Research Article
BibTex RIS Cite

Streptomyces sp. K22 ve K30 Suşlarından Lipaz ve Proteaz Enzim Üretimi

Year 2017, Volume: 7 Issue: 2, 128 - 135, 15.12.2017
https://doi.org/10.31466/kfbd.343293

Abstract

Mikrobiyal enzimler önemli bir biyoteknolojik
araçlardır. Birçok biyolojik ve kimyasal reaksiyonda rol almaktadırlar ve
çeşitli endüstrilerdeki farklı alanlarda kendilerine önemli sayılabilecek bir
yer edinmişlerdir. Mikrobiyal enzimler arasından lipaz ve proteazlar, bu grubun
önemli birer üyeleridirler. Dolayısıyla, bu enzimleri üreten
mikroorganizmaların seçimi ve ürettikleri enzimlerin karakteristiklerinin
saptanması, pek çok araştırmacıyı bu alanda çalışmaya teşvik etmektedir. Bu
çalışmada, Karadeniz deniz sediment örneklerinden izole edilmiş aktinobakteri
suşlarının hücre dışı lipaz ve proteaz enzim üretim kapasitelerinin
belirlenmesi ve pH ile sıcaklık kararlılıklarının saptanması amaçlanmıştır.
Çalışmadan elde edilen sonuçlar değerlendirildiğinde, bu organizmaların iyi
birer lipaz ve proteaz enzim üreticisi ve bunların enzimlerinin geniş birer pH
ve sıcaklık kararlılıklarına sahip oldukları saptanmıştır

References

  • Aehle, W., (2007). Enzymes in Food Applications, Enzymes In Industry – Production and Applications. Wiley-Verlag. Bradford, M., (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254.
  • Ferrero, M.A., Castro, G.R., Abate, C.M., Baigori, M.D., Sineriz, F., (1996). Thermostable alkaline proteases of Bacillus licheniformis MIR 29: Isolation, production and characterization. Applied Microbiology and Biotechnology, 45, 327.
  • Garcia-Sanchez, A., Cerrato, R., Larrasa, J., Ambrose, N.C., Parra, A., Alonso, J.M., Hermoso-de-Mendoza, M., Rey, J.M., Mine, M.O., Carnegie, P.R., Ellis, T.M., Masters, A.M., Pemberton, A.D., Hermoso-de-Mendoza, J., (2004). Characterisation of an extracellualr serine protease gene (nasp gene) from Dermatophilus congolensis. FEMS Microbiology Letters, 231: 53-57.
  • Ghorbel, S., Kammoun, M., Soltana, H., Nasri, M., Hmidet, N., (2014). Streptomyces flavogriseus HS1: Isolation and characterization of extracellular proteases and their compatibility with laundry detergents. BioMed Research International, 2014, 1-8.
  • Godtfredsen, S.E., (1990). Microbial lipases. In W.M. Fogarty and E.T. Kelly (Eds.), Microbial Enzymes and Biotechnology (pp. 255-274). Elsevier: Amsterdam. Goodfellow, M., Williams, S.T., (1983). Ecology of actinomycetes. Annual Review of Microbiology, 37, 189-216.
  • Izrael-Zivkovic, L., Gojgic-Cvijovic, G., Karadzic, I., (2010). Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. Journal of the Serbian Chemical Society, 75(8), 1041-1052.
  • Kumar, C.G., Takagi, H., (1999). Microbial alkaline protease; from a bio industrial view point. Biotechnology Advances, 17, 561-594.
  • Kumar, S., Kikon, K., Upadhyay, A., Kanwar, S.S., Gupta, R., (2005). Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expression and Purification, 41, 38-44.
  • Maal, K.B., Emtiazi, G., Nahvi, I., (2009). Production of alkaline protease by Bacillus cereus and Bacillus polymixa in new industrial culture mediums and its immobilization. African Journal of Microbiology Research, 3, 491.
  • Mehrotra, S., Pandey, P.K., Gaur, R., Darmwal, N.S., (1999). The production of alkaline protease by a Bacillus species isolate. Bioresource Technology, 67, 201-203.
  • Mobarak-Qamsari, E., Kasra-Kermanshahi, R., Moosavi-Nejad, Z., (2011). Isolation and identification of a novel, lipase-producing bacterium, Pseudomnas aeruginosa KM110. Iranian Journal of Microbiology, 3(2), 92-98.
  • Oberoi, R., Beg, Q.K., Puri, S., Saxena, R.K., Gupta, R., (2001). Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp. World Journal of Microbiology&Biotechnology, 17, 493-497.
  • Sangeetha, R., Geetha, A., Arulpandi, I., (2008). Optimization of protease and lipase production by Bacillus pumilus SG 2 isolated from an industrial effluent. The Internet Journal of Microbiology, 5(2), 1-8.
  • Rapp, P., Backhaus, S., (1992). Formation of extracellular lipase by filamentous fungi, yeasts and bacteria. Enzyme and Microbial Technology, 14, 938-943.
  • Rathi, P., Saxena, R.K., Gupta, R., (2001). A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Process Biochemistry, 37, 187-192.
  • Remya, M., Vijayakumar, R., (2008). Isolation and characterization of marine antagonistic actinomycetes from west coast of India. Medicine and Biology, 15(1), 13-19.
  • Sztajer, H., Maliszewska, I., Wieczorek, J., (1988). Production of exogenous lipase by bacteria, fungi and actinomycetes. Enzyme and Microbial Technology, 10, 492-497.
  • Ülker, S., Özel, A., Çolak, A., Alpay Karaoğlu, Ş., (2011). Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil. Turkish Journal of Biology, 35, 543-550.
  • Wiseman, A., (1987). Handbook of Enzymes Biotechnology, Second Edition. Ellis Horwood Limited: Chichester.

