Research Article
PDF Mendeley EndNote BibTex Cite

Effects of Grape Seed and Soybean Extracts on Lipid Peroxidation in Streptozotocin Induced Rats

Year 2022, Volume 3, Issue 1, 1 - 9, 27.04.2022

Abstract

Objective: Recently, the importance of lipid peroxidation in diabetes mellitus, which is characterized by insufficient insulin secretion and impacts, has been reported. To treat this disease, studies have applied herbal treatments and antioxidant agents. The aim of this study determine the possible effects of grape seed and soybean extracts on lipid peroxidation in streptozotocin (STZ)-induced diabetic rats. Method: The rats were divided into 8 groups: control, diabetic control, and 6 extract groups [grape seed (100 mg/kg, 200 mg/kg, and 400 mg/kg dosages) and soybean (100 mg/kg, 200 mg/kg, and 400 mg/kg dosages)]. The first group (control group) was fed a normal diet. The diabetic groups (groups 2, 3, 4, 5, 6, 7, and 8) were fed a high-fat diet, and Diabetes Mellitus was induced by a single intraperitoneal injection of 35 mg/kg STZ. Afterward, extracts were given orally at the indicated dosages to the corresponding treatment groups for 28 days using a gastric tube. At the end of the treatment, liver and pancreatic tissues were collected, and lipid peroxidation parameters and malondialdehyde (MDA) and glutathione (GSH) levels were measured by a spectrophotometric method. Results: For both tissues, MDA levels in the diabetic control group were high, while GSH levels were low. In the treatment groups, MDA levels decreased and GSH levels increased dose-dependently (p < 0.05). Conclusions: According to our results, grape seed and soy extracts can prevent damage caused by lipid peroxidation in diabetes.

