Yıl 2018, Cilt 15 , Sayı 3, Sayfalar 36 - 43 2018-09-15

Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması

Sümer Horuz [1] , Yeşim Aysan [2]


Watermelon seedling blight and fruit blotch caused by Acidovorax citrulli (Ac) is a seed-borne disease. The bacterium survives in/on seeds for years. The aim of this study was to determinate the most favorable, reliable and fast method for detection Ac from cucurbit seeds. Cucurbit seeds were digested using a blender and shaked for 24 h in a nutrienth broth. The methods seedling gow out, pathogen isolation from seed dilutions onto semi selective media P-278 and mEBB and general medium King B, DAS-ELISA and BIO-PCR were used for detection. Water-soaked lesions observed on cotyledons. Since DAS-ELISA and PCR tests with seed dilutions onto King B has failed to detect Ac from seedlots, Ac spesific PCR with dilutions from semi selective media has successfully detect the pathogen. As a result of this study, four (one cucumber, one melon and two watermelon seedlots) out of twenty two tested cucurbit seeds were contaminated with Ac. This study recommended that this BIO-PCR is safe, reliable and fast for detection Ac from cucurbit seeds without any seedling growth.


BIO-PCR, ELISA, karpuz, tohum
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Birincil Dil tr
Konular Fen
Bölüm Makaleler
Yazarlar

Yazar: Sümer Horuz (Sorumlu Yazar)
Ülke: Turkey


Yazar: Yeşim Aysan

Tarihler

Başvuru Tarihi : 16 Şubat 2018
Kabul Tarihi : 12 Mart 2018
Yayımlanma Tarihi : 15 Eylül 2018

Bibtex @araştırma makalesi { jotaf395994, journal = {Tekirdağ Ziraat Fakültesi Dergisi}, issn = {1302-7050}, eissn = {2146-5894}, address = {}, publisher = {Namık Kemal Üniversitesi}, year = {2018}, volume = {15}, pages = {36 - 43}, doi = {}, title = {Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması}, key = {cite}, author = {Horuz, Sümer and Aysan, Yeşim} }
APA Horuz, S , Aysan, Y . (2018). Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması. Tekirdağ Ziraat Fakültesi Dergisi , 15 (3) , 36-43 . Retrieved from https://dergipark.org.tr/tr/pub/jotaf/issue/39525/395994
MLA Horuz, S , Aysan, Y . "Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması". Tekirdağ Ziraat Fakültesi Dergisi 15 (2018 ): 36-43 <https://dergipark.org.tr/tr/pub/jotaf/issue/39525/395994>
Chicago Horuz, S , Aysan, Y . "Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması". Tekirdağ Ziraat Fakültesi Dergisi 15 (2018 ): 36-43
RIS TY - JOUR T1 - Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması AU - Sümer Horuz , Yeşim Aysan Y1 - 2018 PY - 2018 N1 - DO - T2 - Tekirdağ Ziraat Fakültesi Dergisi JF - Journal JO - JOR SP - 36 EP - 43 VL - 15 IS - 3 SN - 1302-7050-2146-5894 M3 - UR - Y2 - 2018 ER -
EndNote %0 Tekirdağ Ziraat Fakültesi Dergisi Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması %A Sümer Horuz , Yeşim Aysan %T Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması %D 2018 %J Tekirdağ Ziraat Fakültesi Dergisi %P 1302-7050-2146-5894 %V 15 %N 3 %R %U
ISNAD Horuz, Sümer , Aysan, Yeşim . "Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması". Tekirdağ Ziraat Fakültesi Dergisi 15 / 3 (Eylül 2018): 36-43 .
AMA Horuz S , Aysan Y . Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması. Tekirdağ Ziraat Fakültesi Dergisi. 2018; 15(3): 36-43.
Vancouver Horuz S , Aysan Y . Kabakgil Tohumlarında Acidovorax citrulli’nin Aranmasında Kullanılabilecek Uygun Yöntemin Saptanması. Tekirdağ Ziraat Fakültesi Dergisi. 2018; 15(3): 43-36.