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Doğal Zeolitlerin Okratoksin A Adsorbsiyon Kapasitelerinin Geliştirilmesi ve Üzüm Suyundan Okratoksin A Uzaklaştırma İşleminde Kullanılması

Year 2020, Volume: 10 Issue: 2, 360 - 379, 15.12.2020
https://doi.org/10.31466/kfbd.807995

Abstract

Okratoksin A (OTA), üzüm ve üzüm ürünlerinde en sık rastlanan mikotoksindir. Zeolitlerin gıda ve yemlerde bulunan mikotoksinlerin detoksifikasyonlarında kullanımı son yıllarda önemli bir araştırma konusu haline gelmiştir. Çalışmada, iki farklı boyutta (100 μm ve 1-3 mm) doğal zeolit (DZ), OTA adsorbsiyon kapasitesinin artırılması amacıyla Oktadesil trimetil amonyum bromid (ODTMA) ile muamele edilerek modifiye organo-zeolit (MZ) sentezi gerçekleştirilmiştir. Sentezlenen MZ’lerin OTA adsorbsiyon kapasiteleri DZ’ler ile birlikte değerlendirilmiştir. Bu amaçla pH’ı 3’e ayarlanmış OTA içeren tampon çözeltilere ilave edilen DZ ve MZ’lerin maksimum adsorbsiyon gerçekleştirdiği miktar, süre ve sıcaklıklar belirlenerek, üzüm suyundan OTA adsorbsiyonu için optimum koşullar belirlenmiştir. Gerek tampon gerekse de üzüm suyu denemelerinde MZ’ler DZ’lere göre daha iyi adsorbsiyon sonuçları vermişlerdir. 10 ng.mL-1 OTA içeren üzüm suyu, 5% oranında ilave edilen DZ ve MZ’ler eşliğinde 45 °C sıcaklıkta 120 dakika karıştırma süresine tabi tutulduğunda en yüksek adsorbsiyon oranı 89.68%±1.48 ile MT örneğinde gerçekleşmiştir. 1-3 mm boyutundaki zeolit tipleri tüm denemelerde toz formunda olanlara göre daha düşük adsorbsiyon yeteneği göstermiştir. DZ’lerin yüzey yapısının modifiye edilerek OTA adsorbsiyon kapasitesinin artırılması ileüzüm suyundan OTA uzaklaştırılmasında aktif karbonlara alternatif olarak kullanılabileceği düşünülmektedir.

