Identıfıcatıon of Virus Causes of Mosaic Diseases in Zucchini Plants in the Bali Island of Indonesia

Year 2021, , 411 - 418, 07.09.2021

Ni Putu Pandawani I Ketut Wıdnyana

https://doi.org/10.33462/jotaf.707645

Cited By: 2

Abstract

Mosaic disease has become epidemic in the zucchini (Cucurbita pepo L.) plantations on the island of Bali, but the cause of the disease is not yet known with certainty, although there are allegations of common causes including the Cucumber mosaic virus (CMV), Papaya ringspot virus (PRSV), Squash mosaic virus (SqMV), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). This study aims to ascertain the cause of the mosaic disease which was carried out by observation of the symptoms, proving Koch’s postulates and using Indirect-ELISA serological tests. Indirect-ELISA method was carried out following the manufacturer’s instructions DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH) Germany using several antisera namely, Zucchini yellow mosaic virus (ZYMV, DSMZ, Germany), Cucumber mosaic virus (CMV, Agdia Inc., USA) and Papaya ringspot virus (PRSV, Agdia Inc., USA). Results showed that the occurrence of mosaic diseases in zucchini plantations with very diverse symptoms as malformation and distortion of the leaves and the fruits, blistering, vein clearing, vein banding and shoestring symptoms with disease ranging from 87.17% to 92.72%. Following of Koch’s postulatesmechanical inoculations were carried out using mosaic diseased leaf extract obtained a success of transmission rate of 100%. Where, healthy zucchini plants that were inoculated with mosaic symptomatic plants exhibit the same symptoms as mosaic symptoms found in the field. The main findings of this study are serological tests with Indirect-ELISA showed that 75% of zucchini plants were infected by ZYMV and as many as 8.33% were positive to CMV. There are no plants infected with PRSV. It can be concluded that the causes of zucchini mosaics on the island of Bali are ZYMV and CMV.

Keywords

Identification, Mosaic, Zucchini, ELİSA, ZYMV

Supporting Institution

Mahasaraswati Denpasar University

Project Number

-

Thanks

The Dean of Agrotechnology Department Agriculture Faculty, Mahasaraswati Denpasar University

