Research Article
BibTex RIS Cite

Production and Characterization of Polyvinyl Alcohol (PVA)/Chitosan (CH) Wound Dressings Enriched with Hypericum perforatum extract

Year 2024, Volume: 02, 1 - 7, 31.07.2024

Abstract

Flexible, durable, and biocompatible wound dressing films with antibacterial and antioxidant properties were developed using polyvinyl alcohol (PVA) and chitosan (CH) blends enriched with Hypericum perforatum extracts. The films were fabricated using the solvent casting method with varying ratios of PVA (2% and 5%) and CH (1%), incorporating Hy-pericum perforatum extracts obtained at sol-id/liquid ratios of 1/10 and 1/20. The moisture content and uptake, water vapor transmission rate (WVTR), total phenolic content, anti-oxidant capacity, and antibacterial activity were measured. Results demonstrated that the addition of plant extracts decreased the films' moisture content while higher PVA ratios increased moisture uptake. WVTR values ranged between 440 and 510 g/m²/day, indicating appropriate moisture reg-ulation for wound healing. Films containing plant extracts exhibited enhanced total phenolic content and antioxidant activity, with radical scavenging capacities up to 50%, highlighting their potential to reduce inflammation and accelerate wound recovery. Antibacterial tests against E. coli and S. aureus showed notable inhibition zones, confirming the films' effectiveness in preventing bacterial growth. The developed PVA/CH composite films demonstrate promising features such as advanced wound dressings with improved healing capabilities, combining moisture regulation, antioxidant, and antibacterial properties.

References

  • 1. Horani RZA, Kepekçi RA, İçoğlu Hİ. Bioactive PVA nanofiber mat loaded with artichoke bracts extract as a promising wound dressing biomaterial. Materials Today Communications. 2024;38:108249.
  • 2. Pasaribu ZM, Gea S, Ilyas S, Tamrin T, Radecka I. Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen. Biomolecules. 2020;10(11):1511.
  • 3. Ahmad N, Tayyeb D, Ali I, Alruwaili NK, Ahmad W, Rehman A, Khan AH, Iqbal MS. Development and Characterization of Hemicellulose-Based Films for Antibacterial Wound-Dressing Application. Polymers. 2020;12(3):548.
  • 4. Zaman HU, Islam JMM, Khan MA, Khan RA. Physico-mechanical properties of wound dressing material and its biomedical application. Journal of the Mechanical Behavior of Biomedical Materials. 2011;4(7):1369-1375.
  • 5. Coban SN, Polatoğlu I, Eroglu E. Methyl cellulose/okra mucilage composite films, functionalized with Hypericum perforatum oil and gentamicin, as a potential wound dressing. International Journal of Biological Macromolecules. 2024;254(1): 127757.
  • 6. Chopra H, Bibi S, Kumar S, Khan MS, Kumar P, Singh I. Preparation and Evaluation of Chitosan/PVA Based Hydrogel Films Loaded with Honey for Wound Healing Application. Gels. 2022;8(2):111.
  • 7. Kamoun EA, Kenawy ERS, Chen X. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. Journal of Advanced Research. 2017;8(3):217-233.
  • 8. Zhang W, Khan A, Ezati P, Priyadarshi R, Sani MA, Rathod NB, Goksen G, Rhim J. Advances in sustainable food packaging applications of chitosan/polyvinyl alcohol blend films. Food Chemistry. 2024;443:138506.
  • 9. Alemu D, Getachew E, Mondal AK. Study on the Physicochemical Properties of Chitosan and their Applications in the Biomedical Sector. International Journal of Polymer Science. 2023;5(3):5025341.
