Antibacterial activity of Phlomis fruticosa L. plant extracts

Year 2025, Volume: 1 Issue: 1, 28 - 31, 29.05.2025

Betül Budak Esra Yıldırım Servi

Abstract

Aim: This study aimed to determine the antibacterial activities of extracts obtained
from the Phlomis fruticosa plant.
Materials and methods: Petroleum ether, ethyl acetate, methanol, and
methanol:water (70:30) extracts were obtained from Phlomis fruticosa plant by the
Soxhlet method. The antibacterial activity of the extracts was determined using
the disc diffusion method. Antibacterial activity was started at a concentration of
50 mg/disc. Staphylococcus aureus, Bacillus subtilis, B. cereus, Escherichia coli and
Pseudomonas aeruginosa were used in the antibacterial activity tests.
Results: It was determined that the methanol extract showed the highest activity
on Pseudomonas aeruginosa bacteria among the four extracts by forming a zone
diameter of 25 mm. Other extracts were found to have inhibition zone diameters in
the 6-12 mm range against the tested bacteria.
Conclusion: In this study, the antibacterial activity of petroleum ether, ethyl acetate,
and methanol:water extracts of P. fruticosa was studied for the first time.
Since the methanol extract has a high inhibition zone diameter against P. aeruginosa
bacteria, the bioactive compounds responsible for this activity should be investigated
in future studies.

Keywords

Phlomis fruticosa , extract , antibacterial activity , the disc diffusion method , Soxhlet extraction

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