Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections

Ayşe Karacalı Tunç; Togrul Nagiyev; Tülay Kandemir; Melda Meral Öcal; Fatih Köksal

Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections

Abstract

Aim: Acinetobacter baumannii is an important pathogen causing concern worldwide with increasing antibiotic resistance. We aimed to provide epidemiological data and molecular differences between multidrug resistant A.baumannii isolates obtained from 7 different centers in Türkiye. Material and Method: One hundred sixty-six multidrug-resistant A.baumannii strains were included in the study. All the isolates were tested for antibiotic susceptibility according to EUCAST criteria. To molecularly confirm the carbapenem-resistant A.baumannii isolates, the specific blaOXA-51-like gene region was detected by polymerase chain reaction (PCR) method. A possible phylogenetic relationship between multi-drug resistant isolates was investigated using Pulsed-field Gel Electrophoresis method. Results: One hundred sixty-six carbapenem-resistant A.baumannii strains were evaluated by the ApaI-PFGE (Pulsed-field gel electrophoresis –PFGE) method using the CHEF-DRII electrophoresis system. A total of 51 clusters were grouped into 142 pulsotypes. Twenty clusters had single members. Thirty-one clusters had multiple members and were identified with the capital letters of the alphabet such as A, B, …, and Z26. It was observed that 40 PFGE profiles showed 100% related. Conclusion: More intense contamination is in question in the provinces with many patients. In four of the seven different centers, more innocent results were obtained in terms of the number of samples, antibiotic resistance and clonal relationship when compared to the other three centers. However, this may be caused by the low population zone and inadequate health services. Regional or multicenter studies are minimal. Thus, further studies involving more centers and larger populations are needed. Consequently, the present study will shed light on future studies and create thriving literature on border regions

Keywords

References

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Details

Primary Language

English

Subjects

Clinical Microbiology

Journal Section

Research Article

Authors

Ayşe Karacalı Tunç This is me
Türkiye

Togrul Nagiyev This is me
Türkiye

Tülay Kandemir This is me
Türkiye

Melda Meral Öcal This is me
Türkiye

Fatih Köksal This is me
Türkiye

Early Pub Date

September 15, 2023

Publication Date

August 25, 2023

Submission Date

April 9, 2023

Acceptance Date

May 18, 2023

Published in Issue

Year 2023 Volume: 13 Number: 2

IZ

https://izlik.org/JA36MU25KW

Cite

RIS / Bibtex

APA

Karacalı Tunç, A., Nagiyev, T., Kandemir, T., Öcal, M. M., & Köksal, F. (2023). Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections. Kafkas Journal of Medical Sciences, 13(2), 197-205. https://izlik.org/JA36MU25KW

AMA

1.Karacalı Tunç A, Nagiyev T, Kandemir T, Öcal MM, Köksal F. Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections. Kafkas Journal of Medical Sciences. 2023;13(2):197-205. https://izlik.org/JA36MU25KW

Chicago

Karacalı Tunç, Ayşe, Togrul Nagiyev, Tülay Kandemir, Melda Meral Öcal, and Fatih Köksal. 2023. “Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter Baumannii Infections”. Kafkas Journal of Medical Sciences 13 (2): 197-205. https://izlik.org/JA36MU25KW.

EndNote

Karacalı Tunç A, Nagiyev T, Kandemir T, Öcal MM, Köksal F (August 1, 2023) Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections. Kafkas Journal of Medical Sciences 13 2 197–205.

IEEE

[1]A. Karacalı Tunç, T. Nagiyev, T. Kandemir, M. M. Öcal, and F. Köksal, “Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections”, Kafkas Journal of Medical Sciences, vol. 13, no. 2, pp. 197–205, Aug. 2023, [Online]. Available: https://izlik.org/JA36MU25KW

ISNAD

Karacalı Tunç, Ayşe - Nagiyev, Togrul - Kandemir, Tülay - Öcal, Melda Meral - Köksal, Fatih. “Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter Baumannii Infections”. Kafkas Journal of Medical Sciences 13/2 (August 1, 2023): 197-205. https://izlik.org/JA36MU25KW.

JAMA

1.Karacalı Tunç A, Nagiyev T, Kandemir T, Öcal MM, Köksal F. Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections. Kafkas Journal of Medical Sciences. 2023;13:197–205.

MLA

Karacalı Tunç, Ayşe, et al. “Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter Baumannii Infections”. Kafkas Journal of Medical Sciences, vol. 13, no. 2, Aug. 2023, pp. 197-05, https://izlik.org/JA36MU25KW.

Vancouver

1.Ayşe Karacalı Tunç, Togrul Nagiyev, Tülay Kandemir, Melda Meral Öcal, Fatih Köksal. Multicenter Research of the Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Infections. Kafkas Journal of Medical Sciences [Internet]. 2023 Aug. 1;13(2):197-205. Available from: https://izlik.org/JA36MU25KW