Supporting Institution

Giresun Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FEN-BAP-A-140316-35

References

  • Akova, Y., (2009). Kuru Üzüm, T. C. Başbakanlık Dış Ticaret Müsteşarlığı İhracatı Geliştirme Etüd Merkezi Sektör Raporu, 2009.
  • Battilani, P., Giorni, P., Pietri, A. (2003). Epidemiology of Toxin- Producing Fungi and Ochratoxin A Occurrence in Grape. European Journal of Plant Pathology, 109, 715–722.
  • Battilani, P., Magan, N., Logrieco, A. (2006). European research on ochratoxin A in grapes and wine. Int J Food Microbiol. 111:2–4.
  • Bilgin, Ö., Koç. B. (2013). Doğal zeolitlerin çevre kirliliği kontrolünde kullanımı. Turkish Journal of Scientific Reviews, 6(1), 169-174.
  • Breyden, W. L. (2007). Mycotoxins in The Food Chain: Human Health Implications, Asia Pac. J. Clin. Nutr. (16), 95- 101.
  • Cabaroğlu, T., Yılmaztekin, M. (2006, Kasım). Üzümün bileşimi ve insan sağlığı üzerine etkileri. Buldan Sempozyumu (s. 999-1004). Denizli. Pamukkale Üniversitesi.
  • Daković, A., Tomašević-Čanović, M., Rottinghaus, G., Dondur, V., Mašić, Z. (2003). Adsorption of ochratoxin A on octadecyldimethyl benzyl ammonium exchanged-clinoptilolite-heulandite tuff. Colloids and Surfaces B: Biointerfaces, 30(1-2), 157-165.
  • European Comission. (2006). Setting maximum levels for certain contaminants in foodstuffs (Text with EEA relevance). Official Journal of the European Union, No 1881.
  • Jackson, L. S., Al-Taher, F. (2008). Factors Affecting Mycotoxin Productions in Fruits, Mycotoxins in Fruits and Vegetables ( Barkai- Golan, R., Paster, N. Eds.), Academic Pres, UK, pp75- 105.
  • Jha, B., Singh, D. N. (2016). Fly ash zeolites, Advanced Structured Materials, Chaper 2, Basics of zeolites.
  • Kabak, B., Var, I. (2014). Mikotoksinlerin Detoksifikasyonunda Adsorban Maddelerin Kullanımı. Gıda ve Yem Bilimi Teknolojisi Dergisi, 8,49-56.
  • Lamprecht, M., Bogner, S., Steinbauer, K., Schuetz, B., Greilberger, J. F., Leber, B., Oberwinkler, T. (2015). Effects of zeolite supplementation on parameters of intestinal barrier integrity, inflammation, redoxbiology and performance in aerobically trained subjects. Journal of the International Society of Sports Nutrition, 12(1), 40.
  • Malir, F., Ostry, V., Pfohl-Leszkowicz, A., Malir, J., & Toman, J. (2016). Ochratoxin A: 50 years of research. Toxins, 8(7), 191.
  • Margeta, K., Logar, N. Z., Šiljeg, M., Farkaš, A. (2013). Natural zeolites in water treatment–how effective is their use. Water treatment, 5, 81-112.
  • Mercurio, M., Mercurio, V., de'Gennaro, B., de'Gennaro, M., Grifa, C., Langella, A.,Morra, V. (2010). Natural zeolites and white wines from Campania region (Southern Italy): a new contribution for solving some oenological problems. Periodico di Mineralogia, 79(1), 95-112.
  • Narayanasamy, P. (2006). Postharvest Pathogens and Disease Management, John Wiley & Sons, Inc., Hoboken, New Jersey, USA, 582p, 2006.
  • Pasinli T., Henden E. (2013). Solid Phase Extraction of Zearalenone (ZEN) from Beer Samples Using Natural Zeolite Clinoptilolite and Organo-Zeolites Prior to HPLC Determination. Ekoloji 22(89): 57-66.
  • RASSF, 2020: https://webgate.ec.europa.eu/rasff-window/portal/?event=searchResultList (Erişim Tarihi, Ekim 2020)
  • Seo, J. A., Yu, J. H. (2005). Toxigenic Fungi and Mycotoxins. In Handbook of Industrial Mycology, Edited by Z. An., Marcell Dekker Inc, 270 Madison Avenue, New York, NY 10016, 749p.
  • Sherif, S. O., Salama, E.E., & Abdel-Wahhab, M. A. (2009). Mycotoxins and child health: The need for health risk assessment. International journal of hygiene and environmental health, 212(4), 347-368.
  • Tomašević-Čanović, M., Daković, A., Rottinghaus, G., Matijašević, S., & Đuričić, M. (2003). Surfactant modified zeolites––new efficient adsorbents for mycotoxins. Microporous and mesoporous materials, 61(1), 173-180.
  • Top, A., Ülkü, S. (2004). Silver, zinc, and copper exchange in a Na-clinoptilolite and resulting effect on antibacterial activity. Applied Clay Science, 27, 13-19.
  • Var, I., Kabak, B., Erginkaya, Z. (2008). Reduction in ochratoxin A levels in white wine, following treatment with activated carbon and sodium bentonite. Food Control, 19(6), 592-598.
  • Varga, J., Kocsubé, S., Péteri, Z., Vágvölgyi, C., Tóth, B. (2010). Chemical, physical and biological approaches to prevent ochratoxin induced toxicoses in humans and animals. Toxins, 2(7), 1718-1750.
  • Wasielewski, S., Rott, E., Minke, R., Steinmetz, H. (2018). Evaluation of different clinoptilolite zeolites as adsorbent for ammonium removal from highly concentrated synthetic wastewater. Water, 10(5), 584.
  • Zimmerli, B., Dick, R. (1996). Ochratoxin A in table wine and grape-juice: occurrence and risk assessment. Food Addit. Contam. 13:655–668.

