Research Article

Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products

Volume: 23 Number: 3 May 22, 2026
TR EN

Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products

Abstract

Enzyme and microbiological preparations are effectively used to increase the nutritional and biological value of meat products. An enzyme preparation of animal origin, Protepsin, containing a complex of acid proteinases, acts in the meat system similarly to enzyme cathepsins. It was used in the study together with a dry bacterial preparation LRR containing Lactobacillus casei ssp. rhamnosus, Kocuria rosea (M. rosea). The effect of these preparations on the quality of beef featuring DFD (dark, firm, dry) syndrome with a pH level of at least 6.2 and PSE (pale, soft, exudative) pork with a pH level of 5.0-5.4 was studied during salting. Processing of raw meats was carried out after preliminary grinding them in a mincer with a diameter of plate holes of 2-6 mm. The bacterial preparation LRR was added in the amount of 0.1% to raw meat, which corresponds to a concentration of at least 106 CFU per 1 g of raw material. The enzyme preparation Protepsin was added in the amount of 0.01% to the mass of raw materials (1.0 l per 100 kg of raw materials) in the form of a 1% solution. Meat without preparations treatment was used as a control. It has been established that the use of the preparations contributes to a decrease in pH values, an increase in the content of total water, water-binding capacity, elasticity, and a decrease in values of rheological parameters. The duration of salting DFD beef was 36 hours, PSE pork was 6 hours. The water-binding capacity of DFD beef samples increased by 33.9-35.1%, of control samples – by 3.2%; of PSE pork samples – by 24.6-27.8%; of control samples – by 6.8%. The penetration force of DFD beef treated with the enzyme preparation Protepsin after salting is 1.7 times less than that of untreated beef; PSE pork – 1.1 times. The elasticity values of DFD beef and PSE pork treated with Protepsin after salting are 1.4-1.2 times less than unfermented meat, respectively. The penetration force of beef DFD and pork PSE treated with the bacterial preparation LRR after salting is 1.3 times less than in control samples. The elasticity values of DFD beef and PSE pork are 1.1-1.2 times less than in control samples.

Keywords

Project Number

Research Project “Biotransformation of raw meats and forning quality parameters of ready products” No: 42.00.03.03 Р, Ukraine

Thanks

This work was supported by the Research Project “Biotransformation of raw meats and forming quality parameters of ready products” (Project No: 42.00.03.03 Р), Ukraine.

References

  1. Anonymous. (2005). TU 9219-005-42789257-2005. Enzyme Preparation Protepsin. Specifications. CJSV Endocrine Preparations Plant, Zelenograd, Moscow, Russia. (In Russian)
  2. Anonymous. (2007). Technological Instruction for The Use of The Enzyme Preparation "Protepsin" by The Company "Prime Plus Ingredients" for The Production of Meat Products. Prime Plus Ingredients, Moscow, Russia. (In Russian)
  3. Anonymous. (2010). TU U 15.5-00419880-101-2010. Bacterial Preparations for the production of Fermented Meat Products. Specifications. Dairy and Meat Technological Institute, Kyiv, Ukraine. (In Ukrainian)
  4. Antipova, L. V., Glotova, I. A. and Rogov, I. A. (2001). Methods of Research on Meat and Meat Products. Moscow: Kolos, pp. 376, Russia. (In Russian)
  5. Antipova, L. V. and Gorbunkov, M. V. (2013). Properties of the commercial enzyme preparation "Protepsin". Bulletin of the Voronezh State University of Engineering Technologies, 4 (58): 145-147. (In Russian)
  6. Antipova, L. V. and Gorbunkov, M. V. (2014). Enzyme preparation for meat processing. Meat Row, 2:72-73. (In Russian)
  7. Antipova, L.V. and Gorbunkov, M.V. (2015). Innovative application of enzyme preparations for the processing of livestock raw materials. Innovative Development of Food Technology: Materials of the International Scientific and Technical Conference, P. 55-61, Moscow, Russia. (In Russian)
  8. Antipova, L. V. and Gorbunkov, M. V. (2016). Physicochemical and biocatalytic properties of the proteolytic complex of the preparation "Protepsin". Bulletin of the Voronezh State University of Engineering Technologies, 1:89-93. (In Russian)

Details

Primary Language

English

Subjects

Food Biotechnology

Journal Section

Research Article

Publication Date

May 22, 2026

Submission Date

January 31, 2023

Acceptance Date

May 6, 2026

Published in Issue

Year 2026 Volume: 23 Number: 3

APA
Voitsekhivska, L., Franko, O., Verbytskyi, S., Shelkova, T., Okhrimenko, Y., & Patsera, N. (2026). Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products. Tekirdağ Ziraat Fakültesi Dergisi, 23(3), 714-729. https://doi.org/10.33462/jotaf.1244091
AMA
1.Voitsekhivska L, Franko O, Verbytskyi S, Shelkova T, Okhrimenko Y, Patsera N. Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products. Tekirdağ Ziraat Fakültesi Dergisi. 2026;23(3):714-729. doi:10.33462/jotaf.1244091
Chicago
Voitsekhivska, Liubov, Olena Franko, Sergii Verbytskyi, Tetiana Shelkova, Yurii Okhrimenko, and Nataliia Patsera. 2026. “Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products”. Tekirdağ Ziraat Fakültesi Dergisi 23 (3): 714-29. https://doi.org/10.33462/jotaf.1244091.
EndNote
Voitsekhivska L, Franko O, Verbytskyi S, Shelkova T, Okhrimenko Y, Patsera N (May 1, 2026) Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products. Tekirdağ Ziraat Fakültesi Dergisi 23 3 714–729.
IEEE
[1]L. Voitsekhivska, O. Franko, S. Verbytskyi, T. Shelkova, Y. Okhrimenko, and N. Patsera, “Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products”, Tekirdağ Ziraat Fakültesi Dergisi, vol. 23, no. 3, pp. 714–729, May 2026, doi: 10.33462/jotaf.1244091.
ISNAD
Voitsekhivska, Liubov - Franko, Olena - Verbytskyi, Sergii - Shelkova, Tetiana - Okhrimenko, Yurii - Patsera, Nataliia. “Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products”. Tekirdağ Ziraat Fakültesi Dergisi 23/3 (May 1, 2026): 714-729. https://doi.org/10.33462/jotaf.1244091.
JAMA
1.Voitsekhivska L, Franko O, Verbytskyi S, Shelkova T, Okhrimenko Y, Patsera N. Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products. Tekirdağ Ziraat Fakültesi Dergisi. 2026;23:714–729.
MLA
Voitsekhivska, Liubov, et al. “Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products”. Tekirdağ Ziraat Fakültesi Dergisi, vol. 23, no. 3, May 2026, pp. 714-29, doi:10.33462/jotaf.1244091.
Vancouver
1.Liubov Voitsekhivska, Olena Franko, Sergii Verbytskyi, Tetiana Shelkova, Yurii Okhrimenko, Nataliia Patsera. Biotechnological Methods of Brining Meats Effecting Quality Characteristics of Products. Tekirdağ Ziraat Fakültesi Dergisi. 2026 May 1;23(3):714-29. doi:10.33462/jotaf.1244091