Vişne Sapı Ekstraktlarının in vitro Metotlarla Antioksidan Aktivitelerinin Belirlenmesi

Year 2020, Volume: 10 Issue: 2, 290 - 301, 15.12.2020

Filiz Uçan Türkmen , Hatice Aysun Mercimek Takcı , Fatma Esen Sarıgüllü Önalan

https://doi.org/10.31466/kfbd.722885

Cited By: 8

https://izlik.org/JA68NJ89BR

Abstract

Bu çalışmada, vişne (Prunus cerasus L.) sapı ekstrelerinin antioksidan aktiviteleri β-karoten/linoleik asit, metal şelatlama, güç azaltma, DPPH radikal süpürme, ABTS yöntemi ve TEAC metodu kullanılarak araştırılmıştır. Bu amaçla, saplar kurutulduktan sonra damıtılmış su, metanol, etil asetat ve hekzan çözücüleri kullanılarak ayrı ayrı özütlenmiş ve ince toz haline getirilmiştir. Farklı konsantrasyonlarda (100-1000 μg/mL) hazırlanan ekstrelerin antioksidan aktiviteleri a-tokoferol, askorbik asit, BHT ve BHA standartları ile karşılaştırılmıştır. Vişne sapı metanol ekstresininen yüksek verime (% 22) sahip olduğu belirlenmiştir. 100-1000 µg/mL konsantrasyonlarda metanol ekstresinin şelatlama aktiviteleri (Fe2+)%18.45 ile 67.75 arasında değişmektedir. Bununla birlikte, tüm test ekstrelerinin kenetleme aktiviteleri standartlardan daha düşük olmuştur. Hekzan hariç tüm ekstrelerin indirgeme gücü konsantrasyondaki artışa bağlı olarak artmıştır. Metanol ekstresinin DPPH radikal temizleme etkinliği,%14.57 ila85.78 arasında değişmiştir. En düşük EC50 değeri, metanol ekstresiiçin 0.494 µg/mL olarak belirlenmiştir. Ancak, linoleik asit oksidasyonunun en yüksek yüzde inhibisyonu, hekzan ekstresinde(% 57.22) bulunmuştur. Metanol ekstresinin Trolox eşdeğeri antioksidan kapasite değerleri 15.43 ila 20.54 µM Troloxeşdeğeri /10 g arasında değişmektedir. Metanol ekstresinin antioksidan aktiviteler üzerinde doza bağlı bir inhibisyon gösterdiği belirlenmiştir.

Keywords

Şifalı bitkiler , Antioksidan aktivite , β-karoten/linoleik asit , Şelatlama , Vişne sapı , Güç azaltma

Project Number

11932

Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods

https://izlik.org/JA68NJ89BR

Abstract

In this study, the antioxidant activities of sour cherry (Prunus cerasus L.) stalk extracts were investigated using β-caroten/linoleic acid, metal chelating, reducing power, DPPH radical scavenging, ABTS method and TEAC method. For this purpose the stalks were extracted separately by using distilled water, methanol, ethyl acetate and hexane solvents following dried and ground to fine powder. The antioxidant activities of extracts prepared at the different concentration (100-1000 μg/mL) were compared with α-tocopherol, ascorbic acid, BHT and BHA standards. The methanol extract of stalk had the highest yield (22%). Chelating activities (Fe2+) of methanol extract at 100-1000 μg/mL concentrations ranged between 18.45 and 67.75%. However, chelating activities of all test extracts were found lower than standards. The reducing power of all extracts except hexane increased depending upon the increase in concentration. DPPH radical scavenging activity of methanol extract varied in the range of 14.57 to 85.78%. The lowest EC50 value was determined as 0.494 µg/mL for methanol extract. But, the highest inhibition percentage of linoleic acid oxidation was found in hexane extract (57.22%). TEAC values of methanol extract ranged from 15.43 to 20.54 µM Trolox equivalent/10 g. It was determined that methanol extract showed a dose-dependent inhibition on the antioxidant activities.

Keywords

Antioxidant activity , Medicinal plants , Β-Caroten/Linoleic acid , Chelating , Cherry stalk , Reducing power

Supporting Institution

Kilis 7 Aralık Üniversitesi BAP birimi

Project Number

11932

Thanks

This study was supported by Kilis 7 Aralık University Research Fund (Project number: 11932).

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