HISTOPATHOLOGICAL CHANGES IN THE STOMACH MUCOSA OF RATS FED WITH GREAT SCALLOP (PECTEN MAXIMUS)

Year 2018, Volume: 2 Issue: 3, 105 - 114, 31.07.2018

Aslı Muratlı Mehmet Rıza Gezen

Abstract

Heavy metals, industrial and household wastes and
pesticides are threats for the aquatic ecosystem. Polluted water sources are
streaming into the seas and cause pollution in these systems. Dardanelles is
exposed to pollution from the Marmara and Black Sea. Our previous studies
demonstrated that the water and mollusc from certain regions of the Dardanelles
contained heavy metal salts. The purpose of the study is to demonstrate the
histopathologic changes in the gastric tissues of rats which are fed with great
scallop (Pecten maximus) that are collected from the Çardak region of the
Dardanelles. Four groups of rats are included in the study, group 1 (n=6),
control group fed with standard rat food, group 2 (n=6), 75%  great scallop and 25% standard rat food
daily, group 3 (n=6), 75% great scallop and 25% standard rat food every two
days, group 4 (n=6), 75%  great scallop
and 25% standard rat food every three days. After the routine histopathologic
processing all gastric tissue samples are evaluated in terms of 8-hydroxy-2'
-deoxyguanosine (8-OHdG) immunoreactivity with light microscopy and image
analysis software. No histopathologic differences found in standard hematoxylin-eosin
(H.E.) stained gastric tissue samples of the control group. Second group showed
active chronic gastritis, third group showed less inflamation and chronic
gastritis compared with the second group and fourth group showed less
mononuclear inflammation compared to the second and third groups. In
immunohistochemical staining, 8-OHdG immunoreactivity in gastric epithelial
cells. 8-OHdG immunoreactivity was negative in stomach tissues in all
groups.  There was no statistically
significant difference between the groups that were fed every day, every other
day and every three days with great scallop (p>0.05). The results of our
studies showed that rats fed more with great scallops could produce gastritis
in the stomach.

Keywords

Dardanelles, 8-hydroxy-2' -deoxyguanosine, immunohistochemistry, stomach, great scallop

