Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents

Marwa Jassim Al-Muhamadawi; Rehab Abdul Mahdi Al-Hassani

doi:10.18596/jotcsa.1819206

Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents

Abstract

A new ligand of Mannich base (5-chloro-3-(dibenzyl amino) (phenyl)methyl) benzo[d]oxazole-2(3H)-thione) (L₁) was synthesized. Micro complexes (CuL₁) and (ZnL₁) were synthesized, and their anti-tumor and antioxidant activity against cancer cells (MCF-7) were investigated. The derivative (L₁) has been synthesized through condensation of 5-chloro-2-mercaptobenzoxazole, benzaldehyde with dibenzyl amine. Different techniques: FTIR, C.H.N.S., ¹H-NMR, UV-Vis, ¹³C-NMR, and mass spectral analysis were used to characterize the prepared ligand. UV-vis spectroscopy and FT-IR, flame atomic absorption technique, elemental analysis (C.H.N.S.), magnetic susceptibility, and conductivity measurements have been utilized for identifying and suggesting the geometrical structure of the synthesized complexes in the solid state. The DPPH assay was used to determine the free radical scavenging properties of the free ligand (L1) as well as its microcomplexes. The in vitro anticancer activity of each compound against breast cancer cells was evaluated using various parameters. AO-EtBr dual staining confirmed that the anti-proliferative effects of these compounds result from the induction of cell death and apoptosis. Based on the study’s findings, micro complexes may have broad medical applications and offer a promising alternative to conventional chemotherapy.

Keywords

Thanks

The authors are grateful to the Department of Chemistry at the College of Science at Al-Mustansiriyah University for providing the facilities and constant encouragement for this work.

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Details

Primary Language

English

Subjects

Bioinorganic Chemistry

Journal Section

Research Article

Authors

Marwa Jassim Al-Muhamadawi ^*
0009-0003-7287-0590
Iraq

Rehab Abdul Mahdi Al-Hassani This is me
Iraq

Publication Date

February 25, 2026

Submission Date

November 6, 2025

Acceptance Date

January 25, 2026

Published in Issue

Year 2026 Number: 2026-1

DOI

https://doi.org/10.18596/jotcsa.1819206

IZ

https://izlik.org/JA47CX58ZA

Cite

RIS / Bibtex

APA

Jassim Al-Muhamadawi, M., & Al-Hassani, R. A. M. (2026). Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents. Journal of the Turkish Chemical Society Section A: Chemistry, 2026-1, 1-16. https://doi.org/10.18596/jotcsa.1819206

AMA

1.Jassim Al-Muhamadawi M, Al-Hassani RAM. Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents. JOTCSA. 2026;(2026-1):1-16. doi:10.18596/jotcsa.1819206

Chicago

Jassim Al-Muhamadawi, Marwa, and Rehab Abdul Mahdi Al-Hassani. 2026. “Novel Mannich Base Complexes of 5-Chloro-1,3-Benzoxazole-2(3H)-Thione As Antioxidant and Anticancer Agents”. Journal of the Turkish Chemical Society Section A: Chemistry, no. 2026-1: 1-16. https://doi.org/10.18596/jotcsa.1819206.

EndNote

Jassim Al-Muhamadawi M, Al-Hassani RAM (February 1, 2026) Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents. Journal of the Turkish Chemical Society Section A: Chemistry 2026-1 1–16.

IEEE

[1]M. Jassim Al-Muhamadawi and R. A. M. Al-Hassani, “Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents”, JOTCSA, no. 2026-1, pp. 1–16, Feb. 2026, doi: 10.18596/jotcsa.1819206.

ISNAD

Jassim Al-Muhamadawi, Marwa - Al-Hassani, Rehab Abdul Mahdi. “Novel Mannich Base Complexes of 5-Chloro-1,3-Benzoxazole-2(3H)-Thione As Antioxidant and Anticancer Agents”. Journal of the Turkish Chemical Society Section A: Chemistry. 2026-1 (February 1, 2026): 1-16. https://doi.org/10.18596/jotcsa.1819206.

JAMA

1.Jassim Al-Muhamadawi M, Al-Hassani RAM. Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents. JOTCSA. 2026;:1–16.

MLA

Jassim Al-Muhamadawi, Marwa, and Rehab Abdul Mahdi Al-Hassani. “Novel Mannich Base Complexes of 5-Chloro-1,3-Benzoxazole-2(3H)-Thione As Antioxidant and Anticancer Agents”. Journal of the Turkish Chemical Society Section A: Chemistry, no. 2026-1, Feb. 2026, pp. 1-16, doi:10.18596/jotcsa.1819206.

Vancouver

1.Marwa Jassim Al-Muhamadawi, Rehab Abdul Mahdi Al-Hassani. Novel Mannich Base Complexes of 5-Chloro-1,3-benzoxazole-2(3H)-thione as Antioxidant and Anticancer Agents. JOTCSA. 2026 Feb. 1;(2026-1):1-16. doi:10.18596/jotcsa.1819206