  • 10. Villani S, Padmanabhan SK, Stoppa M, Nisi R, Calcagnile M, Alifano P, Demitri C, Licciulli A. Neem-hypericum-bacterial cellulose wound care paste characterized in vitro and in Galleria mellonella in vivo model. Carbohydrate Polymer Technologies and Applications. 2024;7:100431.
  • 11. Gokce S, Tureyen A, Gokcimen A. Effects of hypericum perforatum, clinoptilolite and hydrocolloid dressing on wound healing in diabetic rats. Wound Medicine. 2018;22:14-22.
  • 12. Guleken Z, Depciuch J, Ege H, İlbay G, Kalkandelen C, Ozbeyli D, Bulut H, Sener G, Tarhan N, Kuruca SE. Spectrochemical and biochemical assay comparison study of the healing effect of the Aloe vera and Hypericum perforatum loaded nanofiber dressings on diabetic wound. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2021;254:119639.
  • 13. Hubner P, Donati N, Quines LKM, Tessaro IC, Marcilio NR. Gelatin-based films containing clinoptilolite-Ag for application as wound dressing. Materials Science and Engineering: C. 2020;107;110215.
  • 14. Chanu NR, Bhattacharya K, Marbaniang D, Pal P, Ray S, Mazumder B. Evaluation of a novel melatonin-loaded gelatin sponge as a wound dressing. Journal of Vascular Nursing. 2022;40(1):2-10.
  • 15. Darabi NH, Kalaee M, Mazinani S, Khajavi R. GO/AgNW aided sustained release of ciprofloxacin loaded in Starch/PVA nanocomposite mats for wound dressings application. International Journal of Biological Macromolecules. 2024;266(2):130977.
  • 16. Ertürk N, Biran Ay S. Effect of Ethylene Glycol and Glycerol Concentrations on Properties of Rye-Based Films. European Journal of Science and Technology.2022;34:705-710.
  • 17. Contardi M, Ayyoub AMM, Summa M, Kossyvaki D, Fadda M, Liessi N, Armirotti A, Fragouli A, Bertorelli R, Athanassiou A. Self-Adhesive and Antioxidant Poly(vinylpyrrolidone)/Alginate-Based Bilayer Films Loaded with Malva sylvestris Extracts as Potential Skin Dressings. ACS Applied Bio Materials. 2022;5(6):2880-2893.
  • 18. Güneş S, Tıhmınlıoğlu F. Hypericum perforatum incorporated chitosan films as potential bioactive wound dressing material. International Journal of Biological Macromolecules. 2017;102:933-943.
  • 19. Sanches MAR, Camelo-Silva C, Carvalho CS, Mello JR, Barroso NG, Barros ELS, Silva PP, Pertuzatti PB. Active packaging with starch, red cabbage extract and sweet whey: Characterization and application in meat. LWT. 2021;135:110275.
  • 20. Zhu P, Zhang X, Wang Y, Li C, Wang X, Tie J, Wang Y. Wang Y, et al. Electrospun polylactic acid nanofiber membranes containing Capparis spinosa L. extracts for potential wound dressing applications. Journal of Applied Polymer Science. 2021;138: e50800.
  • 21. Öztaş N, Kara E, Demir D, Yetkin D, Ceylan S, İyigündoğdu Z. Biologically active sodium pentaborate pentahydrate and Hypericum perforatum oil loaded polyvinyl alcohol: chitosan membranes. International Journal of Biological Macromolecules. 2024;269(2):132133.
  • 22. Pereira dos Santos E, Nicácio PHM, Coêlho Barbosa F, Nunes da Silva H, Andrade ALS, Lia Fook MV, de Lima Silva SM, Farias Leite I. Chitosan/Essential Oils Formulations for Potential Use as Wound Dressing: Physical and Antimicrobial Properties. Materials. 2019;12(14):2223.
Year 2024, Volume: 02, 1 - 7, 31.07.2024

