Bazı Bitki Ekstraktlarının Pseudomonas syringae pv. tomato’nun Neden olduğu Domates Bakteriyel Benek Hastalığına Antibakteriyel Etkisi

Year 2019, , 231 - 243, 30.05.2019

Feray Karabüyük , Yeşim Aysan

https://doi.org/10.33462/jotaf.529499

Cited By: 4

Abstract

Pseudomonas syringae pv. tomato (Pst)’nun neden olduğu Bakteriyel Benek Hastalığı domatesin en önemli tohum kaynaklı
bakteriyel hastalığıdır. Bu çalışmada, yirmi dokuz bitki ekstraktının antibakteriyel etkileri in vitro ve in vivo’da araştırılmıştır.
In vitro’da, Allium sativum 3 mm, Coriandrum sativum 13 mm, Eucalyptus camaldulensis 13.6 mm Pst’ye antibakteriyel etki
göstermiştir. Pst’ye karşı Minimum Engelleme Konstantrasyonu değerleri (MIC) Allium sativum için 4.25 µl/ml, Eucalyptus
camaldulensis için 3.75 µl/ml, Origanum onites için ise >128 µl/ml olarak belirlenmiştir. Allium sativum, Coriandrum sativum,
Eucalyptus camaldulensis, Origanum onites, Origanum vulgare subs. hirtum ve Zingiber officinale bitki ekstraktları tohuma
uygulanmış ve Pst’nin neden olduğu hastalık %8.8-100 oranında engellenmiştir. Çalışmada, A. sativum ve O. onites en
başarılı tohum uygulaması olarak saptanmıştır. Bitki ekstraktları domates tohumlarının çimlenmesine herhangi bir olumsuz
etki yapmamıştır. Saksı çalışmalarında, Allium sativum, Eucalyptus camaldulensis, Origanum onites, Zingiber officinale
ekstraktları yeşil aksama püskürtüldüğünde Pst’nin neden olduğu hastalık %96-99, ekstraktlar köke uygulandığında %80-100
oranında baskılanmıştır. Köke Allium sativum ekstraktı uygulamak domates fidelerinde toksik olmuştur. Sonuç olarak, bitki
ekstraktları domates bakteriyel hastalıklarının mücadelesinde yeni pestisitlerin geliştirilmesi açısından ümit vaat etmektedir. 

Keywords

Domates, Bitki Ekstraktı, Antibakteriyel etki, Tohum uygulamaları, yeşil aksam uygulamaları

Antibacterial Effects of Some Plant Extracts against Tomato Bacterial Speck Disease Caused by Pseudomonas syringae pv. tomato

Abstract

Bacterial speck disease caused by Pseudomonas syringae pv. tomato (Pst) is the most important seed-borne bacterial diseases of tomato. In this study, the antibacterial activities of aqueous twenty-nine plant extracts were investigated in vitro and in vivo conditions. In in vitro experiments, extracts of Allium sativum, Coriandrum sativum, Eucalyptus camaldulensis inhibited Pst growth with the inhibition zones 3 mm, 13 mm and 13.6 mm, respectively. Minimum Inhibition Concentrations (MIC) values against Pst determination were Eucalyptus camaldulensis 3.75 μl/ml, Allium sativum 4.25 μl/ml, and Origanum onites >128 μl/ml. The effects of Allium sativum, Coriandrum sativum, Eucalyptus camaldulensis, Origanum onites, Origanum vulgare subs. hirtum and Zingiber officinale extracts on disease incidence and severity caused by Pst recorded 8.8-100% ratios as seed treatments. The most successful seed treatments were Allium sativum and Origanum onites extracts. Additionally, no negative effect on seed germination and emergence were recorded for the tested plant extracts as seed treatments. In pot experiments, foliar spraying of Allium sativum, Eucalyptus camaldulensis, Origanum onites, Zingiber officinale extracts were reduced the disease incidence from 96% to 99% ratios, whereas the disease incidence was inhibited as 80-100% ratios by root dipping. Root dipping applications of Allium sativum extract were toxic on tomato plants in both experiments for Pst. As a result, plant extracts are promising for the development of new pesticides in the control of bacterial diseases of tomatoes.

Keywords

Tomato, plant extracts, antibacterial effect, seed treatments, foliage applications

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