Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması

Year 2018, Volume: 15 Issue: 3, 36 - 43, 15.09.2018

Sümer Horuz Yeşim Aysan

Abstract

Watermelon seedling blight and fruit blotch caused by Acidovorax
citrulli (Ac) is a
seed-borne disease. The bacterium survives in/on seeds for years. The
aim of this study was to determinate the
most favorable, reliable and fast method for detection Ac from cucurbit
seeds. Cucurbit seeds were digested using a blender and shaked for 24 h
in a nutrienth broth. The methods
seedling gow out, pathogen isolation from seed dilutions onto semi selective
media P-278 and mEBB and general medium King B, DAS-ELISA and BIO-PCR were used for detection. Water-soaked
lesions observed on cotyledons. Since DAS-ELISA and PCR tests with seed
dilutions onto King B has failed to detect Ac
from seedlots, Ac spesific PCR with dilutions from semi selective media has
successfully detect the pathogen. As a result of this study, four (one cucumber,
one melon and two watermelon seedlots) out of twenty two tested cucurbit seeds
were contaminated with Ac. This study
recommended that this BIO-PCR is safe, reliable and fast for detection Ac from cucurbit seeds without any
seedling growth.

Keywords

BIO-PCR, ELISA, karpuz, tohum

References

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