Production of Lipase and Protease Enzymes from Streptomyces sp. K22 and K30 Strains

Year 2017, Volume: 7 Issue: 2, 128 - 135, 15.12.2017
https://doi.org/10.31466/kfbd.343293

Abstract

Microbial enzymes are important biotechnological
tools. They play role in several biological and chemical reactions and have
acquired a significant place in different fields in various industries. Among
microbial enzymes, lipases and proteases are important members of the group.
Therefore, the selection of microorganisms producing these enzymes and the
determination of their characteristics trigger researchers to work on this
field. In this study, it was aimed to investigate production capacities of
extracellular lipase and protease enzymes by actinobacteria strains isolated
from Black Sea sediment samples and to determine their pH and temperature
stabilities. In lights of the results obtained from the study, it was found that
these organisms are well-producer of lipase and protease enzymes, and their
enzymes have a wide range of pH and temperature stability.

References

  • Aehle, W., (2007). Enzymes in Food Applications, Enzymes In Industry – Production and Applications. Wiley-Verlag. Bradford, M., (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2), 248-254.
  • Ferrero, M.A., Castro, G.R., Abate, C.M., Baigori, M.D., Sineriz, F., (1996). Thermostable alkaline proteases of Bacillus licheniformis MIR 29: Isolation, production and characterization. Applied Microbiology and Biotechnology, 45, 327.
  • Garcia-Sanchez, A., Cerrato, R., Larrasa, J., Ambrose, N.C., Parra, A., Alonso, J.M., Hermoso-de-Mendoza, M., Rey, J.M., Mine, M.O., Carnegie, P.R., Ellis, T.M., Masters, A.M., Pemberton, A.D., Hermoso-de-Mendoza, J., (2004). Characterisation of an extracellualr serine protease gene (nasp gene) from Dermatophilus congolensis. FEMS Microbiology Letters, 231: 53-57.
  • Ghorbel, S., Kammoun, M., Soltana, H., Nasri, M., Hmidet, N., (2014). Streptomyces flavogriseus HS1: Isolation and characterization of extracellular proteases and their compatibility with laundry detergents. BioMed Research International, 2014, 1-8.
  • Godtfredsen, S.E., (1990). Microbial lipases. In W.M. Fogarty and E.T. Kelly (Eds.), Microbial Enzymes and Biotechnology (pp. 255-274). Elsevier: Amsterdam. Goodfellow, M., Williams, S.T., (1983). Ecology of actinomycetes. Annual Review of Microbiology, 37, 189-216.
  • Izrael-Zivkovic, L., Gojgic-Cvijovic, G., Karadzic, I., (2010). Isolation and partial characterization of protease from Pseudomonas aeruginosa ATCC 27853. Journal of the Serbian Chemical Society, 75(8), 1041-1052.
