., Ekici M., 2021. Investigation of the effects of glutenson serum interleukin-1 beta and tumor necrosis factor-alpha levels and the immunohistochemical distribution of CD3 and CD8 receptors in the small intestine in male rats. Braz Arch Biol Technol, 64, e21210256.
15. 15.Gündüz H., Mert N., 1997. Farklı ırklardaki ithal etçi koyunlarda serum lipoprotein düzeyleri. YYU Vet Fak Derg, 8(1-2), 25-27.
16. 16.Jenkins DJ., Mirrahimi A., Srichaikul K., Berryman CE., Wang L., Carleton A., Kris-Etherton PM., 2010. Soy protein reduces serum cholesterol by both intrinsic and food displacement mechanisms. J Nutr, 140(12), 2302-2311.
17. 17.Karagül H., Altıntaş A., Fıdancı UR., Sel T., 2000. Klinik Biyokimya. pp: 161-164, Medisan Yayınevi, Ankara.
18. 18.Lee JS., 2006. Effects of soy protein and genistein on blood glucose, antioxidant enzyme activities,and lipid profile in streptozotocin-induced diabetic rats. Life Sci, 79(16), 1578-1584.
19. 19.Lu XX., Chen XH., Tang JZ., 2000. Studies on the functional property of enzymatic modified corn protein. Food Sci, 21(12), 13-15.
20. 20.Ragi ME., El Mallah C., Toufeili I., Obeid O., 2019. Concomitant lysine and phosphorus addition to a wheat gluten protein diet highly amplified growth measures of rats. Nutrition, 63, 69-74.
21. 21.SPSS., 2011. Statistical Packages for the Social Sciences. 20 ed. IBM Inc., Chicago.22.Turasan H., Kokini JL., 2017. Advances in understanding the molecular structures and functionalities of biodegradable zein-based materials using spectroscopic techniques: a review. Biomacromolecules, 18(2), 331-354.
22. 23.Türksoy S., Özkaya B., 2006. Gluten ve Çölyak hastalığı. Türkiye, 9, 24-26.
23. 24.Wieser H., 2007. Chemistry of gluten proteins. Food Microbiol, 24(2), 115-119.
24. 25.YousefiradN., 2020. Yüksek protein diyeti alan sıçanlarda antioksidan ve inflamasyon biyobelirteçleri ile obezite gelişimi arasındaki ilişkinin nesillere bağlı değişiminin incelenmesi. İstanbul Medipol Üniversitesi, Sağlık Bilimleri Enstitüsü, Türkiye.
25. 26.Zhang L., Wang L., Xie Y., Wang P., Deng S., Qin A., Jiang X., 2019. Triple‐targeting delivery of CRISPR/Cas9 To reduce the risk of cardiovascular diseases. Angew Chem Int Ed, 58(36), 12404-12408.