Improvement of Ochratoxin A Adsorption Capacity of Natural Zeolites and Its Use in the Removal of Ochratoxin A from Grape Juice

Year 2020, Volume: 10 Issue: 2, 360 - 379, 15.12.2020
https://doi.org/10.31466/kfbd.807995

Abstract

Octratoxin A (OTA) is the most common mycotoxin in grape and grape products. The use of zeolites in detoxification of mycotoxins in food and feed has become an important research topic in recent years. In this study, Modified organo-zeolite (MZ) synthesis was carried out by treating natural zeolite (DZ) in two different sizes (100 μm and 1-3 mm) with octadecyl trimethyl ammonium bromide (ODTMA) in order to raise the OTA adsorption capacity. OTA adsorption capacities of synthesized MZs were evaluated together with DZ. For this purpose, optimum conditions for OTA adsorption from grape juice were determined by determining the amount, time and temperature at which DZ and MZ added to buffer solutions containing OTA adjusted to pH 3 were subjected to maximum adsorption. In both buffer and grape juice trials, MZs gave better adsorption results than DZs. Grape juice containing 10 ng.mL-1 OTA was subjected to stirring for 120 min at 45 ° C in the presence of DZ and MZ added at 5%, resulting in the highest adsorption rate of 89.68% ± 1.48 for MT added samples. Zeolite types 1-3 mm in size showed lower adsorption ability than those in powder form in all experiments. It is thought that the surface structure of DZs can be modified to increase the OTA adsorption capacity and be used as an alternative to activated carbons in the removal of OTA from grape juice.