References

  • 1. Eckel, R. H., Kahn, S. E., Ferrannini, E., Goldfine, A. B., Nathan, D. M., Schwartz, M. W., Smith, R. J., & Smith, S. R. (2011). Obesity and type 2 diabetes: what can be unified and what needs to be individualized?. J Clin Endocrinol Metab 96:1654-63. https://doi.org/10.1210/jc.2011-0585
  • 2. Armagan, A., Uz, E., Yilmaz, H. R., Soyupek, S., Oksay, T., & Ozcelik, N. (2006). Effects of melatonin on lipid peroxidation and antioxidant enzymes in streptozotocin‐induced diabetic rat testis. Asian J Androl 8:595-600. https://doi.org/10.1111/j.1745-7262.2006.00177.x
  • 3. Tsikas, D. (2017). Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: analytical and biological challenges. Anal Biochem 524:13-30. https://doi.org/10.1016/j.ab.2016.10.021
  • 4. Yaprak, M. (1998). Akut miyokart enfarktüsünde biyokimyasal parametreler ve antioksidan sistemle ilişkisi. Doktora Tezi, Çukurova Üniversitesi
  • 5. Al-Rawi, N. H. (2011). Oxidative stress, antioxidant status and lipid profile in the saliva of type 2 diabetics. Diabetes Vasc Dis Res 8:22-28. https://doi.org/10.1177/1479164110390243
  • 6. Donma, O. (2012). Reaktif Oksijen Türevleri ve Antioksidan Sistem. İstanbul Üniversitesi Basım Ve Yayınevi, İstanbul
  • 7. Harman, D. (1995). Role of antioxidant nutrients in aging. Overview 18:51-62. https://doi.org/10.1007/BF02432519
  • 8. Sies, H. (1996). Antioxidants in Diseases Mecanism and Therapy. Academic Press, USA
  • 9. Cadenzas, E., & Packer, L. (1996). Hand Book of Antioxidants. Plenum, USA
  • 10. Forman, H. J., Zhang, H., & Rinna, A. (2009). Glutathione: overview of its protective roles, measurement, and biosynthesis. Mol Asp Med 30:1-12. https://doi.org/10.1016/j.mam.2008.08.006
  • 11. Chis, I. C., Ungureanu, M. I., Marton, A., Simedrea, R., Muresan, A., Postescu, I. D., & Decea, N. (2009). Antioxidant effects of a grape seed extract in a rat model of diabetes mellitus. Diabetes Vasc Dis Res 6:200-04. https://doi.org/10.1177/1479164109336692
  • 12. Nassiri‐Asl, M., & Hosseinzadeh, H. (2009). Review of the pharmacological effects of Vitis vinifera (Grape) and its bioactive compounds. Phytother Res 23:1197-204. https://doi.org/10.1002/ptr.2761
  • 13. Mansouri, E., Panahi, M., Ghaffari, M. A., & Ghorbani, A. (2011). Effects of grape seed proanthocyanidin extract on oxidative stress induced by diabetes in rat kidney. Iran Biomed J 15:100.
  • 14. Girard, B., & Mazza, G. (1998). Functional grape and citrus products. Lancaster, USA
  • 15. Liu, K. (2004). Soybeans as a powerhouse of nutrients and phytochemicals. Soybeans as functional foods and ingredients. Champaign, USA
  • 16. Bas, A. L., Demirci, S., Yazihan, N., Uney, K., & Ermis, K. E. (2012). Nerium oleander distillate improves fat and glucose metabolism in high-fat diet-fed streptozotocin-induced diabetic rats. Int J Endocrinol 2012:947187. https://doi.org/10.1155/2012/947187
  • 17. Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351-58. https://doi.org/10.1016/0003-2697(79)90738-3
  • 18. Ellman, G. L. (1959). Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-77. https://doi.org/10.1016/0003-9861(59)90090-6
  • 19. Tiftik, A. M. (1997). Biüret metoduyla total protein tayini. Mimoza, Türkiye
  • 20. Savaş, H. B., Türkkan, A., Yavuz, B., Yiğit, A., Uz, E., Bayram, N. A., & Kale, B. (2016). Vaccinium Myrtillus’ un Deneysel Diyabetik Sıçan Modelinde Antioksidan Sistem ve Lipit Peroksidasyonu Üzerine Etkileri. International Journal of Basic and Clinical Medicine 4(2): 53-9
  • 21. Gray, S. P., & Jandeleit-Dahm, K. (2014). The pathobiology of diabetic vascular complications cardiovascular and kidney disease. J Mol Med 92:441-52. https://doi.org/10.1007/s00109-014-1146-1.
  • 22. Hamamcıoglu, A. (2017). The role of oxidative stress and antioxidants in diabetes mellitus. Turkish Journal of Diabetes and Obesity 1:7-13.
  • 23. Zhang, F., Ye, C., Li, G., Ding, W., Zhou, W., Zhu, H., Chen, G., Luo, T., Guang, M., Liu, Y., Zhang, D., Zheng, S., Yang, J., Gu, Y., Xie, X., & Luo, M. (2003). The rat model of type 2 diabetic mellitus and its glycometabolism characters. Exp Anim 52:401-07. https://doi.org/10.1538/expanim.52.401
  • 24. Ugarte, M., Brown, M., Hollywood, K. A., Cooper, G. J., Bishop, P. N., & Dunn, W. B. (2012). Metabolomic analysis of rat serum in streptozotocin-induced diabetes and after treatment with oral triethylenetetramine (TETA). Genome Med 4:1-15. https://doi.org/10.1186/gm334
  • 25. Yegin, S., & Mert, N. (2013). Investigation on the Hba1c, MDA, GSH-Px and SOD levels in experimentally diabetic rats. Yüzüncü yıl Üniversitesi Veteriner Fakültesi Dergisi 24:51-54.
  • 26. Ergenç, M., Özenoğlu, S., Turan, İ., Özaçmak, V. H., & Özaçmak, H. S. (2017). Diyabetik Sıçanlarda Melatonin Uygulamasının Karaciğer, Böbrek, Mide, Pankreas ve Göz Dokularında Oksidatif Stres Üzerine Etkisi. Türkiye Diyabet ve Obezite Dergisi 1:117-23.
  • 27. Özok, N., & Güneş, İ. (2019). Streptozotosin kaynaklı diyabetik sıçanlarda Arum rupicola'nın in vivo antioksidan potansiyeli. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 8:866-874. https://doi.org/10.17798/bitlisfen.547871
  • 28. Doğanay, S., Trabzon, Ş., Bahtiyar, N., Güzel, D., Eren Ö. A., & Şahin, A. (2020). Streptozotosin ile İndüklenmiş Diyabetik Sıçanlarda Melatoninin Antioksidan Aktivitesi; Kan ve Karaciğer Dokusunda. Sakarya Tıp Dergisi 10(4): 608-614. https://doi.org/10.31832/smj.787622
  • 29. Jayachandran, M., Vinayagam, R., Ambati, R. R., Xu, B., & Chung, S. S. M. (2018). Guava leaf extract diminishes hyperglycemia and oxidative stress, prevents β-cell death, inhibits inflammation, and regulates NF-kB signaling pathway in STZ induced diabetic rats. Biomed Res Int https://doi.org/10.1155/2018/4601649
  • 30. Gupta, R. K., Kesari, A. N., Murthy, P., Chandra, R., Tandon, V., & Watal, G. (2005). Hypoglycemic and antidiabetic effect of ethanolic extract of leaves of Annona squamosa L. in experimental animals. J Ethnopharmacol 99:75-81. https://doi.org/10.1016/j.jep.2005.01.048
  • 31. Naczk, M., & Shahidi, F. (2004). Extraction and analysis of phenolics in food. Journal of chromatography A 1054(1-2):95-111. https://doi.org/10.1016/j.chroma.2004.08.059
  • 32. Güzel, N. (2010). Nar suyu konsantresi üretim aşamalarında prosiyanidinlerdeki değişimler. Yüksek Lisans Tezi, Ankara Üniversitesi
  • 33. Ganjali, Z., Javadian, F., Estakhr, J., & Heidari, A. (2012). Anti-lipidimic and anti-hyperglycemic properties of methanolic extract of grape seed in diabetic rats. International Journal of Animal and Veterinary Advances 4:173-75. https://doi.org/10.4103/0973-1296.93328
  • 34. Şendoğdu, N., Aslan, M., Orhan, D. D., Ergun, F., & Yeşilada, E. (2006). Antidiabetic and antioxidant effects of Vitis vinifera L. leaves in streptozotocin-diabetic rats. Turkish J Pharm Sci 3:7-18.
  • 35. Nilüfer, D., & Boyacıoğlu, D. (2008). Soya ve soya ürünlerinin fonksiyonel gıda bileşenleri. Gıda 33:241-50.
  • 36. Mourad, H. H., El-Kassaby, M. I., El-Hussieny, E. A., Esmail, R. S., Mannaa, F. A., & Khaled, G. (2017). Role of soy protein concentrate on oxidative stress and DNA fragmentation in streptozotocin-induced diabetic rats. J Innov Pharm Biol 4:16-25.
  • 37. Lee, J. S. (2006). Effects of soy protein and genistein on blood glucose, antioxidant enzyme activities, and lipid profile in streptozotocin-induced diabetic rats. Life Sci 79:1578-84. https://doi.org/10.1016/j.lfs.2006.06.030

