CIDR Uygulanan Kıvırcık ve Karacabey Merinosu Koyunlarda T3, T4 ve Kortizol Düzeylerinin Belirlenmesi

Abstract

Bu çalışma, östrus senkronizasyonunu sağlamak amacıyla kullanılan CIDR uygulamasının, Kıvırcık ve Karacabey Merinosu koyunlarında uygulama esnasında bir stres kaynağı olup olmadığının belirlenmesi amacıyla yürütülmüştür. Bu amaçla koyunlara CIDR aparatlarının takıldığı 1. gün, 7. gün ve çıkarıldığı 14. gün alınan kan örneklerinde T3 (triiodothyronine), T4 (thyroxine) ve kortizol hormonu seviyeleri ölçülmüştür. Bu çalışmada 40 baş Kıvırcık ve 40 baş Karacabey Merinosu olmak üzere toplam 80 baş koyun kullanılmıştır. Koyunların boyun toplardamarından her üç dönemde 10 ml kan EDTA’lı tüplere alınarak ticari RIA kitleri ile T3, T4 ve kortizol konsantrasyonları belirlenmiştir. Her üç dönemde alınan kan örneklerinde T3, T4 ve kortizol hormonu ortalama değerleri Kıvırcık koyunlarında sırasıyla 1.76 (ng/mL), 7.92 (µg/dL), 5.30 (µg/dL); Karacabey Merinosunda ise sırasıyla 1.69 (ng/mL), 7.00 (µg/dL) ve 4.19 (µg/dL) olarak bulunmuştur. T4 ve kortizol bakımından ırklar arasındaki farklılık önemli (P<0.01) bulunurken,  dönemler arasındaki farklılık sadece T3 hormonu için önemli bulunmuştur (P<0.01).

Keywords

• Kıvırcık,Karacabey Merinosu,stres,CIDR,T3,T4

Determination of T3, T4 and Cortisol Levels in CIDR applied Kıvırcık and Karacabey Ewes

Abstract

This study has been conducted in order to determine whether the CIDR application on Kıvırcık and Karacabey Merino ewes used for supplying estrous synchronization a stress factor or not. For this purpose; T3 (triiodothyronine), T4 (thyroxine) and cortisol hormone levels of sheep in their blood samples were measured on day 1 when CIDR apparatus were mounted, on day 7 and on day 14 when they were removed. In this study, 80 head of ewes in total were used which consisted of 40 head Kıvırcık and 40 head Karacabey Merino ewes. 10 ml of blood from jugular veins of sheep were taken at the specified periods into EDTA tubes, and their T3, T4 and cortisol concentrations were identified by commercial RIA kits. In Kıvırcık race, these values were identified as 1.76 (ng/mL), 7.92 (µg/dL), 5.30 (µg/dL) respectively while in Karacabey Merino race as 1.69 (ng/mL), 7.00 (µg/dL) and 4.19 (µg/dL) respectively. In terms of T4 and cortisol, the difference between races were found significant (P<0.01) while difference between the periods were found significant only for T3 hormone (P<0.01).

Keywords

• Kivircik sheep,Karacabey Merino,stress,CIDR,T3,T4

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