ELECTROCHEMICAL DETERMINATION OF THE INTERACTION BETWEEN ANTICANCER DRUG CAPECITABINE AND DNA BY CARBON PASTE ELECTRODE

Abstract

In this study, the interaction of an anticancer drug, Capecitabine with DNA was investigated by electrochemical methods using carbon paste electrode (CPE). The interaction of Capecitabine with single-stranded Calf thymus DNA (ss DNA) and double-stranded Calf thymus DNA (ds DNA) at electrode surface, or in solution phase was investigated by monitoring the changes at the oxidation signal of guanin base of DNA measured by differential pulse voltammetry (DPV) technique. Impedimetric measurements were performed by electrochemical impedance spectroscopy technique that also confirmed that ds DNA was immobilized onto the electrode surface. The detection limit (DL) in the case of interaction of Capecitabine with dsDNA and ssDNA interaction was calculated and found to be 17.35µg/mL and 17.12µg/mL, respectively.

Keywords

• DNA Biosensors,Capecitabine,CPE

References

1. ÖZSÖZ. M., ERDEM. A., KARA. P., KERMAN. K., ÖZKAN. D., Electrochemical biosensor for the detection of interaction between arsenic trioxide and DNA based on guanine signal, Electroanal., 15 (2002) 613- 619.
2. WANG. J., Electrochemical DNA biosensors. Electroanalytical methods for biological materials, (2002) 27–41
3. WANG. J., DNA electrochemical biosensors for environmental monitoring. A Review, Anal. Chim.Acta, 347 (1997) 1-9.
4. ERDEM. A., A review: Nanomaterial-based electrochemical DNA sensing strategies, Talanta, 74 (2007) 318-325
5. NAWAZ. H., RAUF. S., AKHTAR. K., KHALID. A.M., Electrochemical DNA biosensor for the study of ciprofloxacin–DNA interaction, Analytical Biochemistry, 354 (2006) 28–34.
6. RAUF. S., GOODING. J.J, AKHTAR. K., GHAURI. M.A, RAHMAN. M., ANWAR. M.A., KHALID. A.M., Electrochemical approach of anticancer drugs–DNA interaction, Journal of Pharmaceutical and Biomedical Analysis, 37 (2005) 205–217.
7. ERDEM. A., ÖZSÖZ. M., İnteraction of anticancer drug, Epirubicin with DNA, Anal. Chim. Acta, 437 (2001) 107-114
8. WANG. J., RIVAS. G., FERNANDES. J. R., JIANG. M., PAZ. J.L.L., WAYMIRE. R., NIELSEN. T.W., GETTS. R.C., Adsorption and detection of DNA dendrimers at carbon electrodes, Electroanal., 10 (1998) 553-556.

9. MIKKELSEN. S. R., Electrochemical biosensors for DNA sequence detection- a review, Electroanal.,8 (1996) 15-19.
10. WANG. L., LIN. L., YE. B., Electrochemical studies of the interaction of the anticancer herbal drug emodin with DNA, Journal of Pharmaceutical and Biomedical Analysis, 42 (2006) 625–629.
11. NIU. S., ZHAO. M., HU. L., ZHANG. Z., Carbon nanotube-enhanced DNA biosensor for DNA hybridization detection using rutin-Mn as electrochemical indicator, Sensors and Actuators B, 135 (2008) 200-205.
12. RICHARDSON. C.L., G.E.S. Springfield, "Intercalation inhibition assay compounds that interact with DNA or RNA", Amerikan patent (1981), no; 4,257,774 / 24 March.
13. MARIN. D., PEREZ. P., TEIJEIRO. C., PALECEK. E., Interactions of surface-confied DNA with acid-activated mitomycin C, Biophysical Chemistry, 75 (1998) 87-95.
14. PALECEK. E., From Polarography of DNA to Microanalysis with Nucleic Acid Modified Electrodes, Electroanal, 8 (1996) 7-14.
15. WANG. J., CAI. X., RIVAS. G., SHIRAISHI. H., Stripping potentiometric transduction of DNA hybridization processes, Anal. Chim. Acta, 326 (1996) 141-147.
16. YAN. F., ERDEM. A., MERİC. B., KERMAN. K., ÖZSÖZ. M., SADİK. O. A., Electrochemical DNA biosensor for the detection of specific gene related to Microcystis species, Electrochemistry Communications, 3 (2001) 224-228.
17. KARADENİZ. H., ERDEM. A., CALISKAN. A., PEREIRA. C. M., PEREIRA. E. M., RİBİERO. J. A., Electrochemical sensing of silver tags labelled DNA immobilized onto disposable graphite electrodes, Electrochem. Commun, 9 (2007) 2167-2173.
18. ERDEM. A., KERMAN. K., MERİÇ. B., AKARCA. U. S., ÖZSÖZ. M., DNA electrochemical biosensor for the detection of short DNA sequences related to the hepatitis B virus, Electroanal, 11(1999) 586-588.
19. WANG. J., KAWDE. A. N., ERDEM. A., SALAZAR. M., Magnetic beadbased label-free electrochemical detection of DNA hybridization, Analyst, 126 (2001) 2020-2024.
20. KURALAY. F., ERDEM. A., ABACI. S., OZYORUK. H., YILDIZ. A., Characterization of redox polymer based electrode and electrochemical behavior for DNA detection, Analytica Chimica Acta, 643 (2009) 83-89.