Streptozotosin Uygulanan Sıçanlarda Lipit Peroksidasyonu Üzerine Üzüm Çekirdeği ve Soya Ekstraktlarının Etkileri

Year 2022, Volume 3, Issue 1, 1 - 9, 27.04.2022

Abstract

Amaç: Son zamanlarda, yetersiz insülin sekresyonu ve etkileri ile karakterize olan diabetes mellitusta lipid peroksidasyonunun önemi bildirilmektedir. Bu hastalığı tedavi etmek için bitkisel tedaviler ve antioksidan ajanlar uygulanmış çalışmalar yapılmıştır. Bu çalışmanın amacı, streptozotosin (STZ) ile indüklenen diyabetik sıçanlarda üzüm çekirdeği ve soya fasulyesi ekstraktlarının lipid peroksidasyonu üzerine olası etkilerini belirlemektir. Gereç ve Yöntem: Sıçanlar 8 gruba ayrıldı: kontrol, diyabetik kontrol ve 6 ekstrakt grubu [üzüm çekirdeği (100 mg/kg, 200 mg/kg ve 400 mg/kg dozları) ve soya fasulyesi (100 mg/kg, 200 mg/kg ve 400 mg/kg dozları)]. Birinci grup (kontrol grubu) normal bir diyetle beslendi. Diyabetik gruplar (grup 2, 3, 4, 5, 6, 7 ve 8) yüksek yağlı bir diyetle beslendi ve Diabetes Mellitus, tek bir intraperitoneal 35 mg/kg STZ enjeksiyonu ile indüklendi. Daha sonra ekstreler, bir gavaj kullanılarak 28 gün boyunca ilgili tedavi gruplarına belirtilen dozlarda oral yoldan verildi. Tedavi sonunda karaciğer ve pankreas dokuları toplandı ve lipit peroksidasyon parametreleri ile malondialdehit (MDA) ve glutatyon (GSH) seviyeleri spektrofotometrik yöntemle ölçüldü. Bulgular: Her iki doku için de diyabetik kontrol grubunda MDA seviyeleri yüksek, GSH seviyeleri düşüktü. Tedavi gruplarında doza bağlı olarak MDA seviyeleri azaldı ve GSH seviyeleri arttı (p < 0.05). Sonuç: Sonuçlara göre üzüm çekirdeği ve soya ekstraktları diyabette lipid peroksidasyonunun neden olduğu hasarı önleyebilir.