Abstract

References

  • 1. Horani RZA, Kepekçi RA, İçoğlu Hİ. Bioactive PVA nanofiber mat loaded with artichoke bracts extract as a promising wound dressing biomaterial. Materials Today Communications. 2024;38:108249.
  • 2. Pasaribu ZM, Gea S, Ilyas S, Tamrin T, Radecka I. Characterization of Bacterial Cellulose-Based Wound Dressing in Different Order Impregnation of Chitosan and Collagen. Biomolecules. 2020;10(11):1511.
  • 3. Ahmad N, Tayyeb D, Ali I, Alruwaili NK, Ahmad W, Rehman A, Khan AH, Iqbal MS. Development and Characterization of Hemicellulose-Based Films for Antibacterial Wound-Dressing Application. Polymers. 2020;12(3):548.
  • 4. Zaman HU, Islam JMM, Khan MA, Khan RA. Physico-mechanical properties of wound dressing material and its biomedical application. Journal of the Mechanical Behavior of Biomedical Materials. 2011;4(7):1369-1375.
  • 5. Coban SN, Polatoğlu I, Eroglu E. Methyl cellulose/okra mucilage composite films, functionalized with Hypericum perforatum oil and gentamicin, as a potential wound dressing. International Journal of Biological Macromolecules. 2024;254(1): 127757.
  • 6. Chopra H, Bibi S, Kumar S, Khan MS, Kumar P, Singh I. Preparation and Evaluation of Chitosan/PVA Based Hydrogel Films Loaded with Honey for Wound Healing Application. Gels. 2022;8(2):111.
  • 7. Kamoun EA, Kenawy ERS, Chen X. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. Journal of Advanced Research. 2017;8(3):217-233.
  • 8. Zhang W, Khan A, Ezati P, Priyadarshi R, Sani MA, Rathod NB, Goksen G, Rhim J. Advances in sustainable food packaging applications of chitosan/polyvinyl alcohol blend films. Food Chemistry. 2024;443:138506.
  • 9. Alemu D, Getachew E, Mondal AK. Study on the Physicochemical Properties of Chitosan and their Applications in the Biomedical Sector. International Journal of Polymer Science. 2023;5(3):5025341.
  • 10. Villani S, Padmanabhan SK, Stoppa M, Nisi R, Calcagnile M, Alifano P, Demitri C, Licciulli A. Neem-hypericum-bacterial cellulose wound care paste characterized in vitro and in Galleria mellonella in vivo model. Carbohydrate Polymer Technologies and Applications. 2024;7:100431.
  • 11. Gokce S, Tureyen A, Gokcimen A. Effects of hypericum perforatum, clinoptilolite and hydrocolloid dressing on wound healing in diabetic rats. Wound Medicine. 2018;22:14-22.
  • 12. Guleken Z, Depciuch J, Ege H, İlbay G, Kalkandelen C, Ozbeyli D, Bulut H, Sener G, Tarhan N, Kuruca SE. Spectrochemical and biochemical assay comparison study of the healing effect of the Aloe vera and Hypericum perforatum loaded nanofiber dressings on diabetic wound. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2021;254:119639.
  • 13. Hubner P, Donati N, Quines LKM, Tessaro IC, Marcilio NR. Gelatin-based films containing clinoptilolite-Ag for application as wound dressing. Materials Science and Engineering: C. 2020;107;110215.
  • 14. Chanu NR, Bhattacharya K, Marbaniang D, Pal P, Ray S, Mazumder B. Evaluation of a novel melatonin-loaded gelatin sponge as a wound dressing. Journal of Vascular Nursing. 2022;40(1):2-10.
  • 15. Darabi NH, Kalaee M, Mazinani S, Khajavi R. GO/AgNW aided sustained release of ciprofloxacin loaded in Starch/PVA nanocomposite mats for wound dressings application. International Journal of Biological Macromolecules. 2024;266(2):130977.
  • 16. Ertürk N, Biran Ay S. Effect of Ethylene Glycol and Glycerol Concentrations on Properties of Rye-Based Films. European Journal of Science and Technology.2022;34:705-710.
  • 17. Contardi M, Ayyoub AMM, Summa M, Kossyvaki D, Fadda M, Liessi N, Armirotti A, Fragouli A, Bertorelli R, Athanassiou A. Self-Adhesive and Antioxidant Poly(vinylpyrrolidone)/Alginate-Based Bilayer Films Loaded with Malva sylvestris Extracts as Potential Skin Dressings. ACS Applied Bio Materials. 2022;5(6):2880-2893.
  • 18. Güneş S, Tıhmınlıoğlu F. Hypericum perforatum incorporated chitosan films as potential bioactive wound dressing material. International Journal of Biological Macromolecules. 2017;102:933-943.
  • 19. Sanches MAR, Camelo-Silva C, Carvalho CS, Mello JR, Barroso NG, Barros ELS, Silva PP, Pertuzatti PB. Active packaging with starch, red cabbage extract and sweet whey: Characterization and application in meat. LWT. 2021;135:110275.
  • 20. Zhu P, Zhang X, Wang Y, Li C, Wang X, Tie J, Wang Y. Wang Y, et al. Electrospun polylactic acid nanofiber membranes containing Capparis spinosa L. extracts for potential wound dressing applications. Journal of Applied Polymer Science. 2021;138: e50800.
  • 21. Öztaş N, Kara E, Demir D, Yetkin D, Ceylan S, İyigündoğdu Z. Biologically active sodium pentaborate pentahydrate and Hypericum perforatum oil loaded polyvinyl alcohol: chitosan membranes. International Journal of Biological Macromolecules. 2024;269(2):132133.
  • 22. Pereira dos Santos E, Nicácio PHM, Coêlho Barbosa F, Nunes da Silva H, Andrade ALS, Lia Fook MV, de Lima Silva SM, Farias Leite I. Chitosan/Essential Oils Formulations for Potential Use as Wound Dressing: Physical and Antimicrobial Properties. Materials. 2019;12(14):2223.
There are 22 citations in total.

Details

Primary Language English
Subjects Chemical Engineering (Other)
Journal Section Research Articles
Authors

Evren Arıöz

Suzan Biran Ay 0000-0002-2968-4982

Duygu Erkmen 0000-0002-9909-5606

Publication Date July 31, 2024
Submission Date June 27, 2024
Acceptance Date July 30, 2024
Published in Issue Year 2024 Volume: 02

Cite

Vancouver Arıöz E, Biran Ay S, Erkmen D. Production and Characterization of Polyvinyl Alcohol (PVA)/Chitosan (CH) Wound Dressings Enriched with Hypericum perforatum extract. JOEBS. 2024;02:1-7.



Google Scholar                            Academic Resource Index                         Creative Commons Attribution 4.0 International License