  • Kumar, C.G., Takagi, H., (1999). Microbial alkaline protease; from a bio industrial view point. Biotechnology Advances, 17, 561-594.
  • Kumar, S., Kikon, K., Upadhyay, A., Kanwar, S.S., Gupta, R., (2005). Production, purification, and characterization of lipase from thermophilic and alkaliphilic Bacillus coagulans BTS-3. Protein Expression and Purification, 41, 38-44.
  • Maal, K.B., Emtiazi, G., Nahvi, I., (2009). Production of alkaline protease by Bacillus cereus and Bacillus polymixa in new industrial culture mediums and its immobilization. African Journal of Microbiology Research, 3, 491.
  • Mehrotra, S., Pandey, P.K., Gaur, R., Darmwal, N.S., (1999). The production of alkaline protease by a Bacillus species isolate. Bioresource Technology, 67, 201-203.
  • Mobarak-Qamsari, E., Kasra-Kermanshahi, R., Moosavi-Nejad, Z., (2011). Isolation and identification of a novel, lipase-producing bacterium, Pseudomnas aeruginosa KM110. Iranian Journal of Microbiology, 3(2), 92-98.
  • Oberoi, R., Beg, Q.K., Puri, S., Saxena, R.K., Gupta, R., (2001). Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp. World Journal of Microbiology&Biotechnology, 17, 493-497.
  • Sangeetha, R., Geetha, A., Arulpandi, I., (2008). Optimization of protease and lipase production by Bacillus pumilus SG 2 isolated from an industrial effluent. The Internet Journal of Microbiology, 5(2), 1-8.
  • Rapp, P., Backhaus, S., (1992). Formation of extracellular lipase by filamentous fungi, yeasts and bacteria. Enzyme and Microbial Technology, 14, 938-943.
  • Rathi, P., Saxena, R.K., Gupta, R., (2001). A novel alkaline lipase from Burkholderia cepacia for detergent formulation. Process Biochemistry, 37, 187-192.
  • Remya, M., Vijayakumar, R., (2008). Isolation and characterization of marine antagonistic actinomycetes from west coast of India. Medicine and Biology, 15(1), 13-19.
  • Sztajer, H., Maliszewska, I., Wieczorek, J., (1988). Production of exogenous lipase by bacteria, fungi and actinomycetes. Enzyme and Microbial Technology, 10, 492-497.
  • Ülker, S., Özel, A., Çolak, A., Alpay Karaoğlu, Ş., (2011). Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil. Turkish Journal of Biology, 35, 543-550.
  • Wiseman, A., (1987). Handbook of Enzymes Biotechnology, Second Edition. Ellis Horwood Limited: Chichester.
There are 19 citations in total.

Details

Subjects Engineering
Journal Section Articles
Authors

Kadriye Özcan

Cengiz Çorbacı

Publication Date December 15, 2017
Published in Issue Year 2017 Volume: 7 Issue: 2

Cite

APA Özcan, K., & Çorbacı, C. (2017). Streptomyces sp. K22 ve K30 Suşlarından Lipaz ve Proteaz Enzim Üretimi. Karadeniz Fen Bilimleri Dergisi, 7(2), 128-135. https://doi.org/10.31466/kfbd.343293