References

  • 1. Eckel, R. H., Kahn, S. E., Ferrannini, E., Goldfine, A. B., Nathan, D. M., Schwartz, M. W., Smith, R. J., & Smith, S. R. (2011). Obesity and type 2 diabetes: what can be unified and what needs to be individualized?. J Clin Endocrinol Metab 96:1654-63. https://doi.org/10.1210/jc.2011-0585
  • 2. Armagan, A., Uz, E., Yilmaz, H. R., Soyupek, S., Oksay, T., & Ozcelik, N. (2006). Effects of melatonin on lipid peroxidation and antioxidant enzymes in streptozotocin‐induced diabetic rat testis. Asian J Androl 8:595-600. https://doi.org/10.1111/j.1745-7262.2006.00177.x
  • 3. Tsikas, D. (2017). Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: analytical and biological challenges. Anal Biochem 524:13-30. https://doi.org/10.1016/j.ab.2016.10.021
  • 4. Yaprak, M. (1998). Akut miyokart enfarktüsünde biyokimyasal parametreler ve antioksidan sistemle ilişkisi. Doktora Tezi, Çukurova Üniversitesi
  • 5. Al-Rawi, N. H. (2011). Oxidative stress, antioxidant status and lipid profile in the saliva of type 2 diabetics. Diabetes Vasc Dis Res 8:22-28. https://doi.org/10.1177/1479164110390243
  • 6. Donma, O. (2012). Reaktif Oksijen Türevleri ve Antioksidan Sistem. İstanbul Üniversitesi Basım Ve Yayınevi, İstanbul
  • 7. Harman, D. (1995). Role of antioxidant nutrients in aging. Overview 18:51-62. https://doi.org/10.1007/BF02432519
  • 8. Sies, H. (1996). Antioxidants in Diseases Mecanism and Therapy. Academic Press, USA
  • 9. Cadenzas, E., & Packer, L. (1996). Hand Book of Antioxidants. Plenum, USA
  • 10. Forman, H. J., Zhang, H., & Rinna, A. (2009). Glutathione: overview of its protective roles, measurement, and biosynthesis. Mol Asp Med 30:1-12. https://doi.org/10.1016/j.mam.2008.08.006
  • 11. Chis, I. C., Ungureanu, M. I., Marton, A., Simedrea, R., Muresan, A., Postescu, I. D., & Decea, N. (2009). Antioxidant effects of a grape seed extract in a rat model of diabetes mellitus. Diabetes Vasc Dis Res 6:200-04. https://doi.org/10.1177/1479164109336692
  • 12. Nassiri‐Asl, M., & Hosseinzadeh, H. (2009). Review of the pharmacological effects of Vitis vinifera (Grape) and its bioactive compounds. Phytother Res 23:1197-204. https://doi.org/10.1002/ptr.2761
  • 13. Mansouri, E., Panahi, M., Ghaffari, M. A., & Ghorbani, A. (2011). Effects of grape seed proanthocyanidin extract on oxidative stress induced by diabetes in rat kidney. Iran Biomed J 15:100.
  • 14. Girard, B., & Mazza, G. (1998). Functional grape and citrus products. Lancaster, USA
  • 15. Liu, K. (2004). Soybeans as a powerhouse of nutrients and phytochemicals. Soybeans as functional foods and ingredients. Champaign, USA
  • 16. Bas, A. L., Demirci, S., Yazihan, N., Uney, K., & Ermis, K. E. (2012). Nerium oleander distillate improves fat and glucose metabolism in high-fat diet-fed streptozotocin-induced diabetic rats. Int J Endocrinol 2012:947187. https://doi.org/10.1155/2012/947187
  • 17. Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351-58. https://doi.org/10.1016/0003-2697(79)90738-3
  • 18. Ellman, G. L. (1959). Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-77. https://doi.org/10.1016/0003-9861(59)90090-6
  • 19. Tiftik, A. M. (1997). Biüret metoduyla total protein tayini. Mimoza, Türkiye
  • 20. Savaş, H. B., Türkkan, A., Yavuz, B., Yiğit, A., Uz, E., Bayram, N. A., & Kale, B. (2016). Vaccinium Myrtillus’ un Deneysel Diyabetik Sıçan Modelinde Antioksidan Sistem ve Lipit Peroksidasyonu Üzerine Etkileri. International Journal of Basic and Clinical Medicine 4(2): 53-9
  • 21. Gray, S. P., & Jandeleit-Dahm, K. (2014). The pathobiology of diabetic vascular complications cardiovascular and kidney disease. J Mol Med 92:441-52. https://doi.org/10.1007/s00109-014-1146-1.