References

• REFERENCES
• ANONYMOUS, 2002, Fisheries Low and Regulations. Turkey Ministry of Agriculture and Rural Affairs Press, Ankara, Turkey. ATES, I., SUZEN, H.S., AYDIN, A., KARAKAYA, A., 2004, The Oxidative DNA Base Damage In Testes Of Rats After Intraperitoneal Cadmium Injection, Biometals, 17(4), 371-7. AVUNDUK, M.C., TAVLI, S., YOL, S., TAVLI, L., YAVUZ, A., GUNGOR, S., YILMAZ, O., 2000, Mide karsinomlarında hücre proliferasyon belirleyicisi olarak PCNA, Ki 67 ve agNOR kullanımı, Ankara Üniversitesi Tıp Fakültesi Mecmuası, 53(1), 11-15. BAE, Y.S., KANG, S.W., SEO, M.S., 1997, Epidermal growth factor (EGF)-induced generation of hydrogen peroxide, J Biol Chem, 272, 217–21. BAKIR, K., KAPUCUOGLU, N., BULBUL, H.D., 1996, PCNA (Proliferating Cell Nuclear Antigene) immunohistokimyasal boyanması ve agNORs’un meme karsinomunda prognozu belirlemedeki rolü, Türk Patholoji Dergisi, 12(1), 35-38. BALKIS, N., ALGAN, O., 2005, Marmara Denizi yüzey sedimentlerinde metallerin birikimi ve denetleyen mekanizmalar, Deniz Kirliliği, 21, TÜDAV Yayınları, İstanbul. BURCAK, G., ANDICAN, G., 2004, Oksidatif DNA Hasarı ve Yaşlanma., Cerrahpasa J Med, 35(4), 159-169. CHOPRA, S., WALLACE, H.M., 1998, Induction of spermidine/ spermine N1-acetyltransferase in human cancer cells in response to increased production of reactive oxygen species. Biochem Pharmacol, 55, 1119–23. CLARKSON, T.W., 2002, The three modern faces of mercury, Environ Health Perspect, 110,11-23. CZENE, S., TIBA, C.K.M, HARMS-RINGDAHL, M., 1997, pH-dependent DNA cleavage in permeabilized human fibroblasts, Biochem J, 323, 337–41. DEMIR, N., GEZEN, MR., BALLI, M., 2011, Çanakkale Boğazı’nın Karacaören Kıyısındaki Deniz Suyu ve Bazı Yumuşakçalarda (Bivalvia ve Gastropoda) Ağır Metal Düzeylerinin Araştırılması, Ekoloji Sempozyumu, 5-7 Mayıs Düzce, 177. FRAGA, C.G., SHINENAGA, M.K., PARK, J.W., DEGAN, P., AMES, B.N., 1990, Oxidative Damage to DNA During Aging: 8-hydroxy-2’-deoxyguanosine in Rat Organ DNA and Urine, Proc Natl Acad Sci USA, 87(12), 4533-7. GEZEN, M.R., DEMIR, N., CETIN, M., 2011, Çanakkale Boğazı’nın Umurbey Kıyısındaki Deniz suyu ve Bazı Yumuşakçalarda (Bivalvia ve Gastropoda) Ağır Metal Düzeylerinin Araştırılması, Ekoloji 2011 Sempozyumu, 5-7 Mayıs Düzce, 178. GEZEN, M.R., DEMIR, N., CETIN, M., BALLI, M., 2011, Çanakkale Boğazı’ndaki Deniz Kestanesi (Paracentrotus lividus)’ nin Ağır Metal Düzeyleri Üzerinde Bir Araştırma, Ekoloji Sempozyumu, 5-7 Mayıs Düzce, 176. GEZEN, M.R., 2017, Histopathological Research and Diagnosis of Important Paiting Methods, JSP, 1(1), 23-27. GHOSH, J., MYERS, C.E., 1998, Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells, Proc Natl Acad Sci, 95, 13182–7. HALLIWELL, B., 2000, Why and How Should We Measure Oxidative DNA Damage in Nutritional Studies? How Far Have We Come? Am J Clin Nutr, 72(5), 1082-7. HURST, R., BAO, Y., JEMTH, P., MANNERVIK, B., WILLIAMSON, G., 1997, Phospholipid hydroperoxide glutathione peroxidase activity of rat class Theta glutathione transferase T2-2, Biochem Soc Trans, 25(4), 559. IARC (International Agency for Research on Cancer), 1987, Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans: Arsenic and Arsenic Compounds (Group 1), vol. 7, World Health Organization, Lyon, France, 100–106. JORNOT, L., PETERSEN, H., JUNOD, A.F., 1998, Hydrogen peroxide induced DNA damage is independent of nuclear calcium but dependent on redox-active ions, Biochem J, 335, 85–94. KARA, H., COLAKOĞLU, N., KUKNER, A., OZAN, E., 2004, Structural changes of cadmium chloride that occurs in tissue of rat and effects of Metallothionein on these changes light: Microscobic work, J Med Sci, 24, 6. LEE, Y.J., GALOFORO, S.S., BERNS, C.M., CHEN, J.C., DAVIS, B.H., SIM, J.E., CORRY, P.M., SPITZ, D.R., 1998, Glucose deprivation-induced cytotoxicity and alterations in mitogen-activated protein kinase activation are mediated by oxidative stress in multidrug-resistant human breast carcinoma cells, J Biol Chem, 273, 5294–9. MANTOVANI, A., ALLAVENA, P., SICA, A., BALKWILL, F., 2008, Cancer-related İnflammation, Nature, 454, 436-44. NIEBOER, E., TSUJI, L.J., MARTIN, I.D., LIBERDA, EN., 2013, Human biomonitoring issues related to lead exposure. Environ Sci Process Impacts, 15, 1824-9. OZKURNAZ, G., DEMIR, NÇ, 2012, Umurbey Bölgesi’nden (Çanakkale) Toplanan Mytilus galloprovincialis ve Pecten maximus Dokularında Ağır Metal Birikiminin İncelenmesi, 21. Ulusal Biyoloji Kongresi, 3-7 Eylül İzmir, 880-881. SIOMEK, A., TUJAKOWSKI, J., GACKOWSKI, D., ROZALSKI, R., FOKSINSKI, M., DZIAMAN, T., ROSZKOWSKI, K., OLINSKI, R., 2006, Severe oxidatively damaged DNA after cisplatin treatment of cancer patients, Int J Cancer, 119(9), 2228-30. TOPCUOĞLU, S., GÜVEN, K.C., BALKIS, N., KIRBASOĞLU, C., 2003, Heavy metal monitoring of marine algae from the Turkish coast of the Black Sea, 1998-2000, Chemosphere, 52, 1683-1688. TOSUN, E., TOSUN, M., AVUNDUK, M.C., 2006, Hücre çoğalma belirleyicilerinin meme infiltratif duktal karsinomlarında progrostik amaçlı kullanımı, Optimal Tıp Dergisi, 19(2), 27-40. TUZEN, M., 2003, Determination of heavy metals in fish samples of middle Black Sea (Turkey) by graphite furnace atomic absorption spectrometry, Food Chem, 80, 119-123. WILLIAMS, G.M., JEFFREY, A.M., 2000. Oxidative DNA Damage: Endogenous and Chemically Induced, Regul Toxicol Pharmacol, 32(3), 283-92. WOJTASZEK, P., 1997, Oxidative burst: an early plant response to pathogen infection. Biochem J, 322, 681–92. YIN, G.Y., YIN, Y.F., HE X.F., 1995, Effect of zhuchun pill on immunity and endocrine function of elderly with kidney-yang deficiency. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih, 15, 601–3.