Project Number

FEN-BAP-A-140316-35

References

  • Akova, Y., (2009). Kuru Üzüm, T. C. Başbakanlık Dış Ticaret Müsteşarlığı İhracatı Geliştirme Etüd Merkezi Sektör Raporu, 2009.
  • Battilani, P., Giorni, P., Pietri, A. (2003). Epidemiology of Toxin- Producing Fungi and Ochratoxin A Occurrence in Grape. European Journal of Plant Pathology, 109, 715–722.
  • Battilani, P., Magan, N., Logrieco, A. (2006). European research on ochratoxin A in grapes and wine. Int J Food Microbiol. 111:2–4.
  • Bilgin, Ö., Koç. B. (2013). Doğal zeolitlerin çevre kirliliği kontrolünde kullanımı. Turkish Journal of Scientific Reviews, 6(1), 169-174.
  • Breyden, W. L. (2007). Mycotoxins in The Food Chain: Human Health Implications, Asia Pac. J. Clin. Nutr. (16), 95- 101.
  • Cabaroğlu, T., Yılmaztekin, M. (2006, Kasım). Üzümün bileşimi ve insan sağlığı üzerine etkileri. Buldan Sempozyumu (s. 999-1004). Denizli. Pamukkale Üniversitesi.
  • Daković, A., Tomašević-Čanović, M., Rottinghaus, G., Dondur, V., Mašić, Z. (2003). Adsorption of ochratoxin A on octadecyldimethyl benzyl ammonium exchanged-clinoptilolite-heulandite tuff. Colloids and Surfaces B: Biointerfaces, 30(1-2), 157-165.
  • European Comission. (2006). Setting maximum levels for certain contaminants in foodstuffs (Text with EEA relevance). Official Journal of the European Union, No 1881.
  • Jackson, L. S., Al-Taher, F. (2008). Factors Affecting Mycotoxin Productions in Fruits, Mycotoxins in Fruits and Vegetables ( Barkai- Golan, R., Paster, N. Eds.), Academic Pres, UK, pp75- 105.
  • Jha, B., Singh, D. N. (2016). Fly ash zeolites, Advanced Structured Materials, Chaper 2, Basics of zeolites.
  • Kabak, B., Var, I. (2014). Mikotoksinlerin Detoksifikasyonunda Adsorban Maddelerin Kullanımı. Gıda ve Yem Bilimi Teknolojisi Dergisi, 8,49-56.
  • Lamprecht, M., Bogner, S., Steinbauer, K., Schuetz, B., Greilberger, J. F., Leber, B., Oberwinkler, T. (2015). Effects of zeolite supplementation on parameters of intestinal barrier integrity, inflammation, redoxbiology and performance in aerobically trained subjects. Journal of the International Society of Sports Nutrition, 12(1), 40.
  • Malir, F., Ostry, V., Pfohl-Leszkowicz, A., Malir, J., & Toman, J. (2016). Ochratoxin A: 50 years of research. Toxins, 8(7), 191.
  • Margeta, K., Logar, N. Z., Šiljeg, M., Farkaš, A. (2013). Natural zeolites in water treatment–how effective is their use. Water treatment, 5, 81-112.
  • Mercurio, M., Mercurio, V., de'Gennaro, B., de'Gennaro, M., Grifa, C., Langella, A.,Morra, V. (2010). Natural zeolites and white wines from Campania region (Southern Italy): a new contribution for solving some oenological problems. Periodico di Mineralogia, 79(1), 95-112.
  • Narayanasamy, P. (2006). Postharvest Pathogens and Disease Management, John Wiley & Sons, Inc., Hoboken, New Jersey, USA, 582p, 2006.
  • Pasinli T., Henden E. (2013). Solid Phase Extraction of Zearalenone (ZEN) from Beer Samples Using Natural Zeolite Clinoptilolite and Organo-Zeolites Prior to HPLC Determination. Ekoloji 22(89): 57-66.
  • RASSF, 2020: https://webgate.ec.europa.eu/rasff-window/portal/?event=searchResultList (Erişim Tarihi, Ekim 2020)
  • Seo, J. A., Yu, J. H. (2005). Toxigenic Fungi and Mycotoxins. In Handbook of Industrial Mycology, Edited by Z. An., Marcell Dekker Inc, 270 Madison Avenue, New York, NY 10016, 749p.
  • Sherif, S. O., Salama, E.E., & Abdel-Wahhab, M. A. (2009). Mycotoxins and child health: The need for health risk assessment. International journal of hygiene and environmental health, 212(4), 347-368.
  • Tomašević-Čanović, M., Daković, A., Rottinghaus, G., Matijašević, S., & Đuričić, M. (2003). Surfactant modified zeolites––new efficient adsorbents for mycotoxins. Microporous and mesoporous materials, 61(1), 173-180.
  • Top, A., Ülkü, S. (2004). Silver, zinc, and copper exchange in a Na-clinoptilolite and resulting effect on antibacterial activity. Applied Clay Science, 27, 13-19.
  • Var, I., Kabak, B., Erginkaya, Z. (2008). Reduction in ochratoxin A levels in white wine, following treatment with activated carbon and sodium bentonite. Food Control, 19(6), 592-598.
  • Varga, J., Kocsubé, S., Péteri, Z., Vágvölgyi, C., Tóth, B. (2010). Chemical, physical and biological approaches to prevent ochratoxin induced toxicoses in humans and animals. Toxins, 2(7), 1718-1750.
  • Wasielewski, S., Rott, E., Minke, R., Steinmetz, H. (2018). Evaluation of different clinoptilolite zeolites as adsorbent for ammonium removal from highly concentrated synthetic wastewater. Water, 10(5), 584.
  • Zimmerli, B., Dick, R. (1996). Ochratoxin A in table wine and grape-juice: occurrence and risk assessment. Food Addit. Contam. 13:655–668.
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Articles
Authors

Levent Şen 0000-0002-4979-7466

Project Number FEN-BAP-A-140316-35
Publication Date December 15, 2020
Published in Issue Year 2020 Volume: 10 Issue: 2

Cite

APA Şen, L. (2020). Doğal Zeolitlerin Okratoksin A Adsorbsiyon Kapasitelerinin Geliştirilmesi ve Üzüm Suyundan Okratoksin A Uzaklaştırma İşleminde Kullanılması. Karadeniz Fen Bilimleri Dergisi, 10(2), 360-379. https://doi.org/10.31466/kfbd.807995