  • 22. Hamamcıoglu, A. (2017). The role of oxidative stress and antioxidants in diabetes mellitus. Turkish Journal of Diabetes and Obesity 1:7-13.
  • 23. Zhang, F., Ye, C., Li, G., Ding, W., Zhou, W., Zhu, H., Chen, G., Luo, T., Guang, M., Liu, Y., Zhang, D., Zheng, S., Yang, J., Gu, Y., Xie, X., & Luo, M. (2003). The rat model of type 2 diabetic mellitus and its glycometabolism characters. Exp Anim 52:401-07. https://doi.org/10.1538/expanim.52.401
  • 24. Ugarte, M., Brown, M., Hollywood, K. A., Cooper, G. J., Bishop, P. N., & Dunn, W. B. (2012). Metabolomic analysis of rat serum in streptozotocin-induced diabetes and after treatment with oral triethylenetetramine (TETA). Genome Med 4:1-15. https://doi.org/10.1186/gm334
  • 25. Yegin, S., & Mert, N. (2013). Investigation on the Hba1c, MDA, GSH-Px and SOD levels in experimentally diabetic rats. Yüzüncü yıl Üniversitesi Veteriner Fakültesi Dergisi 24:51-54.
  • 26. Ergenç, M., Özenoğlu, S., Turan, İ., Özaçmak, V. H., & Özaçmak, H. S. (2017). Diyabetik Sıçanlarda Melatonin Uygulamasının Karaciğer, Böbrek, Mide, Pankreas ve Göz Dokularında Oksidatif Stres Üzerine Etkisi. Türkiye Diyabet ve Obezite Dergisi 1:117-23.
  • 27. Özok, N., & Güneş, İ. (2019). Streptozotosin kaynaklı diyabetik sıçanlarda Arum rupicola'nın in vivo antioksidan potansiyeli. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 8:866-874. https://doi.org/10.17798/bitlisfen.547871
  • 28. Doğanay, S., Trabzon, Ş., Bahtiyar, N., Güzel, D., Eren Ö. A., & Şahin, A. (2020). Streptozotosin ile İndüklenmiş Diyabetik Sıçanlarda Melatoninin Antioksidan Aktivitesi; Kan ve Karaciğer Dokusunda. Sakarya Tıp Dergisi 10(4): 608-614. https://doi.org/10.31832/smj.787622
  • 29. Jayachandran, M., Vinayagam, R., Ambati, R. R., Xu, B., & Chung, S. S. M. (2018). Guava leaf extract diminishes hyperglycemia and oxidative stress, prevents β-cell death, inhibits inflammation, and regulates NF-kB signaling pathway in STZ induced diabetic rats. Biomed Res Int https://doi.org/10.1155/2018/4601649
  • 30. Gupta, R. K., Kesari, A. N., Murthy, P., Chandra, R., Tandon, V., & Watal, G. (2005). Hypoglycemic and antidiabetic effect of ethanolic extract of leaves of Annona squamosa L. in experimental animals. J Ethnopharmacol 99:75-81. https://doi.org/10.1016/j.jep.2005.01.048
  • 31. Naczk, M., & Shahidi, F. (2004). Extraction and analysis of phenolics in food. Journal of chromatography A 1054(1-2):95-111. https://doi.org/10.1016/j.chroma.2004.08.059
  • 32. Güzel, N. (2010). Nar suyu konsantresi üretim aşamalarında prosiyanidinlerdeki değişimler. Yüksek Lisans Tezi, Ankara Üniversitesi
  • 33. Ganjali, Z., Javadian, F., Estakhr, J., & Heidari, A. (2012). Anti-lipidimic and anti-hyperglycemic properties of methanolic extract of grape seed in diabetic rats. International Journal of Animal and Veterinary Advances 4:173-75. https://doi.org/10.4103/0973-1296.93328
  • 34. Şendoğdu, N., Aslan, M., Orhan, D. D., Ergun, F., & Yeşilada, E. (2006). Antidiabetic and antioxidant effects of Vitis vinifera L. leaves in streptozotocin-diabetic rats. Turkish J Pharm Sci 3:7-18.
  • 35. Nilüfer, D., & Boyacıoğlu, D. (2008). Soya ve soya ürünlerinin fonksiyonel gıda bileşenleri. Gıda 33:241-50.
  • 36. Mourad, H. H., El-Kassaby, M. I., El-Hussieny, E. A., Esmail, R. S., Mannaa, F. A., & Khaled, G. (2017). Role of soy protein concentrate on oxidative stress and DNA fragmentation in streptozotocin-induced diabetic rats. J Innov Pharm Biol 4:16-25.
  • 37. Lee, J. S. (2006). Effects of soy protein and genistein on blood glucose, antioxidant enzyme activities, and lipid profile in streptozotocin-induced diabetic rats. Life Sci 79:1578-84. https://doi.org/10.1016/j.lfs.2006.06.030

