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Year 2020, Volume 10 , Issue 2, Pages 290 - 301 2020-12-15

Vişne Sapı Ekstraktlarının in vitro Metotlarla Antioksidan Aktivitelerinin Belirlenmesi
Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods

Filiz UÇAN TÜRKMEN [1] , Hatice Aysun MERCİMEK TAKCI [2] , Fatma Esen SARIGÜLLÜ ÖNALAN [3]

Abstract

Bu çalışmada, vişne (Prunus cerasus L.) sapı ekstrelerinin antioksidan aktiviteleri β-karoten/linoleik asit, metal şelatlama, güç azaltma, DPPH radikal süpürme, ABTS yöntemi ve TEAC metodu kullanılarak araştırılmıştır. Bu amaçla, saplar kurutulduktan sonra damıtılmış su, metanol, etil asetat ve hekzan çözücüleri kullanılarak ayrı ayrı özütlenmiş ve ince toz haline getirilmiştir. Farklı konsantrasyonlarda (100-1000 μg/mL) hazırlanan ekstrelerin antioksidan aktiviteleri a-tokoferol, askorbik asit, BHT ve BHA standartları ile karşılaştırılmıştır. Vişne sapı metanol ekstresininen yüksek verime (% 22) sahip olduğu belirlenmiştir. 100-1000 µg/mL konsantrasyonlarda metanol ekstresinin şelatlama aktiviteleri (Fe2+)%18.45 ile 67.75 arasında değişmektedir. Bununla birlikte, tüm test ekstrelerinin kenetleme aktiviteleri standartlardan daha düşük olmuştur. Hekzan hariç tüm ekstrelerin indirgeme gücü konsantrasyondaki artışa bağlı olarak artmıştır. Metanol ekstresinin DPPH radikal temizleme etkinliği,%14.57 ila85.78 arasında değişmiştir. En düşük EC50 değeri, metanol ekstresiiçin 0.494 µg/mL olarak belirlenmiştir. Ancak, linoleik asit oksidasyonunun en yüksek yüzde inhibisyonu, hekzan ekstresinde(% 57.22) bulunmuştur. Metanol ekstresinin Trolox eşdeğeri antioksidan kapasite değerleri 15.43 ila 20.54 µM Troloxeşdeğeri /10 g arasında değişmektedir. Metanol ekstresinin antioksidan aktiviteler üzerinde doza bağlı bir inhibisyon gösterdiği belirlenmiştir.
In this study, the antioxidant activities of sour cherry (Prunus cerasus L.) stalk extracts were investigated using β-caroten/linoleic acid, metal chelating, reducing power, DPPH radical scavenging, ABTS method and TEAC method. For this purpose the stalks were extracted separately by using distilled water, methanol, ethyl acetate and hexane solvents following dried and ground to fine powder. The antioxidant activities of extracts prepared at the different concentration (100-1000 μg/mL) were compared with α-tocopherol, ascorbic acid, BHT and BHA standards. The methanol extract of stalk had the highest yield (22%). Chelating activities (Fe2+) of methanol extract at 100-1000 μg/mL concentrations ranged between 18.45 and 67.75%. However, chelating activities of all test extracts were found lower than standards. The reducing power of all extracts except hexane increased depending upon the increase in concentration. DPPH radical scavenging activity of methanol extract varied in the range of 14.57 to 85.78%. The lowest EC50 value was determined as 0.494 µg/mL for methanol extract. But, the highest inhibition percentage of linoleic acid oxidation was found in hexane extract (57.22%). TEAC values of methanol extract ranged from 15.43 to 20.54 µM Trolox equivalent/10 g. It was determined that methanol extract showed a dose-dependent inhibition on the antioxidant activities.