References

• Abd El-Aziz, M.H. (2020). The Importance of Potato virus Y Potyvirus. J Plant Sci Phytopathol. 2020; 4: 009-015.
• Babadoost, M. 1999. Mosaic diseases of cucurbits. Erişim:[http://web. aces. uiuc. edu/vista/pdf_pubs/926. pdf]. Erişim Tarihi, 3, 2014. http://web.aces.uiuc.edu/vista/pdf_pubs/926.pdf
• Barbosa, G.D.S., Lima, J.A.D.A., Queiróz, M.A.D., Dias, R.D.C.A., & Lima, C.S. (2016). Identification and effects of mixed Infection of Potyvirus isolates with Cucumber mosaic virus in cucurbits. Rev. Caatinga, Mossoró, 29(4):1028-1035.
• Coutts, B. (2006). Virus diseases of cucurbit crops. Virus diseases of cucurbit crops., (166).
• Coutts, B. A., Kehoe, M. A., Jones, R. A. C. (2011a). Minimising losses caused by Zucchini yellow mosaic virus in vegetable cucurbit crops in tropical, sub-tropical and Mediterranean environments through cultural methods and host resistance. Virus research, 159(2) : 141-160. https://doi.org/10.1016/j.virusres.2011.04.015
• Coutts, B. A., Kehoe, M. A., Webster, C. G., Wylie, S. J., Jones, R. A. C. (2011b). Zucchini yellow mosaic virus: biological properties, detection procedures and comparison of coat protein gene sequences. Archives of virology, 156(12):2119-2131. https://doi.org/10.1007/s00705-011-1102-0
• Davis , R.F. & Mizuki, M.K . (1986) Seed transmission of zucchini yellow mosaic virus in squash. Phytopathology 76:1073
• Desbiez,C. &, Lecoq, H. (1997). Zucchini yellow mosaic virus. 1997; Plant Pathol.46: 809-829.
• Dwiwiyati, N. S. (2014). Begomovirus Characterization Causes of Curly Leaf Disease in Cucumbers (Cucumis sativus L.). (Thesis) Bogor: Bogor Agricultural University.
• Fath-Allah M., Mostafa Ahmed A., & Abou-Ela (2011). Sensitive detection of watermelon mosaic and zucchini yellow mosaic viruses from infected squash plants using serological methods and polymerase chain reaction. Egy J Experimental Biol (Bot.). 2011; 7(2): 179 – 185.
• Fletcher, J.D., Wallace, A.R., & Rogers, B.T. (2000). Potyviruses in New Zealand buttercup squash (Cucurbita maxima Duch.): yield and quality eff ects of ZYMV and WMV2 virus infections. New Zealand J Crop Horticult Sci. 2000; 28: 17-26.
• Greber, R.S., McLean, G.D., & Grice, M.S. (1987) Zucchini yellow mosaic virus in three states of Australia. Australas Plant Pathol 16:19–21
• Greber, R.S., Persley, D.M., & Herrington, M.E. (1988) Some characteristics of Australian isolates of zucchini yellow mosaic virus. Aust J Agric Res 39:1085–1094
• Hosseini, S., Mosayebi, G., Kouhi, H. D. M., Okhovat, S. (2007). Characterization of the Zucchini yellow mosaic virus from squash in Tehran province. http://hehp.modares.ac.ir/article-23-8808-en.pdf
• Jossey, S., Babadoost, M. (2008). Occurrence and distribution of pumpkin and squash viruses in Illinois. Plant disease, 92(1): 61-68. https://doi.org/10.1094/PDIS-92-1-0061
• Kece, SM.A., & Kamberoglu, M.A. 2016. Biological, serological and molecular detection of watermelon mosaic virus (WMN-2) in watermelon growing fields in eastern mediterranean region. Journal of Tekırdag Agricultural Faculty, 2016:13 (3)-156-164.
• Lecoq, H. & Pitrat, M. (1983) Field experiments on the integrated control of aphid-borne viruses in muskmel-ons. In: Plumb, R.T. and Thresh, J.M. (eds)Plant Virus Epidemiology. Blackwell, Oxford, pp. 169–176.
• Lestari, S. M., Nurhayati, E. (2014). Efficiency of Squash Mosaic Virus Transmission in Cucurbitaceae. Jurnal Fitopatologi Indonesia, 10 (3) : 81. https://doi.org/10.14692/jfi.10.3.81
• Lin, S. S., Hou, R. F., Yeh, S. D. (2000). Heteroduplex mobility and sequence analyses for assessment of variability of Zucchini yellow mosaic virus. Phytopathology, 90 (3) : 228-235. https://doi.org/10.1094/PHYTO.2000.90.3.228
• Lisa, V., Boccardo G., D’Agostino G., Dellavalle G., & D’Aquilio M. (1981). Characterization of a potyvirus that causes zucchini yellow mosaic. Phytopathology. 1981; 71: 667-672.
• Önder,S., Korkmaz, S. 2008. Determination of virus and viroid diseases on satsuma owari mandarins by biological and serological methods in edremit gulf region. Journal of Tekırdag Agricultural Faculty, 2008:5 (2)-205-214.
• Prieto, H., Bruna, A., Henrichsen P., & Munoz, C.(2001). Isolation and molecular characterization of a Chilean isolate of Zucchini yellow mosaic virus. Plant Disease. 2001; 85: 644-648. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/30823033
• Matthews, R.E.F. (1992). Fundamentals of plant virology. Academic Press.
• Nurjannah, T. (2014). Host Range of Zucchini yellow mosaic Isolate Kaboca Green virus (Cucurbita pepo L.). (thesis) Dep. Plant Protection Faculty of Agriculture IPB.
• Prieto, H., Bruna, A., Hinrihsen P., Munoz, C.(2001). Isolation and molecular characterization of a Chilean isolate of Zucchini yellow mosaic virus. Plant Disease. 2001; 85: 644-648. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/30823033
• Provvidenti, R. (1996). Diseases caused by viruses. Compendium of cucurbit diseases, 37-45.
• Romay, G., Lecoq, H., & Desbiez, C. (2014). Cucurbit crops and their viral diseases in Latin America and the Caribbean islands: A Review. J. Plant Pathol. 96(2):227-242.
• Schrijnwerkers, CCFM., Huijberts, N., & Bos, L. (1991). Zucchini yellow mosaic virus: two outbreaks in the Netherlands and seed transmissability. NethJ Pl Path. 1991; 97: 187-191.
• Simmons, H. E., Holmes, E. C., Gildow, F. E., Bothe-Goralczyk, M. A., & Stephenson, A. G. (2011). Experimental verification of seed transmission of Zucchini yellow mosaic virus. Plant Disease, 95(6) : 751-754. https://doi.org/10.1094/PDIS-11-10-0843
• Sydanmetsa, M., & Mbanzibwa, D.R. (2016). Occurrence of Cucumber mosaic virus, Zucchini yellow mosaic virus and Watermelon mosaic virus in cultivated and wild cucurbits in the coastal areas of Tanzania. Afr. J. Agric. 11(40):4062-4069.
• Tobias, I., & Palkovics, L. (2003). Characterization of Hungarian isolates of zucchini yellow mosaic virus (ZYMV, potyvirus) transmitted by seeds of Cucurbita pepo var Styriaca. Pest Management Science: formerly Pesticide Science, 59 (4) : 493-497. https://doi.org/10.1002/ps.645
• Walkey, D. G. (2012). Applied plant virology. Springer Science & Business Media.
• Zitter, T. A., Hopkins, D. L., & Thomas, C. E. (1996). Compendium of cucurbit diseases (No. 635.6 632). American Phytopathological Society.
• Zitter, T.A. & Murphy, J.F. (2009). Cucumber mosaic. The Plant Health Instructor. DOI: 10.1094/PHI-I-2009-0518-01.