Details

Primary Language English
Subjects Medicine
Journal Section Research Articles
Authors

Elif GÜLBAHÇE MUTLU (Primary Author)
KTO KARATAY ÜNİVERSİTESİ
0000-0003-2391-2152
Türkiye


Emine ARSLAN
SELÇUK ÜNİVERSİTESİ
0000-0002-0782-506X
Türkiye


Hilal ARİKOGLU
SELÇUK ÜNİVERSİTESİ
0000-0002-6600-6603
Türkiye


Kaniye Zeynep ÇALIŞKAN SAK
KTO KARATAY ÜNİVERSİTESİ
0000-0003-0847-1168
Türkiye


Salih VAROL
KTO KARATAY ÜNİVERSİTESİ
0000-0002-9954-6657
Türkiye

Publication Date April 27, 2022
Application Date November 8, 2021
Acceptance Date February 8, 2022
Published in Issue Year 2022, Volume 3, Issue 1

Cite

APA Gülbahçe Mutlu, E. , Arslan, E. , Arikoglu, H. , Çalışkan Sak, K. Z. & Varol, S. (2022). Effects of Grape Seed and Soybean Extracts on Lipid Peroxidation in Streptozotocin Induced Rats . KTO Karatay Üniversitesi Sağlık Bilimleri Dergisi , 3 (1) , 1-9 . Retrieved from https://dergipark.org.tr/en/pub/ktokusbd/issue/69608/1020749