An Optimization Study for The Production of Origanum onites Tincture by Response Surface Methodology: Effect of Solid/Liquid Ratio, Ethanol Concentration and Storage Period

Abstract

In this study, Origanum onites was used for the production of tincture, a hydroalcholic extract, and manufacturing conditions

were optimized to determine the processing factor variable levels to increase the final quality of samples. For this purpose,

response surface methodology study was applied and Box Behnken design was used for the optimization. Three factors were

selected namely liquid/solid ratio, ethanol concentration and storage period for the preparation of samples. Total phenolic

content, antiradical activity and efficient of concentration (EC50) values were determined to evaluate the bioactivity of tincture

samples. It was observed that the total phenolic content of the tincture samples was affected by ethanol concentration and

liquid/solid ratio. Total phenolic contents of tincture samples were in the range of 964.1-7020.8 mg GAE/ L samples while the

efficient concentration (EC50) ranged from 44.88 to 89.56. Increase of solid level increased the bioactivity values of samples,

but this increment decreased the tincture yield significantly. Optimization results showed that the highest total phenolic content

would be at 50% ethanol concentration, 6 ml ethanol level which means the liquid/solid ratio is 1:5 and 15 days storage for the

tincture samples.

Keywords

• Origanum onites,tincture,bioactivity,optimization,response surface methodology

Origanum onites Tentürü Üretim Şartlarının Tepki Yüzey Yöntemi ile Optimizasyonu: Katı/Sıvı Oranı, Etanol Konsantrasyonu ve Depolama Süresinin Etkisi

Abstract

Bu çalışmada, bir hidroalkolik ekstrakt tipi olan tentür üretimi amacıyla Origanum onites bitkisi kullanılmış ve son ürün

kalitesini artırmaya yönelik olarak üretim şartlarını optimize etmek üzere proses faktör seviyelerinin belirlenmesi amaçlanmıştır.

Bu amaçla, optimizasyon işlemi için tepki yüzey yöntemi tabanlı Box-Behnken deneme tasarımından faydalanılmıştır.

Örneklerin hazırlanması amacıyla sıvı/katı oranı, etanol konsantrasyonu ve depolama süresi faktör olarak seçilmiştir. Tentür

örneklerinin biyoaktif özelliklerinin belirlenmesi amacıyla toplam fenolik madde miktarı, antiradikal kapasite ve EC50 değeri

hesaplanmıştır.Sıvı/katı oranı ve etanol konsantrasyonunun örneklerin toplam fenolik madde miktarı üzerinden etkili olduğu

görülmüştür. Örneklerin toplam fenolik madde değerleri 964.1-7020.8 mg GAE/ L, EC50 değerleri ise 44.88-89.56 aralığında

değişim göstermiştir. Örnek konsantrasyonunun artışı toplam fenolik madde miktarını artırırken, bu artış tentür veriminde

düşüşe yol açmıştır. Optimizasyon sonuçları en yüksek toplam fenolik madde miktarının, %50 etanol konsantrasyonunda, 1:5

katı/sıvı oranında, 15 günlük depolama süresi sonunda elde edilebileceğini göstermiştir.

Keywords

• Origanum onites,tentür,biyoaktivite,optimizasyon,tepki yüzey yöntemi

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