Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri

Enes Yıldırım; Zehra Alkan; Ahmet Regaib Oğuz

doi:10.31466/kfbd.1600454

EN TR

The Histological Effects of Salt Stress on the Digestive Tract of Pseudotropheus acei Fish

Abstract

The digestive tract is responsible for food digestion in fish, as it is in all living organisms. Feeding habits, canal structure, and morphology of fish vary among species. The mucous cells in the mucosal layer of the digestive tract are influenced by factors such as salinity, ion regulation, pH, pollutants, parasites, and stress. Salinity is one of the most important factors affecting fish growth, nutrition, metabolism, and other physiological activities. In our experimental study, different concentrations of salt were applied to Pseudotropheus acei fish. It was observed that the fish adapted to a maximum salinity of 27 ppt and died at higher salinities. The digestive tract tissues of both control and salinity-adapted fish were examined histologically. The histological studies revealed that the digestive tract of Pseudotropheus acei consisted of four layers: serous, muscularis, submucosa, and mucosa. Mucus cells in the digestive tract of fish exposed to salt stress were counted from 15 different regions and statistically analyzed. Statistical analysis revealed that the number of mucus cells containing neutral glycoconjugates was 267.4 in the control group, 328.66 in the 20 ppt group, and 360 in the 27 ppt group. The densities of neutral, carboxylated, O-sulfate ester, strongly sulfated, and sulfated glycoconjugates in the mucus cells, important cells within the digestive tract, were determined histochemically using Periodic Acid/Schiff (PAS), Alcian Blue at pH 2.5, Alcian Blue at pH 1.0, Alcian Blue at pH 0.5, and Aldehyde Fuchsin stains. It was concluded that salinity stress had a significant effect on the mucus cell densities and content in fish living in aquatic environments.

Keywords

Histology, Mucus Cell, Digestive tract, Salinity

Project Number

1919B012213680

Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri

Abstract

Sindirim kanalı tüm canlılarda olduğu gibi balıklarda da besinlerin sindiriminden sorumlu organdır. Balıkların beslenme şekli, kanal yapısı ve morfolojisi türden türe farklılık göstermektedir. Balıklarda sindirim kanalı mukoza tabakasında bulunan mukus hücreleri tuzluluk, iyon regülasyonu, pH, kirletici, parazit ve stres gibi faktörlerden etkilenmektedir. Tuzluluk balık büyümesi, beslenmesi, metabolizması ve diğer fizyolojik aktiviteleri etkileyen en önemli parametrelerden biridir. Bu çalışmada Pseudotropheus acei balıklarına farklı tuz konsantrasyonları uygulandı. Uygulama sonucunda balıkların en fazla 27 ppt tuzluluğa adapte olduğu daha yüksek tuzluluklarda ise öldüğü gözlendi. Kontrol ve tuzluluğa adapte olan gruplardaki balıkların sindirim kanalları histolojik olarak incelendi. Yapılan çalışmalar sonucunda Pseudotropheus acei balığı sindirim kanalının seröz, muskularis, submukoza ve mukoza tabakalarından oluştuğu görüldü. Tuz stresine maruz kalan balıkların sindirim kanalı mukus hücreleri 15 farklı bölgeden sayılarak istatistiksel olarak analiz edilmiştir. İstatiksel analiz sonucu, nötral glikokonjugat içeren mukus hücre sayısının kontrol grubunda 267.4, 20 ppt grubunda 328.66 ve 27 ppt grubunda 360 olduğu görüldü. Sindirim kanalında bulunan önemli hücrelerden biri olan mukus hücrelerinin nötral, karboksilatlı, O-sülfat esterli, güçlü sülfatlı ve sülfatlanmış glikokonjugat yoğunlukları Periyodik Asit/Schiff (PAS), Alcian Blue pH 2.5, Alcian Blue pH 1.0, Alcian Blue pH 0.5 ve Aldehit fuksin&Alcian Blue pH 2.5 boyaları ile histokimyasal olarak belirlendi. Tuzluluk stresinin balıklarda sindirim kanalında bulunan mukus hücre yoğunlukları ve içeriği üzerine etkili olduğu görüldü.

Keywords

Histoloji, Mukus Hücresi, Sindirim Kanalı, Tuzluluk

Supporting Institution

TÜBİTAK 2209-A üniversite öğrencileri araştırma destek programı

Project Number

1919B012213680

Ethical Statement

Van Yüzüncü Yıl Üniversitesi Hayvan Araştırmacıları Yerel Etik Kurulu (YUHADYEK 2023/05-36)

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Details

Primary Language

Turkish

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Enes Yıldırım
0009-0005-1465-5037
Türkiye

Zehra Alkan ^*
0000-0003-2591-0839
Türkiye

Ahmet Regaib Oğuz
0000-0001-6431-0508
Türkiye

Publication Date

December 15, 2025

Submission Date

December 12, 2024

Acceptance Date

December 5, 2025

Published in Issue

Year 2025 Volume: 15 Number: 4

DOI

https://doi.org/10.31466/kfbd.1600454

IZ

https://izlik.org/JA23BM26KC

APA

Yıldırım, E., Alkan, Z., & Oğuz, A. R. (2025). Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri. Karadeniz Fen Bilimleri Dergisi, 15(4), 1472-1489. https://doi.org/10.31466/kfbd.1600454

AMA

1.Yıldırım E, Alkan Z, Oğuz AR. Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri. KFBD. 2025;15(4):1472-1489. doi:10.31466/kfbd.1600454

Chicago

Yıldırım, Enes, Zehra Alkan, and Ahmet Regaib Oğuz. 2025. “Tuz Stresinin Pseudotropheus Acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri”. Karadeniz Fen Bilimleri Dergisi 15 (4): 1472-89. https://doi.org/10.31466/kfbd.1600454.

EndNote

Yıldırım E, Alkan Z, Oğuz AR (December 1, 2025) Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri. Karadeniz Fen Bilimleri Dergisi 15 4 1472–1489.

IEEE

[1]E. Yıldırım, Z. Alkan, and A. R. Oğuz, “Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri”, KFBD, vol. 15, no. 4, pp. 1472–1489, Dec. 2025, doi: 10.31466/kfbd.1600454.

ISNAD

Yıldırım, Enes - Alkan, Zehra - Oğuz, Ahmet Regaib. “Tuz Stresinin Pseudotropheus Acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri”. Karadeniz Fen Bilimleri Dergisi 15/4 (December 1, 2025): 1472-1489. https://doi.org/10.31466/kfbd.1600454.

JAMA

1.Yıldırım E, Alkan Z, Oğuz AR. Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri. KFBD. 2025;15:1472–1489.

MLA

Yıldırım, Enes, et al. “Tuz Stresinin Pseudotropheus Acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri”. Karadeniz Fen Bilimleri Dergisi, vol. 15, no. 4, Dec. 2025, pp. 1472-89, doi:10.31466/kfbd.1600454.

Vancouver

1.Enes Yıldırım, Zehra Alkan, Ahmet Regaib Oğuz. Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri. KFBD. 2025 Dec. 1;15(4):1472-89. doi:10.31466/kfbd.1600454

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The Histological Effects of Salt Stress on the Digestive Tract of Pseudotropheus acei Fish

Abstract

Keywords

Project Number

Tuz Stresinin Pseudotropheus acei Balığının Sindirim Kanalı Üzerindeki Histolojik Etkileri

Abstract

Keywords

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Ethical Statement

References

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IZ

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