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Details

Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-3653-9433
Author: Filiz UÇAN TÜRKMEN (Primary Author)
Institution: KILIS 7 ARALIK UNIVERSITY
Country: Turkey

Orcid: 0000-0002-5394-4959
Author: Hatice Aysun MERCİMEK TAKCI
Institution: KILIS 7 ARALIK UNIVERSITY
Country: Turkey

Orcid: 0000-0002-1374-4338
Author: Fatma Esen SARIGÜLLÜ ÖNALAN
Institution: KILIS 7 ARALIK UNIVERSITY
Country: Turkey
Supporting Institution Kilis 7 Aralık Üniversitesi BAP birimi
Project Number 11932
Thanks This study was supported by Kilis 7 Aralık University Research Fund (Project number: 11932).
Dates

Publication Date : December 15, 2020

Cite

Bibtex @research article { kfbd722885, journal = {Karadeniz Fen Bilimleri Dergisi}, issn = {1309-4726}, eissn = {2564-7377}, address = {Giresun Üniversitesi Fen Bilimleri Enstitüsü Güre Yerleşkesi 28200 / Giresun}, publisher = {Giresun University}, year = {2020}, volume = {10}, pages = {290 - 301}, doi = {10.31466/kfbd.722885}, title = {Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods}, key = {cite}, author = {Uçan Türkmen, Filiz and Merci̇mek Takcı, Hatice Aysun and Sarıgüllü Önalan, Fatma Esen} }
APA Uçan Türkmen, F , Merci̇mek Takcı, H , Sarıgüllü Önalan, F . (2020). Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods . Karadeniz Fen Bilimleri Dergisi , 10 (2) , 290-301 . DOI: 10.31466/kfbd.722885
MLA Uçan Türkmen, F , Merci̇mek Takcı, H , Sarıgüllü Önalan, F . "Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods" . Karadeniz Fen Bilimleri Dergisi 10 (2020 ): 290-301 <https://dergipark.org.tr/en/pub/kfbd/issue/57177/722885>
Chicago Uçan Türkmen, F , Merci̇mek Takcı, H , Sarıgüllü Önalan, F . "Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods". Karadeniz Fen Bilimleri Dergisi 10 (2020 ): 290-301
RIS TY - JOUR T1 - Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods AU - Filiz Uçan Türkmen , Hatice Aysun Merci̇mek Takcı , Fatma Esen Sarıgüllü Önalan Y1 - 2020 PY - 2020 N1 - doi: 10.31466/kfbd.722885 DO - 10.31466/kfbd.722885 T2 - Karadeniz Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 290 EP - 301 VL - 10 IS - 2 SN - 1309-4726-2564-7377 M3 - doi: 10.31466/kfbd.722885 UR - https://doi.org/10.31466/kfbd.722885 Y2 - 2020 ER -
EndNote %0 Karadeniz Fen Bilimleri Dergisi Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods %A Filiz Uçan Türkmen , Hatice Aysun Merci̇mek Takcı , Fatma Esen Sarıgüllü Önalan %T Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods %D 2020 %J Karadeniz Fen Bilimleri Dergisi %P 1309-4726-2564-7377 %V 10 %N 2 %R doi: 10.31466/kfbd.722885 %U 10.31466/kfbd.722885
ISNAD Uçan Türkmen, Filiz , Merci̇mek Takcı, Hatice Aysun , Sarıgüllü Önalan, Fatma Esen . "Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods". Karadeniz Fen Bilimleri Dergisi 10 / 2 (December 2020): 290-301 . https://doi.org/10.31466/kfbd.722885
AMA Uçan Türkmen F , Merci̇mek Takcı H , Sarıgüllü Önalan F . Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods. KFBD. 2020; 10(2): 290-301.
Vancouver Uçan Türkmen F , Merci̇mek Takcı H , Sarıgüllü Önalan F . Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods. Karadeniz Fen Bilimleri Dergisi. 2020; 10(2): 290-301.
IEEE F. Uçan Türkmen , H. Merci̇mek Takcı and F. Sarıgüllü Önalan , "Evaluation of Antioxidant Activity of Sour Cherry Stalk Extracts by in Vitro Methods", Karadeniz Fen Bilimleri Dergisi, vol. 10, no. 2, pp. 290-301, Dec. 2020, doi:10.31466/kfbd.722885
Authors of the Article
Filiz UÇAN TÜRKMEN [1]
Hatice Aysun MERCİMEK TAKCI [2]
Fatma Esen SARIGÜLLÜ ÖNALAN [3]
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